Detection of a porcine rotavirus strain with VP4, VP7 and NSP4 genes of different animal origins

Abstract

A new rotavirus strain, sh0902, was detected in diarrheic piglets on a farm in Shanghai, China, and its genotype was characterized as G1P[7]. Analysis of the VP4, VP7 and NSP4 genes demonstrated VP4 homology to bovine and swine rotavirus strains; the nucleotide (nt) and amino acid (aa) identities were 99.7% and 99.5%, respectively. The VP7 gene was highly homologous to that of a giant panda rotavirus strain, with 98.5% similarity at the nt level and 99% similarity at the aa level. The nucleotide sequence of the NSP4 gene displayed high homology to human rotavirus strain R479, with 99.7% identity at the nt level and 99.3% identity at the aa level. This is the first report of an unusual porcine rotavirus strain with VP4, VP7 and NSP4 genes that are highly homologous to bovine, swine, giant panda and human strains isolated at geographically distant sites (South Korea, China and India). Our data indicate that rotaviruses have circulated among humans and animals and undergone genome reassortment.

Exotic rotaviruses in animals and rotaviruses in exotic animals

“…Strain RU172 was hypothesized to be derived from porcine-human reassortment events, or alternatively, it was believed that the Wa-like human and porcine G12 strains might have originated from a common ancestor, and eventually evolved (by genetic drift and shift) with time [37]. The VP7 gene of a porcine G1P [7] RVA strain RVA/ Pig-wt/CHN/sh0902/2007/G1P [7] was found to be more closely related (nucleotide sequence identity of 98.5 %) to that of a giant panda RVA strain CH-1 and shared low nucleotide sequence identities (\90 %) with those of other G1 strains, whilst its VP4 and NSP4 genes of sh0902 were closely related to porcine or porcine-like RVA strains [148].…”

“…Humans R3 C3 M3 A3 N3 T3 E3 H3 Cattle The common bovine, canine/feline, equine, human and porcine RVA strains are italicised a The VP7 genes of strains sh0902 and CH-1 were most closely related to each other, and shared low nucleotide sequence identities (\ 90 %) with those of other G1 strains [46,148] b The porcine G2 VP7 gene was found to be genetically distinct from those of human G2 strains, and has been referred to as G2-like [16,17,61,71,75] c Only partial nucleotide sequence of the VP1 gene of strain KE4852/07 was obtained, which could not be assigned to any of the known VP1-R genotypes, and therefore, was tentatively designated as R8 [23] ''-'' indicates that no nucleotide sequence data were available in the GenBank database caprine G3P [3] strain, RVA/Goat-tc/KOR/GRV/1998/ G3P5 [3], provided evidence for reassortment events and/or interspecies transmission of canine, feline and/or simian RVAs [66].…”

Molecular epidemiological investigation of group A porcine rotavirus in East China

“…Although previous studies had investigated the prevalence rates of PoRV in several areas in China, very limited genotypes data of dominant PoRV isolates were available until now (21,26,27). The G/P genotype combinations of RVA identified mainly included G9P [7], G3P [13], G5P [13], G9P [13], G9P [23], G1P [7], and G9P [6] based on several isolate strains of publications during past decades in China (21,(28)(29)(30)(31)(32)(33). Considering the rare data of genotypes, the VP7 gene and VP4 gene from the nine RVA strains were sequenced to demonstrate the genotypic classifications in this study.…”