In vitro Treatment with Intact Cells or Cell Lysates of Lactobacillus and Spirulina Induced Lowering Effects on Induced Hypercholesteremia

Kamal, Shymaa; Hamouda, Ragaa A.; Mahrous, Hoda; Salem, Mohamed L.; Hamza, Hanafy A.; Elhafez, Ekbal Abd

doi:10.3923/ijp.2015.638.643

Cited by 8 publications

(3 citation statements)

References 24 publications

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“…The antitumor activity of L. acidophilus lysates has been also reported [ 30 ]. Moreover, cell lysates from probiotics including lactobacilli lower the cholesterol level, whereas the intact cells of these probiotics do not effectively reduce the cholesterol level in comparison with the cell lysates [ 31 ]. Although the beneficial effects of lysates from lactobacilli have been well documented, the health promoting properties, such as anti-adipogenic effect, of lysates from P. acidilactici have not been suggested.…”

Section: Resultsmentioning

confidence: 99%

Inhibitory Effect of Bacterial Lysates Extracted from Pediococcus acidilactici on the Differentiation of 3T3-L1 Pre-Adipocytes

Lee

Kang

2022

IJMS

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Postbiotics, including bacterial lysates, are considered alternatives to probiotics. The aim of the current study was to investigate the effect of bacterial lysates (BLs) extracted from Pediococcus acidilactici K10 (K10 BL) and P. acidilactici HW01 (HW01 BL) on the differentiation of 3T3-L1 pre-adipocytes. Both K10 and HW01 BLs significantly reduced the accumulation of lipid droplets and the amounts of cellular glycerides in 3T3-L1 cells (p < 0.05). However, another postbiotic molecule, peptidoglycan of P. acidilactici K10 and P. acidilactici HW01, moderately inhibited the accumulation of lipid droplets, whereas heat-killed P. acidilactici did not effectively inhibit the lipid accumulation. The mRNA and protein levels of the transcription factors, peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α, responsible for the differentiation of 3T3-L1 cells, were significantly inhibited by K10 BL and HW01 BL (p < 0.05). Both K10 and HW01 BLs decreased adipocyte-related molecules, adipocyte fatty acid-binding protein and lipoprotein lipase, at the mRNA and protein levels. Furthermore, both K10 and HW01 BLs also downregulated the mRNA expression of leptin, but not resistin. Taken together, these results suggest that P. acidilactici BLs mediate anti-adipogenic effects by inhibiting adipogenic-related transcription factors and their target molecules.

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Section: Resultsmentioning

confidence: 99%

Inhibitory Effect of Bacterial Lysates Extracted from Pediococcus acidilactici on the Differentiation of 3T3-L1 Pre-Adipocytes

Lee

Kang

2022

IJMS

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“…Several studies have indicated that Lactobacillus lysates ameliorate colitis by strengthening gut barrier function and modulating the mucosal inflammatory system [ 8 , 20 ]. Moreover, Lactobacillus lysates enhance antitumor immune responses [ 21 ] and improve hypercholesterolemia [ 22 ]. Furthermore, previous studies have demonstrated that L. plantarum ATG-K2 lysate prevents adipocyte differentiation in vitro [ 23 ].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effects of Lactobacillus plantarum HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition

Kim

Park

Lee

2022

IJMS

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Lactobacillus plantarum HAC01 has been shown to effectively treat metabolic diseases. However, the precise pharmacological effects and molecular mechanisms of L. plantarum HAC01 remain unclear. In this study, we investigate the anti-adipogenic effects of L. plantarum HAC01 lysate and its associated mechanism of action. To induce lipid accumulation, 3T3-L1 cells were incubated in differentiation media with or without L. plantarum HAC01 lysate. Our results show that L. plantarum HAC01 lysate treatment not only reduced lipid accumulation during the differentiation of 3T3-L1 cells, but also decreased the expression of adipogenic and lipogenic genes involved in lipid metabolism in a dose-dependent manner. Additionally, L. plantarum HAC01 lysate inhibited CCAAT/enhancer-binding protein (C/EBP) beta within 4 h of differentiation induction and inhibited peroxisome proliferator-activated receptor gamma, C/EBP alpha, and sterol regulatory element-binding proteins within 2 d. Moreover, treatment with L. plantarum HAC01 lysate increased the phosphorylation of adenosine monophosphate-activated protein kinase, an important regulator of energy metabolism, and decreased the phosphorylation of mitogen-activated protein kinase. These results indicate that L. plantarum HAC01 lysate may have anti-adipogenic effects and support its potential as a useful agent for the treatment of obesity.

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“…Phenolic compounds found in algae have various health-promoting functions in humans (Islam et al, 2003). Kamal et al (2015) studied the treatment with intact cells or cell lysates of Lactobacillus and Spirulina to stimulate lowering effect of hypercholesteremia. Prakash & Kumari (2011) were utilized Papaya pulp and Spirulina for preparation of low-fat and high-protein frozen yogurt.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Different Biological Activities of Spirulina platensis Extracts

El.Din

2019

Egypt. J. Bot.

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PIRULINA is aquatic and photosynthetic blue green algae and it is a good source of c-phycocyanin pigments. It is widely utilized as food supplement because it contains significant amount of proteins. This investigation is an effort to study some of medicinal applications of S. platensis such as antioxidant capacity, antimicrobial, cholesterol-reduction and anticancer. Antimicrobial activity of methanol, acetone, chloroform and petroleum ether extracts of dried S. platensis was assayed against two fungi (Aspergillus flavus and Aspergillus niger) and four bacterial species (Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhimurium). The S. platensis extracts exhibited significant activity against all tested bacteria and fungi. The highest inhibition zone (1.35 ±0.04cm) was observed in acetone extract against S. typhimurium. While, petroleum ether extract of S. platensis was recorded the minimum activities of all tested antimicrobial and the lowest inhibition zone against Staphylococcus aureus (0.52±0.01cm). The antioxidant potential of different solvent extracts clearly that acetone extract of S. platensis showed highest percentage of DPPH (2, 2 diphenyl-1picrylhydrazyl) scavenging activity (75%) and highest values of phenolic contents (0.562 mg/g DW). S. platensis displayed anticancer activity due to the productions of phycocyanin, allophycocyanin and other valuable products. Calf Serum was used to evaluate the potentiality of various S. platensis extracts to control the tumor effects in vitro. The cell proliferation assay showed that petroleum ether extract at a concentration of 125 µg ml-1 for 24hrs, significantly inhibited the growth of the Fetal Calf tumor cells (13% viability).

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In vitro Treatment with Intact Cells or Cell Lysates of Lactobacillus and Spirulina Induced Lowering Effects on Induced Hypercholesteremia

Cited by 8 publications

References 24 publications

Inhibitory Effect of Bacterial Lysates Extracted from Pediococcus acidilactici on the Differentiation of 3T3-L1 Pre-Adipocytes

Inhibitory Effect of Bacterial Lysates Extracted from Pediococcus acidilactici on the Differentiation of 3T3-L1 Pre-Adipocytes

Ameliorative Effects of Lactobacillus plantarum HAC01 Lysate on 3T3-L1 Adipocyte Differentiation via AMPK Activation and MAPK Inhibition

Evaluation of Different Biological Activities of Spirulina platensis Extracts

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