Detection of Anammox Activity and Diversity of Anammox Bacteria-Related 16S rRNA Genes in Coastal Marine Sediment in Japan

Amano, Teruki; Yoshinaga, Ikuo; Okada, Kentaro; Yamagishi, Takao; Ueda, Seinosuke; Obuchi, Akira; Sako, Yoshihiko; Suwa, Yuichi

doi:10.1264/jsme2.22.232

Cited by 130 publications

(115 citation statements)

References 35 publications

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“…Although Amano et al recently reported that anammox activity and anammox-related bacteria were detected in coastal marine sediments in Japan, 12) the anammox-related bacterial clones detected in this study (Fig. 2) were different from those reported in their paper.…”

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confidence: 99%

Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments

Nakajima

Sakka

Kimura

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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We identified 16S rRNA gene sequences in sediment samples from Ago Bay in Japan, forming a new branch of the anammox group or closely related to anaerobic ammonium oxidizing (anammox) bacterial sequences. Anammox activity in the sediment samples was detected by 15 N tracer assays. These results, along with the results of fluorescence in situ hybridization (FISH) analysis, suggest the presence of anammox bacteria in the marine sediments.

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confidence: 99%

Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments

Nakajima

Sakka

Kimura

et al. 2008

Bioscience, Biotechnology, and Biochemistry

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“…Since the discovery of anammox in the sea bed of a continental shelf slope (29), an increasing number of reports have demonstrated that the pathway is present in various marine ecosystems and may involve in the nitrogen cycle (7,24). A few studies reported anammox activity in freshwater and estuarine sediments (2,3,6,16,18,30) as well as in anoxic water columns of permanently stratified lakes in tropical (25) and temperate areas (9). However, the significance and diversity of anammox bacterial populations in freshwater ecosystems are not fully understood.…”

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Distribution and Diversity of Anaerobic Ammonium Oxidation (Anammox) Bacteria in the Sediment of a Eutrophic Freshwater Lake, Lake Kitaura, Japan

et al. 2011

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Although the emission of N2 via anaerobic ammonium oxidation (anammox) is a key process in the elimination of nitrogenous compounds from aquatic environments, little information is available regarding its significance and the relevant microorganisms (anammox bacteria) in eutrophic freshwater lakes. In the present study, the anammox bacteria in the sediment of a eutrophic lake in Japan, Lake Kitaura, were examined using a 15 N-tracer technique to measure their potential anammox activity. Potential anammox activity was localized to the northern region of the lake where a stable supply of both NH4 + and NO3− existed in the sediment. These results suggest the contribution of anammox bacteria to the total emission of N2 from sediment in this eutrophic lake to not be negligible. Moreover, selective PCR successfully amplified anammox bacteria-related (Brocadiales-related) 16S rRNA genes from sediment samples in which potential anammox activity was observed. The clone libraries consisted of diverse phylotypes except the genus "Scalindua"-lineages, and the lineages of genus "Brocadia" were dominantly recovered, followed by the genus "Kuenenia"-lineages. Most of them, however, were novel and phylogenetically distinguishable from known Brocadiales species. A unique population of anammox bacteria inhabits and potentially contributes to the emission of N2 from Lake Kitaura.

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“…Twelve primer combinations (Table 1; Inqaba Biotec, South Africa) obtained from literature were selected to test for the presence of different anammox species. The 50 μℓ PCR reaction contained 200 μM of each deoxynucleotide triphosphate, 0.25 μM of each primer, 2 mM MgCl 2 , 2.5 U Taq DNA polymerase (Fermentas Life Sciences, EU) and approximately 100 ng of template DNA (adapted from Amano et al, 2007). The PCR amplification was initiated with a 4 min denaturation step at 94°C and concluded with a final 7 min elongation at 72°C.…”

Section: Amplification Of Relevant Gene Sequencesmentioning

confidence: 99%

Anaerobic ammonium oxidation in the old trickling filters at Daspoort Wastewater Treatment Works

Wilsenach¹,

Burke²,

Radebe³

et al. 2014

WSA

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The century-old trickling filters at the Daspoort Wastewater Treatment Works in Pretoria (Gauteng, South Africa) are known for their remarkable removal of nitrogen from municipal wastewater. Our study was conducted to identify the microbiological processes responsible for this phenomenon and to establish whether anammox bacteria may be involved. An aerobic and anaerobic bench top reactor, both inoculated with biofilm-covered stones from one of the filters, were spiked with ammonia-nitrogen (NH 4 + -N) and nitrite-nitrogen (NO 2 − -N). These reactors were subsequently monitored by conducting stoichiometric analyses of chemical oxygen demand (COD), NH 4 + -N, NO 2 − -N, and nitrate-nitrogen (NO 3 − -N). In the aerobic reactor, the COD concentration decreased over the 56 h batch reaction period and nitrification was revealed by a decrease in NH 4 + -N and NO 2 − -N concentrations. However, the NO 3 − -N concentration showed no notable decrease. In contrast, in the anaerobic reactor the concentrations of COD, NH 4 + -N, NO 2ˉ-N, NO 3ˉ-N, as well as total nitrogen decreased during the batch reaction period. The decrease of both the NH 4 + -N and NO 2 − -N concentrations, the latter to zero under anaerobic conditions, suggested that, in addition to heterotrophic denitrification, anaerobic ammonium oxidation (anammox) may also occur in the trickling filter biofilm. This was highlighted by the observation that ammonium removal in the anaerobic reactor stopped as soon as the nitrite concentration became zero. The ratio of nitrite:ammonium removal was 1.33 on average, which conforms to anammox behaviour. Gene sequence analysis was used to test for the possible presence of anammox bacteria in the trickling filter biofilm. Genomic DNA was extracted from trickling filter humus sludge and the polymerase chain reaction (PCR) was used to amplify taxonomically informative 16S rRNA gene sequences, using primers specific for selected anammox species. Subsequent sequence analysis, including using the online Basic Local Alignment Search Tool (BLAST), and constructing a phylogenic tree using a heuristic search strategy for Maximum Parsimony analysis, confirmed the presence of an anammox bacterium closely related to Candidatus 'Brocadia anammoxidans' and Candidatus 'Brocadia fulgida' on the biofilm-covered stones of the Daspoort trickling filters.

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Detection of Anammox Activity and Diversity of Anammox Bacteria-Related 16S rRNA Genes in Coastal Marine Sediment in Japan

Cited by 130 publications

References 35 publications

Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments

Detection of Anaerobic Ammonium-Oxidizing Bacteria in Ago Bay Sediments

Distribution and Diversity of Anaerobic Ammonium Oxidation (Anammox) Bacteria in the Sediment of a Eutrophic Freshwater Lake, Lake Kitaura, Japan

Anaerobic ammonium oxidation in the old trickling filters at Daspoort Wastewater Treatment Works

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