Detection of a novel equine-like G3 rotavirus associated with acute gastroenteritis in Brazil

Abstract

Genotype G3P[8] of rotavirus A (RVA) is detected worldwide, usually associated with Wa-like constellation and exhibiting a long RNA migration pattern. More recently, a novel inter-genogroup, G3P[8] reassortant variant with a short electropherotype, has emerged in Asia, Oceania and Europe, denoting an overall potential of unusual rotavirus strains. During a RVA surveillance in Brazil, G3P[8] strains were found displaying a short electropherotype pattern, which had not been detected before in this region. This study aims to characterize the complete genome of 10 G3P[8] strains detected in the northern region of Brazil. All G3P[8] samples were subjected to partial sequencing, and the whole-genome phylogenetic analysis demonstrated that all strains possessed I2-R2-C2-M2-A2-N1-T2-E2-H2 genotype background, representing reassortants with an equine-like G3 VP7 and amino acid changes in VP4 and VP7 antigenic regions as compared to vaccine strains. Phylogenetic analysis demonstrated high nucleotide identity in almost all RNA segments of G3P[8] DS-1 samples detected in Asia, Oceania and Europe as well as G3P[4] strains in Japan. This study reports a novel, equine-like G3P[8] strain circulating in Brazil and isolated from children hospitalized for severe gastroenteritis, and highlights the complex dynamics of RVA molecular epidemiology. Our findings point to a novel RVA strain emerging in this region, and studies should be done to detect whether this may represent a challenge to current vaccine strategies.

High prevalence of equine‐like G3P[8] rotavirus in children and adults with acute gastroenteritis in Thailand

“…In the survey on the G/P type distributions in Thailand between 2000 and 2016, 4,6,7,37,43 various G/P type combinations of RVAs were observed: common G1P [8], G2P [4], G3P [8], and G9P [8], and uncommon G2P [8], G3P [3], G3P [9], G3P [10], G3P [19], G12P [6], and G12P [8] have been reported. G1P [8] was the most predominant RVA strain detected in Thailand, with an average frequency of detection of 49%, followed by G9P [8], G2P [4], and G3P [8], 24%, 14%, and 11%, respectively.…”

“…G3P[8] strains with a short RNA profile are described as DS‐1‐like (equine‐like) G3P[8] strains since their VP7 gene sequences are highly related to those of RVAs detected in horses . Recently, DS‐1‐like G1P[8] strains and equine‐like G3P[8] strains have spread worldwide …”

“…11,[13][14][15] Recently, DS-1-like G1P [8] strains and equine-like G3P [8] strains have spread worldwide. [8][9][10][11][12][14][15][16][17][18][19][20][21][22][23][24][25] In this study, we determined the G/P type distribution and carried out the full-genome characterization of two representative unusual equine-like G3P [8] strains which were highly prevalent (33.7%) in Thailand between January 2014 and September 2016. It is noteworthy that the equine-like G3P [8] strains with a short RNA profile that had been recently detected in several countries were predominant (65.3% to 89.8%) in various age groups across Thailand since 2015.…”

Prevalence of rotavirus and human bocavirus in immunosuppressed individuals after renal transplantation in the Northern Region of Brazil

“…The detection of RVA and HBoV in fecal samples of children with gastroenteritis has been described in several setting, including Brazil . The global prevalence of RVA and HBoV associated with gastrointestinal infection is approximately 40% and 6%, respectively .…”

“…The detection of RVA and HBoV in fecal samples of children with gastroenteritis has been described in several setting, including Brazil. [29][30][31] The global prevalence of RVA and HBoV associated with gastrointestinal infection is approximately 40% and 6%, respectively. 32,33 However, the findings that were discordant to that reported in other studies with solid organs receptors in Austria, where the frequency ranged from 0.8% to 1.5%.…”