Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces.

Pontes, LuÃ­s AndrÃ©; Dias‐Neto, Emmanuel; Rabello, Ana

doi:10.4269/ajtmh.2002.66.157

Cited by 187 publications

(180 citation statements)

References 20 publications

(19 reference statements)

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“…Molecular tools such as conventional PCR and improved DNA amplification methods have been shown capable of detecting schistosome DNA in a variety of samples. A highly repetitive, 121-base pair (bp) sequence has been used to detect DNA from S. mansoni and S. haematobium in stools, serum, urine, and plankton samples (Pontes et al 2002;Sandoval et al 2006).…”

Section: Discussionmentioning

confidence: 99%

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

et al. 2014

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The present study aimed to evaluate a polymerase chain reaction (PCR) assay used for detection of Schistosoma mansoni infection in Biomphalaria alexandrina snails in early prepatent period and to compare between it and the ordinary detection methods (shedding and crushing). Biomphalaria alexandrina snails are best known for their role as intermediate hosts of S. mansoni. DNA was extracted from infected snails in addition to noninfected ''negative control'' (to optimized the efficiency of PCR reaction) and subjected to PCR using primers specific to a partial sequence of S. mansoni fructose-1,6-bus phosphate aldolase (SMALDO). SMALDO gene was detected in the infected laboratory snails with 70, 85, and 100 % positivity at the 1st, 3rd, and 7th day of infection, respectively. In contrast, the ordinary method was not sensitive enough in detection of early prepatent infection even after 7 days of infection which showed only 25 % positivity. By comparing the sensitivity of the three methods, it was found that the average sensitivity of shedding method compared to PCR was 23.8 % and the average sensitivity of crushing method compared to PCR was 46.4 % while the sensitivity of PCR was 100 %. We conclude that PCR is superior to the conventional methods and can detect positive cases that were negative when examined by shedding or crushing methods. This can help in detection of the areas and times of high transmission which in turn will be very beneficial in planning of the exact timing of the proper control strategy.

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Section: Discussionmentioning

confidence: 99%

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

et al. 2014

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“…Pontes et al (2002) reported the first use of PCR for the diagnosis of Schistosoma mansoni DNA in faecal samples. High sensitivity and specificity rates were observed by this (Pontes et al 2003) and another group (Allam et al 2009) when compared to routine parasitological examination according to the Kato-Katz method (Katz et al 1972).…”

mentioning

confidence: 99%

Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Gomes

Marques

Enk

et al. 2009

Mem. Inst. Oswaldo Cruz

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“…The first study conducted with fecal samples and serum revealed that the conventional PCR can detect 2.4 fg of DNA of S. mansoni per gram of feces, which makes this method more sensitive than the KatoKatz method 15 .…”

Section: Discussionmentioning

confidence: 99%

“…Primers that amplify a 121 bp tandem repeat DNA fragment of the S. mansoni genome were used for the amplification of this region previously cited by PONTES et al 15 . Bovine Serum Albumin (BSA) (SIGMA, USA) was also added in order to neutralize the action of any inhibitors that could be present at the fecal samples 11 .…”

Section: Dna Extraction From Feces Artificially Marked With S Mansonmentioning

confidence: 99%

Two sequential PCR amplifications for detection of Schistosoma mansoni in stool samples with low parasite load

Espírito-Santo

Alvarado-Mora

Pintó³

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSchistosomiasis constitutes a major public health problem, with an estimated 200 million individuals infected worldwide and 700 million people living in risk areas. In Brazil there are areas of high, medium and low endemicity. Studies have shown that in endemic areas with a low prevalence of Schistosoma infection the sensitivity of parasitological methods is clearly reduced. Consequently diagnosis is often impeded due to the presence of false-negative results. The aim of this study is to present the PCR reamplification (Re-PCR) protocol for the detection of Schistosoma mansoni in samples with low parasite load (with less than 100 eggs per gram (epg) of feces). Three methods were used for the lysis of the envelopes of the S. mansoni eggs and two techniques of DNA extraction were carried out. Extracted DNA was quantified, and the results suggested that the extraction technique, which mixed glass beads with a guanidine isothiocyanate/phenol/chloroform (GT) solution, produced good results. PCR reamplification was conducted and detection sensitivity was found to be five eggs per 500 mg of artificially marked feces. The results achieved using these methods suggest that they are potentially viable for the detection of Schistosoma infection with low parasite load.

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Detection by polymerase chain reaction of Schistosoma mansoni DNA in human serum and feces.

Cited by 187 publications

References 20 publications

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

Molecular approach for detecting early prepatent Schistosoma mansoni infection in Biomphalaria alexandrina snail host

Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Two sequential PCR amplifications for detection of Schistosoma mansoni in stool samples with low parasite load

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