Detection of 19-bp deletion within PLAG1 gene and its effect on growth traits in cattle

Xu, Wei; He, Hongbin; Li, Zheng; Xu, Jie; Lei, Chuzhao; Zhang, Guimin; Dang, Ruihua; Niu, Hongyu; Qi, Xinglei; Chen, Hong; Huang, Yongzhen

doi:10.1016/j.gene.2018.06.041

Cited by 34 publications

(29 citation statements)

References 26 publications

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“…Polymerase chain reaction (PCR) amplification was performed as previously reported (Jin et al, 2016;Li et al, 2017;Xu et al, 2018;Ju et al, 2020), and products were separated on 3.5 % agarose gels. PCR products were sequenced when each primer pair and had different genotypes (Yang et al, 2016).…”

Section: Indel Loci Detection and Dna Sequencingmentioning

confidence: 99%

Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle

Mao

Cheng

et al. 2020

Arch. Anim. Breed.

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Abstract. To speed up the progress of marker-assisted selection (MAS) in cattle breeding, the dual-specificity tyrosine phosphorylation-regulated kinase 2 (DYRK2), cadherin 2 (CDH2), and kinesin family member 1A (KIF1A) genes were chosen based on our pervious genome-wide association study (GWAS) analysis results. DYRK2 is a kinase that may participate in cell growth and/or development; it shows phosphorylation activity toward serine, threonine, and tyrosine fragments of proteins, and it is different from other protein kinases. The CDH2 gene encodes a classic cadherin, which is a member of the cadherin superfamily. The protein encoded by KIF1A is a member of the kinesin family and plays a role in the transportation of membrane organelles along axon microtubules. We detected insertion/deletion (InDel) variation in these three candidate genes in 438 individual cattle (Xinjiang Brown cattle and Wagyu × Luxi crossbreed cattle). Only DYRK2-P3-11 bp was polymorphic and genotyped. The polymorphism information content of DYRK2-P3-11 bp was 0.336. Correlation analyses showed that InDel polymorphism was significantly associated with six different milk traits. These findings may aid future analyses of InDel genotypes in cattle breeds, and speed up the progress of MAS in cattle breeding.

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Section: Indel Loci Detection and Dna Sequencingmentioning

confidence: 99%

Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle

Mao

Cheng

et al. 2020

Arch. Anim. Breed.

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“…Pleomorphic adenoma gene 1 (PLAG1), located on chromosome 8q12, belongs to the pleomorphic adenoma (PLA) gene family and encodes a zinc finger protein with 2 putative nuclear localization signals (7). PLAG1-associated Gene Ontology annotations include dNA binding transcription factor activity and transcriptional activator activity (8).…”

Section: Introductionmentioning

confidence: 99%

PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway

Huang

Feng

2020

Int J Mol Med

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Ovarian cancer (Oc) is one of the most lethal gynecological diseases. Novel prognostic biomarkers and therapeutic targets for Oc are urgently required. The aim of this study was to investigate the mechanisms that govern how pleomorphic adenoma gene 1 (PLAG1) influences the biological processes and chemosensitivity of Oc cells via the insulin-like growth factor-2 (IGF2) signaling pathway. differentially expressed genes in Oc were selected based on bioinformatics data. Oc and adjacent tissue specimen were collected, followed by the determination of the expression of PLAG1 and IGF2 signaling pathway-associated genes. The regulatory mechanisms of PLAG1 in Oc cells were analyzed following treatment with pcdNA or small interfering RNA (siRNA), and included the assessment of cell proliferation, migration, invasion and cisplatin resistance. PLAG1 was identified as an upregulated gene in OC. OC tissues exhibited increased expression of PLAG1 and IGF2 compared with the controls. Moreover, PLAG1 was observed to positively regulate the IGF2 signaling pathway. The siRNA-mediated silencing of PLAG1 resulted in decreased expression of IGF2, IGF1 receptor and insulin receptor substrate 1, as well as inhibited proliferation, migration, invasion and cisplatin resistance of Oc cells. Furthermore, the effect of PLAG1 was dependent on IGF2. PLAG1 may therefore be considered as a possible target for the treatment of Oc.

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“…Indel variants present the advantages of convenient detection and causing notable effects compared with other types of variations (such as SNPs, CNVs, and structural variations in the genome) [8,9]. Currently, indels are detected on an electrophoresis platform, which is an economical and fast method that does not require complicated experimental equipment [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Detection of rs665862918 (15-bp Indel) of the HIAT1 Gene and its Strong Genetic Effects on Growth Traits in Goats

Gao

Song

et al. 2020

Animals

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The hippocampus abundant transcript 1 (HIAT1) gene, which was detected by the genome-wide identification of selective sweeps among elite goat breeds and water buffalo, is proposed to play an important role in meat characteristics. Four indels of the HIAT1 gene selected from the NCBI and Ensembl databases were detected via a pooling and sequencing strategy. A 15 bp insertion (rs665862918) in the first intron of HIAT1 was selected and classified on an electrophoresis platform in the Shaanbei white cashmere goat (SBWC) population. The correlation analysis revealed that rs665862918 is significantly highly associated with chest width (p = 1.57 × 10−5), chest depth (p = 8.85 × 10−5), heart girth (p = 1.05 × 10−7), body length (p = 0.022), and height at hip cross (p = 0.023) in the SBWC population (n = 1013). Further analysis revealed that individuals with a genotype insertion/insertion (II) of the rs665862918 locus exhibited better growth trait performance than individuals with an insertion/deletion (ID) or deletion/deletion (DD). These findings verify that HIAT1 affects the body size of goats and that rs665862918 could be a potential molecular marker for growth traits in goat breeding.

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Detection of 19-bp deletion within PLAG1 gene and its effect on growth traits in cattle

Cited by 34 publications

References 26 publications

Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle

Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle

PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway

Detection of rs665862918 (15-bp Indel) of the HIAT1 Gene and its Strong Genetic Effects on Growth Traits in Goats

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Detection of 19-bp deletion within PLAG1 gene and its effect on growth traits in cattle

Cited by 34 publications

References 26 publications

Determination of genetic variation within the &lt;i&gt;DYRK2&lt;/i&gt; gene and its associations with milk traits in cattle

Determination of genetic variation within the &lt;i&gt;DYRK2&lt;/i&gt; gene and its associations with milk traits in cattle

PLAG1 silencing promotes cell chemosensitivity in ovarian cancer via the IGF2 signaling pathway

Detection of rs665862918 (15-bp Indel) of the HIAT1 Gene and its Strong Genetic Effects on Growth Traits in Goats

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Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle

Determination of genetic variation within the <i>DYRK2</i> gene and its associations with milk traits in cattle