Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia

Pérez-Castro, Rosalía; Castellanos, Jaime E.; Olano, Víctor Alberto; Mátiz, María Inés; Jaramillo, Juan Felipe; Vargas, Sandra; Sarmiento, Diana; Stenström, Thor Axel; Overgaard, Hans J.

doi:10.1590/0074-02760150363

Cited by 67 publications

(75 citation statements)

References 27 publications

(37 reference statements)

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“…Las visitas a las veredas y las inspecciones se hicieron entre el 2012 y el 2013, y en todas se encontró el vector. Además, se reportó la circulación de los cuatro serotipos del virus en la zona (10). Se confirmó, además, que el DENV-1 fue el serotipo más prevalente, aunque en algunas viviendas se registraron grupos positivos para dos o más serotipos.…”

Section: Discussionunclassified

“…Recientemente, Olano, et al (8), y Pérez-Castro, et al (10), confirmaron la presencia del mosquito y la circulación de los cuatro serotipos del virus del dengue en las áreas rurales de los municipios de La Mesa y Anapoima, Cundinamarca, En estos municipios, la circulación del virus es muy alta en las zonas urbanas, lo cual puede deberse a la presencia de infecciones asintomáticas por dengue, a fallas en las campañas de prevención y control del vector en dichas zonas, y a la poca atención que se presta al dengue y al mosquito vector en las áreas rurales. Otra explicación de la persistencia del virus es la posibilidad de otras formas de transmisión y circulación del virus en el vector, por ejemplo, la transmisión transovárica o vertical (11,12).…”

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Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

et al. 2017

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Introducción. La incidencia y la prevalencia del dengue en Cundinamarca son elevadas y, recientemente, se detectó Aedes aegypti en algunas áreas rurales del departamento.Objetivo. Evaluar la transmisión transovárica del virus del dengue en larvas y pupas recolectadas en áreas rurales del municipio de Anapoima.Materiales y métodos. Se recolectaron ejemplares vivos en 53 viviendas y se transportaron al laboratorio de Anapoima, donde se clasificaron, se agruparon y se congelaron. Llevadas a Bogotá, se las homogeneizó, se les extrajo el ARN con Trizol®, se las sometió a una reacción en cadena de la polimerasa de transcripción inversa (Reverse Transcription Polymerase Change Reaction, RT-PCR) y a PCR convencional, y los productos amplificados se analizaron en geles de agarosa al 2 %.Resultados. En 54,7 % de las viviendas evaluadas se encontraron formas inmaduras del vector y el serotipo más frecuente fue el DENV-1. Sin embargo, en algunos pools se detectó la presencia simultánea de los serotipos DENV 1 y 2, DENV 1 y 3, y DENV 1 y 4, así como los serotipos DENV 1, 2 y 3.Conclusión. Los resultados confirmaron la transmisión vertical del virus de manera natural en el área rural del municipio, lo cual reafirma la capacidad vectorial de A. aegypti y explica, en parte, la persistencia del virus en la región y la posibilidad de que en la fase adulta el vector lo transmita sin haber consumido sangre infectada. Esta situación aumenta el riesgo de infección por el virus del dengue en Colombia y, por lo tanto, la necesidad de adelantar programas de prevención y control en todas las zonas con presencia del mosquito.

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Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

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“…There were no storm drains in any of the schools; all rainwater gutters were inspected and were negative; septic tanks only contained Culex larvae; and all elevated tanks were treated. In a separate paper we have reported on mosquitoes collected in households near schools [37]. The indoor resting density of Ae .…”

Section: Discussionmentioning

confidence: 99%

“…We also found a high prevalence of DENV infected Ae . aegypti in the study area; 62% of mosquito pools were positive, the estimated individual mosquito infection rate was 4%, and in 74% of the examined households DENV-positive mosquitoes were present [37]. …”

