Species of the Family
            <i>Helicobacteraceae</i>
            Detected in an Australian Sea Lion (
            <i>Neophoca cinerea</i>
            ) with Chronic Gastritis

Oxley, Alan; Powell, Mark D.; McKay, David

doi:10.1128/jcm.42.8.3505-3512.2004

Cited by 20 publications

(14 citation statements)

References 29 publications

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“…aurati has been associated with chronic gastritis, intestinal metaplasia and one reported case of gastric adenocarcinoma in hamsters however role these organisms play in the etiology of these diseases requires further elucidation (Patterson et al 2000a, b;Nambiar et al 2005). The role of other gastric Helicobacter species in other animals such as H. cetorum in marine mammals and ''Candidatus H. bovis'' in ruminants is currently unknown though gastric ulcers are found in these animals (De Groote et al 1999b;Harper et al 2002a;Oxley et al 2004;Haesebrouck et al 2009). …”

Section: Disease Association In Animalsmentioning

confidence: 95%

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The Family Helicobacteraceae

et al. 2014

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Section: Disease Association In Animalsmentioning

confidence: 95%

“…W. succinogenes was detected in gastric biopsies from a sea lion with gastritis (Oxley et al 2004). In 2003, Bohr et al detected ''Candidatus Wolinella africanus'' DNA from esophageal mucosa biopsies of South African patients suffering from stenosing squamous cell carcinoma (Bohr et al 2003).…”

Section: Pathogenicity Clinical Relevancementioning

confidence: 98%

The Family Helicobacteraceae

et al. 2014

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“…However, complete genome sequencing of W. succinogenes has revealed that this organism shares 1,269 of its 2,046 genes with H. pylori and Campylobacter jejuni, many of which were identified as virulence factors (2). Interestingly, W. succinogenes was recently detected in gastric biopsies from a sea lion with gastritis (17). Our finding of W. succinogenes in the intestinal tract of children with gastrointestinal symptoms suggests that the status of this organism as a nonpathogen may need to be reevaluated.…”

Section: Fig 2 Fish Analysis Of Sections Cut From Biopsy Samples (A)mentioning

confidence: 99%

Nongastric Helicobacter Species Detected in the Intestinal Tract of Children

et al. 2006

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The presence of members of the family Helicobacteraceae in the colon of children was investigated using PCR, denaturing gradient gel electrophoresis, and fluorescent in situ hybridization. The rate of detection of species belonging to the Helicobacteraceae family in children with inflammatory bowel disease (92%) and irritable bowel syndrome (100%) was found to be significantly higher than that in healthy children (25%) (P < 0.05).Helicobacter species are gram-negative curved to spiralshaped bacteria that naturally colonize the mucus layer of the gastrointestinal tract of a range of mammalian hosts (15). Based on their preferential site of colonization, species of the Helicobacter genus have been divided into gastric and enterohepatic subgroups. Helicobacters in the gastric group, represented by Helicobacter pylori, have been associated with gastritis and other gastric disorders (15, 16). Enterohepatic Helicobacter species, which colonize the intestine and hepatobiliary system, have been linked to chronic hepatic and intestinal diseases (9, 16) as well as inflammatory bowel disease (IBD) in immunodeficient rodents (10, 13). The role of Helicobacter species in human IBD is currently controversial (4,5,19).In this study, we assessed the presence and spatial distribution of members of the Helicobacteraceae family (genera Helicobacter and Wolinella) in the colon of 21 children (16/21 male, aged 3 to 15 years) undergoing diagnostic colonoscopy and determined their association with colonic inflammation. The H. pylori status of all children, as assessed by histological examination and rapid urease testing of gastric biopsies, showed that 20/21 children were negative for H. pylori.Three colonic biopsies were collected from each child at colonoscopy. One biopsy was fixed in 10% neutral formalin for routine histological examination, the second biopsy was fixed in Carnoy's fixative for fluorescent rRNA in situ hybridization (FISH) analysis and Alcian blue-periodic acid-Schiff (PAS) staining, and the third biopsy was placed directly into 600 l of lysis buffer (Gentra Systems, Minneapolis, Minn.) for DNA extraction.DNA was extracted from freshly collected biopsy tissues using the PUREGENE DNA extraction kit (Gentra Systems, Minneapolis, Minn.). The16S rRNA gene of members of the family Helicobacteraceae was amplified by PCR using primers H276f and H676r with modified thermal cycle conditions (18). Hot-start PCRs were performed in a 25-l volume containing 10 pmol of each primer, 1ϫ PCR buffer (Fisher Biotech), 200 nM concentrations of each nucleotide triphosphate, 1.5 mM MgCl 2 , and 200 to 250 ng of extracted DNA. Thermal cycling conditions were 94°C for 5 min and 35 cycles of 94°C for 5 s, 57°C for 5 s, and 72°C for 30 s. Twenty-microliter PCR products were examined on a 1.5% agarose gel. PCR-denaturing gradient gel electrophoresis (DGGE) analysis was conducted according to a method described previously by Grehan et al. (11). Individual DGGE bands were excised and sequenced.Slides prepared from biopsies fixed in Carnoy's fi...

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“…This has led researchers to question the nonpathogenic status of W succinogenes. 38 However, the Wolinella spp identified in the dogs of the study reported here clustered more closely with W succinogenes than with Candidatus W africanus. 37 A new putative Wolinella sp has also been identified in the stomach of a sea lion with gastritis.…”

Section: Discussionmentioning

confidence: 54%