Detection by Warburg manometry of compounds stimulatory to lactic acid bacteria

Abstract

The effect of aqueous algal extract, yeast extract casein peptone and meat peptone on acid production by 4 strains of lactic acid bacteria was tested manometrically using a milk-bicarbonate medium. A strain-dependent stimulation of the bacteria was detected after 4 h incubation. After fractionation of an aqueous extract of the green alga Scenedesmus obliquus on a Sephadex G25 column, 2 fractions with stimulatory activity were found using Streptococcus lactis as the test organism. One of the peaks of activity, confined to shortening of the lag phase, was due to hypoxanthine which, however, had only a slight stimulatory effect in conventional milk souring tests. The manometric method provides a sensitive and rapid test for detecting compounds with biological activity in /ig amounts, but it should be accompanied by milk souring tests for interpretation of the type of stimulation involved.

Supplementation of Spirulina platensis and Chlorella vulgaris Algae into Probiotic Fermented Milks

“…Microalgae, including C. vulgaris and S. platensis, can increase viability of probiotic bacteria (Varga and others 2002; Gyenis and others 2005; Molnár and others 2009). In accordance with previous reports by various authors (Shirota and others 1964;Stengel 1970;Zielke and others 1978;Kurita and others 1979;Webb 1982), the substances responsible for the stimulatory properties of this cyanobacterial biomass were identified as adenine, hypoxanthine, and free amino acids.…”

“…Therefore, they are known as therapeutic and functional food (Fox ; Dillon and Phan ; Parada and others ; Kreitlow and others ; De Caire and others ; Merchant and Andre ; Gyenis and others ). It seems that the co‐addition of microalgae and probiotics stimulates growth and increases viability and acid production of the probiotic bacteria (Shirota and others ; Stengel ; Zielke and others ; Kurita and others ; Webb ). On the other hand, microalgae present in fermented milks will affect the sensory properties of the final product.…”

In vitro Treatment with Intact Cells or Cell Lysates of Lactobacillus and Spirulina Induced Lowering Effects on Induced Hypercholesteremia

“…Therefore, they are known as therapeutic and functional food (Dillon and Phan, 1993). It seems that the co-addition of microalgae and probiotics stimulates growth and increases viability and acid production of the probiotic bacteria (Webb, 1982).…”