Detection of a Putative Novel <i>Wolinella</i> Species in Patients with Squamous Cell Carcinoma of the Esophagus

Bohr, Ulrich R. M.; Segal, I; Primus, Anett; Wex, Thomas; Hassan, H.; Ally, R.; Malfertheiner, Peter

doi:10.1111/j.1523-5378.2003.00186.x

Cited by 24 publications

(24 citation statements)

References 16 publications

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“…DNA sequencing and consecutive biocomputing were performed as previously described (1,3). RFLP analysis was performed using restriction enzymes BfaI and BstUI.…”

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confidence: 99%

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

et al. 2006

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Infections with enterohepatic Helicobacter species (EHS) can change the results of animal experiments.However, there is little information about the prevalence of EHS in noncommercial animal facilities. The aim of this study was to investigate the prevalence and the spread of EHS in specific-pathogen-free (SPF) mice. Fecal samples of 40 mouse lines were analyzed for members of the family Helicobacteraceae using a group-specific PCR targeting the 16S rRNA gene. Additional experiments were carried out to evaluate the spread of EHS among mice harbored in different caging systems. Helicobacter species were detected in 87.5% of the mouse lines tested. Five different Helicobacter species were identified: H. ganmani, H. hepaticus, H. typhlonicus, and the putative Helicobacter species represented by the isolates hamster B and MIT 98-5357. Helicobacter infection did not spread between animals in neighboring cages when individually ventilated cages were used; in contrast, when the mice were reared in open-air cages, EHS were found to spread from cage to cage. However, the spread was prevented by adding polycarbonate filter tops to the cages. When Helicobacter-negative and infected mice shared the same cage, transmission of the infection occurred in 100% within 2 weeks. Furthermore, we found that mice from commercial breeding facilities may carry undetected Helicobacter infections. Taken together, we show that infection with EHS may frequently occur and spread easily in mice reared under SPF conditions despite extensive safety precautions. Moreover, there is a high prevalence of rather uncommon Helicobacter species that may be a consequence of the current routine procedures used for health screening of SPF mice.

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“…DNA sequencing and consecutive biocomputing were performed as previously described (1,3). RFLP analysis was performed using restriction enzymes BfaI and BstUI.…”

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confidence: 99%

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

et al. 2006

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“…Biopsy specimens from the terminal ileum and the colon of each patient were analyzed by a group-specific PCR assay with primers C97 (5Ј-GCTATGACGGGTATCC-3Ј) and H2 (5Ј-TCGCAATGAGTATTCCTCTT-3Ј) as previously described (2,3). In order to identify the Helicobacter species, amplification products were sequenced as described before (3).…”

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confidence: 99%

“…In order to identify the Helicobacter species, amplification products were sequenced as described before (3).…”

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confidence: 99%

Identification of Enterohepatic Helicobacter Species in Patients Suffering from Inflammatory Bowel Disease

et al. 2004

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“…While such organisms (as previously stated) are morphologically consistent with the genus Helicobacter (26), it is surprising that given the large numbers of these organisms, a sequence similar to that of Wolinella was recovered. Despite the fact that only one species (W. succinogenes) has been described to date, another putative novel species (namely, "Candidatus W. africanus") was recently detected by PCR in patients with esophageal carcinoma (3). Given the substantial difference in nucleotide sequence (approximately 7 to 12%) from more than 40 Helicobacter and Campylobacter sequences, it is likely that this sequence represents a novel species.…”

Section: Discussionmentioning

confidence: 99%

“…Despite this limitation, its detection in the gut of this host, given the ability of other species to cause similar conditions of disease in other marine mammals (16,17), is novel and reflects the potential for multiple genera of the family Helicobacteraceae to infect this host. Coinfection of a host by closely related taxa has some precedence in the literature and has been reported for Campylobacter, Helicobacter, and Wolinella species (3,24). While any of these species may have been responsible for the development of gastritis in this animal, they also may have been mere bystanders (3), as species of Helicobacter, like those of Wolinella, may be nonpathogenic in their natural host (25,26).…”

Section: Discussionmentioning

confidence: 99%

Species of the Family Helicobacteraceae Detected in an Australian Sea Lion ( Neophoca cinerea ) with Chronic Gastritis

2004

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We describe the first case of gastritis in a male Australian sea lion (Neophoca cinerea) in which members of the family Helicobacteraceae, particularly the genus Wolinella, were detected. The sea lion exhibited clinical signs of gastrointestinal disease, including abdominal pain, lack of appetite, and lethargy. Examination of one ileal and five gastric biopsy specimens collected over a 10-year period revealed persistent fibrosis and/or superficial focal erosion and ulceration of the lamina propria. Spiral-shaped organisms 5 to 12 m long were observed in two of the gut biopsy specimens. While Helicobacter species were detected by PCR in one of the gastric biopsy specimens, Wolinella species were detected in four of the five gastric specimens, including those in which spiralshaped organisms were observed. Comparisons of biopsy specimen ribosomal DNA sequences with those obtained from the feces of this animal, the gastric tissue of a clinically healthy individual, and the feces of several other cohoused sea lions and fur seals revealed a separate and possibly novel gastric Helicobacter species. A possibly novel Wolinella species, along with Wolinella succinogenes, was also identified. These findings highlight the pathogenic potential of other members of this family in the etiopathogenesis of gastric disease in these animals.

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Detection of a Putative Novel Wolinella Species in Patients with Squamous Cell Carcinoma of the Esophagus

Abstract: Helicobacteraceae were detected in approximately 50% of South African patients with esophageal carcinoma. Furthermore, a novel bacterium was identified that might be linked to the enhanced incidence of esophagitis and subsequent malignant disease in South Africa.

Cited by 24 publications

References 16 publications

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

Prevalence and Spread of Enterohepatic Helicobacter Species in Mice Reared in a Specific-Pathogen-Free Animal Facility

Identification of Enterohepatic Helicobacter Species in Patients Suffering from Inflammatory Bowel Disease

Species of the Family Helicobacteraceae Detected in an Australian Sea Lion ( Neophoca cinerea ) with Chronic Gastritis

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