Detection of Acidithiobacillus ferrooxidans in acid mine drainage environments using fluorescent in situ hybridization (FISH)

Quantum‐Dot‐Labeled DNA Probes for Fluorescence In Situ Hybridization (FISH) in the Microorganism Escherichia coli

“…[14] Fluorescence in situ hybridization (FISH) is an important nucleic acid-based technique for direct visualization of microorganisms, which is normally used for the investigation of microbial diversity and complex microbial communities, and for the detection of microorganisms in biomedical and environmental samples, including pathogens and fungi. [15][16][17][18] Commonly used dyes are usually too photobleachable to give perfect photomicrographs for quantification. Moreover, low-content target nucleic acids are difficult to detect with organic dyes.…”

GeoChip-Based Analysis of the Functional Gene Diversity and Metabolic Potential of Microbial Communities in Acid Mine Drainage

“…AMD is a prevalent, international environmental problem that threatens aquatic life and surrounding ecosystems (11,15,21,24,26,33,43,44), although it also has been regarded as an excellent model to investigate linkages between microbial communities and geochemistry because of its relatively simple microbial community composition and structure (5,6,8). A better understanding of the microbial community diversity and ecology in AMD would provide a scientific foundation not only for the bioremediation of AMD-contaminated environments but also for potential applications for the energy-efficient recovery of valuable metals from mine waste and the removal of sulfur from coal (6, 23).The phylogenetic diversity of AMD microbial communities has been well studied by 16S rRNA-based approaches, including PCR cloning (36,54,55,57), denaturing gradient gel electrophoresis (DGGE) (10,20,21), fluorescence in situ hybridization (9,29,40), and oligonucleotide microarrays (14, 56). For instance, the DNA sequencing of a natural acidophilic biofilm (pH 0.80) revealed that pathways for carbon and nitrogen fixation and energy generation might occur in these communities and provided insight into survival strategies in such an extreme environment (45).…”

“…The phylogenetic diversity of AMD microbial communities has been well studied by 16S rRNA-based approaches, including PCR cloning (36,54,55,57), denaturing gradient gel electrophoresis (DGGE) (10,20,21), fluorescence in situ hybridization (9,29,40), and oligonucleotide microarrays (14, 56). For instance, the DNA sequencing of a natural acidophilic biofilm (pH 0.80) revealed that pathways for carbon and nitrogen fixation and energy generation might occur in these communities and provided insight into survival strategies in such an extreme environment (45).…”

Occurrence and Evaluation of Chromium Reducing Bacteria in Seepage Water from Chromite Mine Quarries of Orissa, India

“…Reports on the analysis of microbial density and activity of the seepage water sampled from chromite mining areas are relatively very few compared to the extensive microbial studies of acid mine drainage [23,24]. Though the microbial activity of the samples was positively correlated with the total microbial counts, the population density of aerobic heterotrophic bacteria was relatively low ( Table 2) and the calculated r value far exceeds the tabulated one at P = 0.001.…”