Detection of a specific pattern of hyaluronan oligosaccharides and their binding proteins in human ovarian tumour

Abstract

Down regulation of hyaluronan (HA) at tumour epithelial cells results in the generation of O-HA. Increasing evidence about O-HA biological functions (mainly in vitro) are available, but we lack information of O-HA sizes generated during tumorigenesis and less information is available about their receptor interaction. We used biochemical approaches and identified that tumour cells of different grade possessing heterogeneity in generation of O-HA and specificity towards receptor binding. We identified a new receptor for HA 6-mer that over express in cancer tissue that shows its role in tumour progression. Collectively, simultaneous identification of HA sizes and their receptors could serve as a prognostic marker.

Tumor-Induced Cholesterol Efflux from Macrophages Drives IL-4 Mediated Reprogramming and Tumor Progression

“…Our in vitro studies suggest hyaluronic acid (HA) could be an important factor produced by cancer cells that promotes this process. HA is a major component of the extracellular matrix (ECM) in many human cancers, including ovarian cancer and in many cases the degree of HA accumulation strongly correlates with poor prognosis (Kolapalli et al, 2016;Sironen et al, 2011). Macrophages express at least two distinct receptors for HA; CD44 and Lyve1.…”

“…Several previous studies have shown that the extracellular matrix (ECM) component hyaluronic acid (HA) can be produced by tumor cells and has been linked with increased tumor progression (Chanmee et al, 2016). HA also forms high molecular weight oligomers (>100 kDa) with distinct biological activity (Gomez-Aristizabal et al, 2016;Kolapalli et al, 2016;Rayahin et al, 2015). Furthermore, receptors for HA are expressed by TAM, namely CD44 and Lyve-1 (Chanmee et al, 2016;Turley et al, 2002).…”

“…These experiments suggested that high molecular weight HA produced by ID8 cells promotes membrane cholesterol depletion in macrophages. Given that HA is an important component of ECM in many cancers, including EOC (Kolapalli et al, 2016), this suggests HA could affect the phenotype of TAM through membrane cholesterol depletion.…”