Rare HLA alleles and their predicted haplotypes in Tzu Chi Taiwanese marrow donor registry

Lin

et al. 2011

Self Cite

We report here an HLA-A allele, A*11:90, found in a Taiwanese cord blood sample using DNA sequence-based typing (SBT) protocol after observing an anomalous reaction pattern in a sequence-specific oligonucleotide (SSO) typing exercise. The sequence of A*11:90 is identical to A*11:01:01, the most predominant A*11 variant in Taiwanese, in exon 2 but differs from A*11:01:01 in exon 3 by two nucleotide substitutions at codon 163 (c.487C>G and c.488G>A), resulting R163E. In comparison with the sequence of A*11:02:01, the second most predominant subtype of A*11 in Taiwanese A*11:90 has one nucleotide difference at codon 19 (c.55A>G) in exon 2 resulting K19E and two nucleotides variations at codon 163 (c.487C>G and c.488G>A) in exon 3 resulting R163E. HLA-A*11:90-B*40:02-DRB1*11:01 is the deduced probable HLA haplotype in association with A*11:90. The generation of A*11:90 is thought to involve a DNA recombination event between alleles A*11:01:01 and A*80:01 where A*80:01 donated a fragment of the DNA sequence (from n.t. 487 to n.t. 497) to the recipient sequence of A*11:01:01.

Section: Resultsmentioning

confidence: 99%

Oriental HLA‐A11:90 detected in a Taiwanese cord blood sample and the haplotype in association with A11:90 allele

Lin

et al. 2011

Self Cite

“…From time to time, new alleles, rare frequency alleles and Taiwanese‐conserved haplotypes were identified in our routine HLA typing practices (Chen et al. , 2010; Yang & Lin, 2007; Yang et al. , 2011a,b).…”

Section: Resultsmentioning

confidence: 99%

“…The frequency of HLA‐B*15, ranked as the fourth most frequently observed allele in HLA‐B locus in Taiwanese, mainly composed of B*15:02 and B*15:01, while B*15:03, B*15:04, B*15:05, B*15:06, B*15:07, B*15:08, B*15:10, B*15:11, B*15:12, B*15:13, B*15:15, B*15:18, B*15:19, B*15:21, B*15:25, B*15:27, B*15:30, B*15:32, B*15:58; B*15:65 and B*15:109 representing the less observed alleles (Yang & Lin, 2007; Wen et al. , 2008; Yang et al. , 2009, 2011a,b; Lai et al.…”

Section: Introductionmentioning

confidence: 99%

Discovery of two novel HLA‐B alleles, B46:13:03 and B15:189, in two Taiwanese volunteer bone marrow donors by sequence‐based typing

Lin

et al. 2011

Self Cite

We report here two novel HLA-B alleles, B*46:13:03 and B*15:189, discovered in two Taiwanese volunteer bone marrow donors. The sequence of B*15:189 has a nucleotide sequence possibly derived from a recombination event between HLA-B*39:01:01 and B*15:01:01:01, while the origin of the sequence B*46:13:03 was less obvious to postulate, considering the low frequency of B*46:13 in the general population and the silent mutations involved. Our report here adds further HLA polymorphism to the growing lists of HLA-B*46 and HLA-B*15 and provides an additional HLA information for donor search programme for patients undergoing transplant.

“…The database of Taiwanese bone marrow donor registry in deed consists of HLA alleles and haplotypes with its unique polymorphism and characteristics (Lai et al. , 2010; Yang et al. , 2011).…”

Section: Introductionmentioning

confidence: 99%

Discovery of the novel HLA‐DRB110:04 allele in a Taiwanese volunteer bone marrow donor and identification of the probable HLA‐A, ‐B, ‐C and ‐DRB1 haplotype in association with DRB110:04

Kao

et al. 2012

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We report here a novel variant of HLA-DRB1*10, DRB1*10:04, discovered in a Taiwanese volunteer bone marrow donor by a sequence-based typing (SBT) method. The DNA sequence of DRB1*10:04 differs from DRB1*10:01:01, in exon 2, at nucleotide positions 296 (G fi A) and 303 (T fi G). The nucleotide changes caused an amino acid substitution at amino acid residue 70 (R fi Q). We hypothesize that the formation of DRB1*10:04 was probably the result of a gene recombination event where DRB1*10:01:01 received a minimum length of DNA sequence from DRB1*04:05:01, as the sequence of DRB1*10:04 is identical to DRB1*10:01:01 in exon 2 except the sequence from nucleotide 296 to nucleotide 303, which is identical to DRB1*04:05:01. The plausible HLA-A, -B, -C and - DRB1 haplotypes in association with DRB1*10:04 was deduced as A*01:01-B*37:01-C*06:02-DRB1*10:04.