Detection of a novel HLA‐B27 allele, B*2740, in Taiwanese volunteer bone marrow donors by sequence‐based typing: curiosity rewarded

Abstract

We report here a novel HLA-B allele, B*2740, discovered in Taiwanese volunteer marrow donors. The new sequence has nucleotide variation at position 527 (T-->A) as compared to B*2708. The nucleotide change caused an amino acid substitution from valine (V) to glutamic acid (E) at codon 152. Since B*2740 carries sequence confers to HLA-Bw6 public epitope we believe that this novel B*27 allele might have been generated from a gene conversion involving a Bw4-specific allele (probably B*2704) and a Bw6-specific allele.

Discovery of two novel HLA‐B alleles, B*46:13:03 and B*15:189, in two Taiwanese volunteer bone marrow donors by sequence‐based typing

“…Thus, the database of Taiwanese bone marrow donor registry consists of HLA alleles and haplotypes with its uniqueness. From time to time, new alleles, rare frequency alleles and Taiwanese‐conserved haplotypes were identified in our routine HLA typing practices (Chen et al. , 2010; Yang & Lin, 2007; Yang et al.…”

Rare HLA alleles and their predicted haplotypes in Tzu Chi Taiwanese marrow donor registry

“…B*27:40 was accidentally discovered by an attempt to validate its certainty using SBT method on all donors who were thought to carry B*27:08, an HLA‐B allele found in Caucasians. From our experience, we suggest oriental individuals who were previously tested to express B*27:08 by RELI‐SSO method should be re‐evaluated by SBT for its accuracy (Chen et al. , 2009c).…”

Using high-resolution human leukocyte antigen typing of 11,423 randomized unrelated individuals to determine allelic varieties, deduce probable human leukocyte antigen haplotypes, and observe linkage disequilibria between human leukocyte antigen-B and-C and human leukocyte antigen-DRB1 and-DQB1 alleles in the Taiwanese Chinese population

“…The DNA material was subjected to HLA genotyping for HLA-A,-B,-C,-DRB1, and-DQB1 loci using a commercial polymerase chain reaction- sequence-based typing (PCR-SBT) kit, the SeCore ® A/B/C/DRB1/DQB1 Locus Sequencing kit (Life Technologies, Brown Deer, WI, USA). High-resolution allelic sequencing was performed as previously described [1011]. …”