Detection of a novel recombinant strain of norovirus in an African-descendant community from the Amazon region of Brazil in 2008

Fumian, Túlio Machado; Aragão, Glicélia Cruz; Mascarenhas, Joana D’Arc Pereira; Kaiano, Jane Haruko Lima; Siqueira, Jones Anderson Monteiro; Soares, Luana da Silva; Linhares, Alexandre C.; Gabbay, Yvone Benchimol

doi:10.1007/s00705-012-1428-2

Cited by 14 publications

(17 citation statements)

References 23 publications

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“…This is in agreement with previous reports describing NoV recombinant strains, where the recombination breakpoints were estimated between nucleotides 4981 and 5117 (Bull et al, 2005;Fumian et al, 2012;Hansman et al, 2004;Hardy et al, 1997;Jiang et al, 1999;Katayama et al, 2002).…”

Section: Introductionsupporting

confidence: 93%

“…Complementary DNA (cDNA) was obtained using hexanucleotide random primers (Amersham Bioscience, UK) and the Superscript II™ reverse transcriptase (Invitrogen, USA) according to the manufacturer's instructions. In order to investigate the presence of putative recombination events within the analyzed strains, we performed a PCR targeting the overlapping region between ORF1 and ORF2 using primers (Mon431/432; G2SKR/G1SKR) and protocols previously described (Beuret et al, 2002;Fumian et al, 2012;Kojima et al, 2002). An amplicon of 564 bp was obtained and visualized by agarose gel electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic analyses of Norovirus strains detected in Uruguay reveal the circulation of the novel GII.P7/GII.6 recombinant variant

Fajardo

Tort

Victoria

et al. 2014

Infection, Genetics and Evolution

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Noroviruses (NoV) are one of the major etiological agent of acute gastroenteritis (AGE) outbreaks worldwide. Distinct NoV genotypes have been associated with different transmission patterns and disease severity in humans. Therefore, it is important to identify genetically different NoV genotypes circulating in a particular region. However, genotyping has become a challenge due to recombination events occurring mainly nearby ORF1/ORF2 junction of NoV genome, leading to distinct genotypes with polymerase and capsid regions derived from parenteral strains. Taking this into account, ORF1/ORF2 sequences were obtained from NoV strains collected from patients with AGE in Uruguay. This study reveals in silico evidences of recombination events taking place in four out of six strains analyzed for which its polymerase gene and its capsid region correspond to GII.P7 and to GII.6 genotype, respectively. These results also reveal the circulation of a GII.P7/GII.6 recombinant variant in the natural populations of NoV strains in the northwestern region of Uruguay. As far as we know this is the first report about the circulation of a NoV GII.P7/GII.6 recombinant variant in the Americas.

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Section: Introductionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Phylogenetic analyses of Norovirus strains detected in Uruguay reveal the circulation of the novel GII.P7/GII.6 recombinant variant

Fajardo

Tort

Victoria

et al. 2014

Infection, Genetics and Evolution

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“…NoV infection is known to occur in individuals of all ages due to its vast diversity of genotypes, its infectivity, the high rates of mutation and recombination which together lead to antigenic varieties and escape from the host immune system [29], [38], [39], [40], [41]. However, in our study, most cases of symptomatic NoV infection occurred among children up to 12 months old.…”

Section: Discussionmentioning

confidence: 53%

Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil

et al. 2013

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Noroviruses (NoV) are the main etiological agents of gastroenteritis outbreaks worldwide and susceptibility to NoV infection has been related to the histo-blood group antigen (HBGA). This study aimed to determine the prevalence of NoV strains and to evaluate the HBGA phenotype and genotype of children from semi-isolated Quilombola communities, descendents of black slaves in Brazil. A total of 397 children up to eleven years old, with and without diarrhea, from Quilombola Communities in the Espirito Santo State, Brazil, were investigated for the presence of NoV from August 2007 to September 2009. Feces were collected from all the children, and blood from the NoV positive children. NoV was screened by reverse transcription-PCR with primers for the RNA-dependent RNA polymerase region; genogroup was determined by PCR with primers for the C and D regions and genotyped by sequencing. HBGA phenotype was performed by gel-spinning and FUT2 and FUT3 were analyzed by PCR or sequencing analysis. NoV were detected in 9.2% (12/131) of diarrheic and 1.5% (4/266) of non-diarrheic children (p<0.05, Fisher’s exact test). GI and GII genogroups were present in 12.5% and 87.5% of the samples, respectively. The following genotypes were characterized: GII.4 (25%), GII.12 (25%), GII.6 (12.5%) and GI.1 (6.3%), GI.3 (12.5%) and GI.4 (6.3%). Children infected with NoV showed the A (n = 6), O (n = 6), and B (n = 2) HBGA phenotypes, and 13 of them were classified as secretors (Se) and one as a non secretor (se). Mutations of Se 40, 171,216,357,428,739,960 were found for the FUT2 gene and mutations of Le 59, 202, 314 for the FUT3 gene. The only se child was infected by NoV GI, whereas the Se children were indiscriminately infected by GI or GII. This study showed rates of NoV infection in symptomatic and asymptomatic Quilombola children consistent with other studies. However, children under 12 months were seven times more affected than those between 1 and 5 years old. GII.12 was as frequent as GII.4 and GI.1 and GI.4 were described for the first time in Brazil. Owing to the small number of cases studied, no clear pattern of susceptibility and/or HBGA resistance could be inferred.

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“…This study detected NoV in 19.2% of children hospitalized with acute gastroenteritis with an 8 annual range between 14.7% and 27.8% over the study period. Globally, hospital-based series in children with diarrhoea report a median of 14%, which seems lower than the proportions detected in our study [17].…”

Section: Discussionmentioning

confidence: 59%

“…Although NoV has become the most common cause of severe diarrhoea in industrialized countries with high rotavirus immunization [1,2], few studies have described NoV epidemiology since the introduction of rotavirus vaccines in other settings [5][6][7][8]. Brazil was one of the first countries to introduce a monovalent rotavirus vaccine (Rotarix) on a large scale in March 2006 [9], reaching high vaccine coverage levels (>80%) within a year of vaccine introduction and rapidly reducing the incidence of rotavirus-related hospitalizations [10,11].…”

Section: Introductionmentioning

confidence: 99%

Acute norovirus gastroenteritis in children in a highly rotavirus-vaccinated population in Northeast Brazil

Santos

Gurgel

Cavalcante

et al. 2017

Journal of Clinical Virology

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Running title: Norovirus diarrhoea in children in BrazilWord count: Abstract: 199. Manuscript: 2255. Highlights Gastroenteritis is one of the most important causes of morbidity and mortality in children.  Noroviruses are a leading cause of sporadic cases and outbreaks of acute gastroenteritis.  Norovirus annual detection rates increased over the study period.  The detection of norovirus was higher among young children. AbstractBackground: Gastroenteritis is one of the most important causes of morbidity and mortality

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Detection of a novel recombinant strain of norovirus in an African-descendant community from the Amazon region of Brazil in 2008

Cited by 14 publications

References 23 publications

Phylogenetic analyses of Norovirus strains detected in Uruguay reveal the circulation of the novel GII.P7/GII.6 recombinant variant

Phylogenetic analyses of Norovirus strains detected in Uruguay reveal the circulation of the novel GII.P7/GII.6 recombinant variant

Molecular Characterization of Noroviruses and HBGA from Infected Quilombola Children in Espirito Santo State, Brazil

Acute norovirus gastroenteritis in children in a highly rotavirus-vaccinated population in Northeast Brazil

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