Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Gomes, Luciana I.; Marques, Letícia Helena Sousa; Enk, Martin Johannes; Coelho, Paulo Marcos Zech; Rabello, Ana

doi:10.1590/s0074-02762009000800021

Cited by 33 publications

(28 citation statements)

References 9 publications

(8 reference statements)

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“…PCR methods have shown high sensitivity and specificity for the detection of S. mansoni DNA in stool samples, yet PCR gave negative results among few cases considered sure positives by microscopy (tables1,2) . Similar results were obtained by many authors (13,25,28,29,30) . Table ( (30) .…”

Section: Fig 1 Map Showing Schistosomiasis Endemic Areassupporting

confidence: 92%

“…Similar results were obtained by many authors (13,25,28,29,30) . Table ( (30) . (25) reported that missed cases after PCR were certainly misdiagnosed by the DNA amplification assay due to many factors such as: inhibition of the amplification reaction by fecal compounds and ⁄ or DNA degradation during transportation from the field, variation in egg output and uneven distribution in feces.…”

Section: Fig 1 Map Showing Schistosomiasis Endemic Areassupporting

confidence: 92%

“…More recently, Gomes et al (2006) (30) ,Hung et al (2008) (32) and lier et al (2009) (34) established and developed SYBR Green ( a fluorescent based technology) and Taq Man probe real time PCR assays for the detection and quantification of schistosomal DNA. They reported that they are potentially useful for quantification of parasite burden in human infection.…”

Section: Fig 1 Map Showing Schistosomiasis Endemic Areasmentioning

confidence: 99%

See 2 more Smart Citations

Diagnosis of Schistosomiasis in Low Endemic Areas

Allam¹

2012

Schistosomiasis

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Section: Fig 1 Map Showing Schistosomiasis Endemic Areassupporting

confidence: 92%

Section: Fig 1 Map Showing Schistosomiasis Endemic Areassupporting

confidence: 92%

Section: Fig 1 Map Showing Schistosomiasis Endemic Areasmentioning

confidence: 99%

See 1 more Smart Citation

Diagnosis of Schistosomiasis in Low Endemic Areas

Allam¹

2012

Schistosomiasis

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“…Bovine Serum Albumin (BSA) (SIGMA, USA) was also added in order to neutralize the action of any inhibitors that could be present at the fecal samples 11 .…”

Section: Dna Extraction From Feces Artificially Marked With S Mansonmentioning

confidence: 99%

Two sequential PCR amplifications for detection of Schistosoma mansoni in stool samples with low parasite load

Espírito-Santo

Alvarado-Mora

Pintó³

et al. 2012

Rev. Inst. Med. trop. S. Paulo

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SUMMARYSchistosomiasis constitutes a major public health problem, with an estimated 200 million individuals infected worldwide and 700 million people living in risk areas. In Brazil there are areas of high, medium and low endemicity. Studies have shown that in endemic areas with a low prevalence of Schistosoma infection the sensitivity of parasitological methods is clearly reduced. Consequently diagnosis is often impeded due to the presence of false-negative results. The aim of this study is to present the PCR reamplification (Re-PCR) protocol for the detection of Schistosoma mansoni in samples with low parasite load (with less than 100 eggs per gram (epg) of feces). Three methods were used for the lysis of the envelopes of the S. mansoni eggs and two techniques of DNA extraction were carried out. Extracted DNA was quantified, and the results suggested that the extraction technique, which mixed glass beads with a guanidine isothiocyanate/phenol/chloroform (GT) solution, produced good results. PCR reamplification was conducted and detection sensitivity was found to be five eggs per 500 mg of artificially marked feces. The results achieved using these methods suggest that they are potentially viable for the detection of Schistosoma infection with low parasite load.

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“…Earlier studies have described PCR methods for detection of Schistosoma mansoni and Schistosoma haematobium DNA in animal models and in human samples. [12][13][14][15][16] Schistosoma japonicum PCR techniques have also been described. [17][18][19][20][21][22] However, the use of PCR techniques to screen field-collected samples has been limited.…”

Section: Introductionmentioning

confidence: 99%

Field Evaluation of a PCR Test for Schistosoma japonicum Egg Detection in Low-Prevalence Regions of China

Fung

Xiao²,

Wang³

et al. 2012

The American Journal of Tropical Medicine and Hygiene

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Abstract. Sensitive Schistosoma japonicum detection methods are needed to progress from schistosomiasis control to elimination. The sensitivity of the Kato-Katz thick smear and miracidium hatching tests decrease with infection intensity and serological tests cannot always identify current infections. We evaluated a fecal polymerase chain reaction (PCR) assay to detect S. japonicum infection in 106 humans and 8 bovines in China. PCR was highly sensitive, detecting S. japonicum DNA at 0.5 eggs/g of stool. Comparing PCR examination of a single stool sample to the miracidium hatching test using three consecutive stool samples, more humans were hatching test positive (20%) than PCR positive (15%). However, two individuals were PCR positive in a village where no infections were detected by coprological methods. The sensitivity of PCR makes it a promising tool for schistosomiasis diagnostics and screening, although egg shedding variability and stool sample size present challenges for any detection method in low-transmission areas.

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Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples

Cited by 33 publications

References 9 publications

Diagnosis of Schistosomiasis in Low Endemic Areas

Diagnosis of Schistosomiasis in Low Endemic Areas

Two sequential PCR amplifications for detection of Schistosoma mansoni in stool samples with low parasite load

Field Evaluation of a PCR Test for Schistosoma japonicum Egg Detection in Low-Prevalence Regions of China

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