Detection of a single nucleotide polymorphism for insecticide resistance using recombinase polymerase amplification and lateral flow dipstick detection

Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates

“…Isothermal recombinase polymerase amplification (RPA) has also been exploited for the detection of SNPs, using allele-specific ligation to discriminate genetic variants related to cardiovascular diseases, which also has been used for the parallelized detection of the genotyping of four SNPs related to the treatment of tobacco addiction . A forward primer SNP detection approach using RPA has also been reported, where removing the reverse primer enhanced discrimination with single, double, and three scattered mismatches, and this approach was combined with lateral flow detection of an SNP that results in pyrethroid resistance in Aedes aegypti mosquitoes . Giant magnetoresistive (GMR) nanosensors using RPA have been applied to the genotyping of four SNPs (rs4633, rs4680, rs4818, and rs6269) along the catechol- O -methyltransferase gene ( COMT ) in salvia samples .…”

“… 42 A forward primer SNP detection approach using RPA has also been reported, where removing the reverse primer enhanced discrimination with single, double, and three scattered mismatches, and this approach was combined with lateral flow detection of an SNP that results in pyrethroid resistance in Aedes aegypti mosquitoes. 43 Giant magnetoresistive (GMR) nanosensors using RPA have been applied to the genotyping of four SNPs (rs4633, rs4680, rs4818, and rs6269) along the catechol- O -methyltransferase gene ( COMT ) in salvia samples. 44 Allelle-specific RPA has been used for the real-time fluorescence detection of the point mutation encoding hemoglobin S (HbS) in capillary blood, with the entire assay complete in <30 min at a cost of <$5.…”

OWL2: a molecular beacon-based nanostructure for highly selective detection of single-nucleotide variations in folded nucleic acids

“…20,21 Recent advances in SNV detection include ratio sensing via depletion of wild-type (WT) target, 22 programmable DNAzymes, 23 the use of CRISPR/Cas systems in conjunction with hybridization chain reactions, 24 and detection via lateral flow dipsticks after recombinase polymerase amplification with altered primers. 25 The best studied hybridization probes, however, all share the challenges of inefficient hybridization with RNA and DNA analytes folded in stable secondary structures, difficulty differentiating between wild-type (WT) and SNV-containing DNA at ambient temperatures, and their high synthetic cost. 20,21,26,27 Of the hybridization assays, the MB probe, a fluorophoreand quencher-labeled DNA harpin, has one of the most elegant designs (Fig.…”

RART-LAMP: One-Step Extraction-Free Method for Genotyping within 40 min

“…However, the polymerases used in RPA and LAMP lack 5′ to 3′ exonuclease activity, making it difficult to integrate TaqMan probes as used in PCR. To address this issue, RPA uses endonuclease IV (cutting nfo probe) 17 or exonuclease III (cutting exo probe) 18 to recognize and cleave the tetrahydrofuran (THF) residue of probe. However, the most commonly used real-time LAMP methods rely on measuring turbidity or fluorescence by intercalating dyes.…”