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confidence: 99%

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A Cluster-Randomized Controlled Trial to Reduce Diarrheal Disease and Dengue Entomological Risk Factors in Rural Primary Schools in Colombia

et al. 2016

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BackgroundAs many neglected tropical diseases are co-endemic and have common risk factors, integrated control can efficiently reduce disease burden and relieve resource-strained public health budgets. Diarrheal diseases and dengue fever are major global health problems sharing common risk factors in water storage containers. Where provision of clean water is inadequate, water storage is crucial. Fecal contamination of stored water is a common source of diarrheal illness, but stored water also provides breeding sites for dengue vector mosquitoes. Integrating improved water management and educational strategies for both diseases in the school environment can potentially improve the health situation for students and the larger community. The objective of this trial was to investigate whether interventions targeting diarrhea and dengue risk factors would significantly reduce absence due to diarrheal disease and dengue entomological risk factors in schools.Methodology/Principal FindingsA factorial cluster randomized controlled trial was carried out in 34 rural primary schools (1,301 pupils) in La Mesa and Anapoima municipalities, Cundinamarca, Colombia. Schools were randomized to one of four study arms: diarrhea interventions (DIA), dengue interventions (DEN), combined diarrhea and dengue interventions (DIADEN), and control (CON). Interventions had no apparent effect on pupil school absence due to diarrheal disease (p = 0.45) or on adult female Aedes aegypti density (p = 0.32) (primary outcomes). However, the dengue interventions reduced the Breteau Index on average by 78% (p = 0.029), with Breteau indices of 10.8 and 6.2 in the DEN and DIADEN arms, respectively compared to 37.5 and 46.9 in the DIA and CON arms, respectively. The diarrhea interventions improved water quality as assessed by the amount of Escherichia coli colony forming units (CFU); the ratio of Williams mean E. coli CFU being 0.22, or 78% reduction (p = 0.008).Conclusions/SignificanceIntegrated control of dengue and diarrhea has never been conducted before. This trial presents an example for application of control strategies that may affect both diseases and the first study to apply such an approach in school settings. The interventions were well received and highly appreciated by students and teachers. An apparent absence of effect in primary outcome indicators could be the result of pupils being exposed to risk factors outside the school area and mosquitoes flying in from nearby uncontrolled breeding sites. Integrated interventions targeting these diseases in a school context remain promising because of the reduced mosquito breeding and improved water quality, as well as educational benefits. However, to improve outcomes in future integrated approaches, simultaneous interventions in communities, in addition to schools, should be considered; using appropriate combinations of site-specific, effective, acceptable, and affordable interventions.Trial RegistrationClinicalTrials.gov no. ISRCTN40195031

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Potential current and future distribution for Aedes aegypti and Aedes albopictus in Colombia: important disease vectors

Mejía-Jurado,

Echeverry-Cárdenas,

Aguirre-Obando

2024

Biol Invasions

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Invasive mosquitoes, Aedes aegypti (African) and Aedes albopictus (Asian), serve as global vectors for diseases such as dengue, Zika, and chikungunya, and they are currently present in Colombia. Consequently, the aim of this study was to assess the potential geographic distribution of these A. aegypti and A. albopictus vectors under both present and future environmental conditions in Colombia. To achieve this, a dataset was compiled using global records for each species, with those from Colombia being used for model validation. These records, in conjunction with layers of bioclimatic variables, were incorporated into ecological niche models. Predictions of potential distribution were made with the Maxent algorithm, using the Wallace EcoMod application for current conditions and the Java MaxEnt software for future projections, which represented three climate change scenarios (SSP245, SSP370, and SSP585) for two time periods (2041–2060 and 2081–2100). Additionally, niche overlap was calculated, and the suitable habitat range and population at risk in Colombia were quantified for current predictions. It is suggested that both species share ideal areas in the country with an overlap of 0.85, covering over 70% of the country's territory and reaching altitudes higher than 2,500 m, potentially affecting at least 45% of the population. In the future for both species it is estimated that, with increasing temperatures, the potential distribution could decrease, but they could invade areas with altitudes above 3,500 m (colder). It is concluded that, currently, A. aegypti and A. albopictus could have similar geographic distribution in Colombia and that, in the following years, climate change could lead to latitudinal and altitudinal variations in the distribution areas of these vectors.

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Detection of all four dengue serotypes in Aedes aegypti female mosquitoes collected in a rural area in Colombia

Cited by 67 publications

References 27 publications

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

Dengue virus detection in Aedes aegypti larvae and pupae collected in rural areas of Anapoima, Cundinamarca, Colombia

A Cluster-Randomized Controlled Trial to Reduce Diarrheal Disease and Dengue Entomological Risk Factors in Rural Primary Schools in Colombia

Potential current and future distribution for Aedes aegypti and Aedes albopictus in Colombia: important disease vectors

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