Detection by Radioligand Assay of Antibodies against Borna Disease Virus in Patients with Various Psychiatric Disorders

Abstract

Using a radioligand assay, which preserves the natural form of the antigen, antibodies against Borna disease virus nucleoprotein and phosphoprotein were detected in 11 and 19 sera of 171 psychiatric patients, respectively. Compared with results by Western blotting, three and nine sera were concordantly positive, respectively. The four sera showing the highest levels of antibodies by radioligand assay were all negative by Western blotting; however, dilution and inhibition tests supported the positive results. Our results suggest the importance of conformational structure to detect human anti-Borna disease virus antibodies.

Prevalence of Borna Disease Virus Antibodies in Healthy Japanese Black Cattle in Kyushu

“…1B and Table 1). As was the case in other studies [12,20], the reactivity of the sera to P antigen did not necessarily correlate between the ELISA and Western blotting assays in this study. The major difference between the two methods may be caused by the form of the antigen.…”

Detection of Antibodies against Borna Disease Virus Proteins in an Autistic Child and Her Mother

“…The secondary antibodies alone did not stain for vSPOTs; thus, the possibility of cross-reactivity was excluded. We then evaluated her serum for antibodies against recombinant N and P proteins by using WB and RLA, as described previously (6). Purified recombinant proteins were similarly resolved by using sodium dodecyl sulfatepolyacrylamide gel electrophoresis and subjected to WB.…”

“…Overcoming this situation is the first step of reevaluation of bornavirus surveys; therefore, we designed a diagnostic procedure for evaluating bornavirus epidemiology as follows: first, the presence of BDV-reactive antibodies is evaluated by using an indirect immunofluorescence assay (IFA), the most reliable assay for detecting these antibodies (2,5). Second, BDV-reactive samples are evaluated to confirm the presence of antibodies against linear and conformational BDV epitopes by using Western blotting (WB) and a radioligand assay (RLA), respectively (6). The presence of BDV-reactive antibodies should be confirmed for at least 2 BDV antigens to reduce the risk of false-positive results.…”

Two Neuropsychiatric Cases Seropositive for Bornavirus Improved by Ribavirin

“…All the patients examined for anti-bornavirus antibodies were those visiting the Department of Psychiatry, Osaka General Medical Center. The IgG, IgM and IgA titers against two bornavirus antigens, nucleoprotein and phosphoprotein, were assayed by radioligand assay as previously described (8,9). Blood samples were stored at 4°C after sampling, and the sera were divided into several tubes A c c e p t e d M a n u s c r i p t and stored at -80°C on the day of sampling.…”

“…We have previously detected bornavirus antibodies in humans using a highly sensitive and specific radioligand assay (8). Specifically, we detected either anti-bornavirus IgG, IgM, or IgA in approximately 20% of both Japanese psychiatric and control subjects (9).…”