Detection of Allergen-IgE interaction in Allergic Children Through Combined Impedance and ROS Measurements

Abstract

Abstract-The aim of this paper is to provide an alternative and more efficient screening test for allergy with respect to traditional UNICAP and prick skin tests, which are commercially available nowadays. The proposed test is aimed at monitoring the interaction of the IgE protein with the allergen in an allergic subject. The idea relies on the fact that the interaction between the IgE and the corresponding allergen happens through binding mechanisms between the IgE and the allergen structure, changing the ionic content of the serum. This latter can be monitored by impedance measurements and confirmed by reactive oxygen species (ROS) evaluation in leukocyte subpopulations. Therefore, in this paper we propose a novel system for the detection of allergies based on impedance measurements and confirmed by ROS measurements. This type of measurement approach has never been presented in the literature for this purpose. A measurement protocol has been developed to guarantee reproducibility and repeatability of the assay. This paper describes both the instrument-specifically designed for impedance evaluationand the measurement protocols for both methodologies, defining univocally, in this way, the overall measurement technique.

Impedance-Based Living Cell Analysis for Clinical Diagnosis of Type I Allergy

“…With respect to immunoglobulins, which are involved in immune reactions, there are five main classes of heavy chain constant domains. Each class defines the IgM, IgG, IgA, IgD, and IgE isotypes [ 8 ]. Mast cells and basophils express the high-affinity IgE receptors (FcεRI) on their cell surface, and IgE antibodies in serum bind to the IgE receptors.…”

Dynamics of Bioimpedance Parameters on Three Frequencies During Ultrafiltration

“…Надiйнiсть, доступнiсть i нешкiдливiсть бiоiмпедансометрiї дозволяють використовувати ї ї як у теоретичних i експериментальних дослiдженнях, так i у клiнiчнiй медицинi. [11][12][13][15][16][17][18][19][20][21][22][23]. Розвивається напрямок векторної бiоiмпедансометрiї [1,[20][21][22][23][24], який використовується як для оцiнювання нутрiцiйного стану дiалiзних пацiєнтiв i досягнення ними потрiбного рiвня "сухої ваги" [1,21,22], так i дiагностики функцiонального стану в iнших ситуацiях [11,24]…”