Detection of anti-BP180NC16a and anti-BP230 autoantibodies in blister fluid of patients with bullous pemphigoid: the first survey in Greece

Abstract

Bullous pemphigoid (BP) is an acquired bullous disease with an increasing prevalence among elderly people worldwide, including in Greece. Blister formation in most patients with BP is caused by autoantibodies against structural components of the basement membrane zone of the skin, predominantly BP180NC16a and BP230 antigens on the hemidesmosome adhesion complex. Routine diagnostic methods such as histological examination and direct and indirect immunofluorescence are combined to determine diagnosis. In this study, an ELISA was used to measure levels of both anti-BP180NC16A and anti-BP230 autoantibodies in the blister fluid of 13 patients with newly diagnosed BP, before starting treatment. The aim of the study was to evaluate this method as a diagnostic tool in BP. Our results indicate that blister-fluid examination by ELISA can be a useful tool to diagnose bullous pemphigoid, especially in elderly patients who refuse biopsy or have poor venous access.

Blister fluid is a useful diagnostic tool for diagnosis of vancomycin‐induced linear IgA bullous dermatosis

“…[2][3][4] In addition, ELISA could detect anti-BP180NC16a and anti-BP230 autoantibodies in the blister fluid of BP. 5 In this experiment, we clearly demonstrated that co-incubation of blister fluid and VCM resulted in dermal side IgA deposition on ssIIF and enhanced IgA reactivity to COL7 in ELISA.…”

Detection of anti-basement membrane zone antibodies in the blister fluid in subepidermal autoimmune bullous diseases

“…performed BP enzyme-linked immunosorbent assay (ELISA) (BP 180 and 230) of blister fluid in thirteen newly diagnosed BP patients, before starting treatment. [ 15 ] Antibodies could be detected by ELISA in nine patients (69.2%) in blister fluid in contrast to serum where it could be found in eleven patients (84.6%). Sensitivity of IIF in this group of patients with BP was 61.5% (positive in 8 of 13 patients).…”

Evaluation of the combination of BP180-NC16a enzyme-linked immunosorbent assay and BP230 enzyme-linked immunosorbent assay in the diagnosis of bullous pemphigoid

“…The BP180-NC16a kit uses the NC16a domain of BP180 produced as fusion proteins in a baculovirus expression vector system. [14,15] In addition, the BP230 kit used two recombinant proteins as a solid phase, one for the N-terminus and one for the C-terminus of BP230 produced in a bacterial expression system. [15,16] Following the manufacturer's instructions, serum samples were diluted at 1:101 and all samples were tested in duplicate.…”

“…[14,15] In addition, the BP230 kit used two recombinant proteins as a solid phase, one for the N-terminus and one for the C-terminus of BP230 produced in a bacterial expression system. [15,16] Following the manufacturer's instructions, serum samples were diluted at 1:101 and all samples were tested in duplicate. The absorbance (optical density, OD) of each well was read at 450 nm by an automated microplate reader (Bio-Rad 680, Bio-Rad Laboratories, USA).…”