Detection Bioactive Metabolites of Fructobacillus fructosus Strain HI-1 Isolated from Honey Bee’s Digestive Tract Against Paenibacillus larvae

Zeid, Azza Abou; Khattaby, A. M.; El-Khair, Ibrahim A Abou; Gouda, Hend I A

doi:10.1007/s12602-021-09812-5

Cited by 8 publications

(4 citation statements)

References 71 publications

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“…Fructobacillus fructosus , a member of the genus Fructobacillus, has been reported to exhibit antagonistic activity against the pathogen bacterium Paenibacillus larvae , the causal agent of American foulbrood [ 79 ]. However, this species is not known from the results of the LEfSe analysis in this study but represents a good topic for further research.…”

Section: Discussionmentioning

confidence: 99%

Influence of Probiotic Feed Supplement on Nosema spp. Infection Level and the Gut Microbiota of Adult Honeybees (Apis mellifera L.)

2023

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Honeybees’ gut microbiota can provide new valuable access into the pathogenesis-related factors included in infections. Hence, we researched the presence and comparison of gut microbiota groups in control and Nosema spp.-infected honeybee colonies through high-throughput sequencing of the 16S rRNA. As the newest approach in apiary management, we hypothesize that the EM® probiotic for bees could have an important role in therapeutic and immunomodulatory effects on honeybee colonies. The aim of this study was to estimate its impact on the gut microbiota composition of adult honeybees. The major genera were detected, where Lactobacillus was the most abundant genus, followed by Gilliamela, Snodgrassella, and Bifidobacterium. Inoculation with Nosema spp. spores made the relative proportions of Bifidobacterium lower, which was ameliorated by EM® for bees’ application. In addition, EM® for bee applied treatments suppressed the increase in the number of Nosema spp. spores. This result points out that continuous EM® for bees treatment shall change bees’ gut microbiome composition and mitigate the influence of Nosema spp. infection. Snodgrassella alvi was a major member of the honeybee gut microbiota and may be significantly increased by long-term treatment with EM® for bees. Toward these results, it is possible that EM® for bees treatment will protect honeybees from herbicide glyphosate negative effects in agricultural fields by improving microbiome and immune functions.

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Section: Discussionmentioning

confidence: 99%

Influence of Probiotic Feed Supplement on Nosema spp. Infection Level and the Gut Microbiota of Adult Honeybees (Apis mellifera L.)

2023

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“…In this work, FLAB and LAB strains were shown to have an antifungal activity against both B. cinerea and A. niger , two moulds responsible for several harvest and post-harvest contaminations of fruits and vegetables, thus suggesting the application of the honey isolates as potential biocontrol agents [ 57 ]. Recently, Zeid et al [ 58 ] reported that a F. fructosus strain, isolated from honey bee’s digestive tract, was able to antagonise Paenibacillus larvae , probably due to the synergistic activity of several identified bioactive compounds. In another recent study, a bacteriocin, named kunkecin A, was purified from A. kunkeei and shown to inhibit Melissococcus plutonius , the aetiological agent of European foulbrood, an important disease of honey bees [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Properties, Functional Characterisation and Application of Fructobacillus fructosus and Lactiplantibacillus plantarum Isolated from Artisanal Honey

Simone

Rocchetti

Gatta

et al. 2022

Probiotics & Antimicro. Prot.

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Honey is a valuable reservoir of lactic acid bacteria (LAB) and, particularly, of fructophilic LAB (FLAB), a relatively novel subgroup of LAB whose functional potential for human and food application has yet to be explored. In this study, FLAB and LAB strains have been isolated from honeys of different floral origins and selected for their broad antimicrobial activity against typical foodborne pathogenic bacteria and spoilage filamentous fungi. The best candidates, two strains belonging to the species Lactiplantibacillus plantarum and Fructobacillus fructosus, were submitted to partial characterisation of their cell free supernatants (CFS) in order to identify the secreted metabolites with antimicrobial activity. Besides, these strains were examined to assess some major functional features, including in vitro tolerance to the oro-gastrointestinal conditions, potential cytotoxicity against HT-29 cells, adhesion to human enterocyte-like cells and capability to stimulate macrophages. Moreover, when the tested strains were applied on table grapes artificially contaminated with pathogenic bacteria or filamentous fungi, they showed a good ability to antagonise the growth of undesired microbes, as well as to survive on the fruit surface at a concentration that is recommended to develop a probiotic effect. In conclusion, both LAB and FLAB honey-isolated strains characterised in this work exhibit functional properties that validate their potential use as biocontrol agents and for the design of novel functional foods. We reported antimicrobial activity, cytotoxic evaluation, probiotic properties and direct food application of a F. fructosus strain, improving the knowledge of this species, in particular, and on FLAB, more generally.

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“…In the meantime, quite a few autochthonous LAB strains isolated from honey bee related sources ( Lactobacilus spp., the main representatives of LAB in particular) also demonstrated in vitro anti- P. larvae activities, which included a Lactobacillus strain ( L. apis sp. nov.), isolated from the honey crop (stomach) and detected mainly in the digestive tracts of 3-day-old honey bees, foraging workers and honey bee drones ( Killer et al 2014 ), the potent lactic acid producers of L. plantarum and L. brevis ( Mudroňová et al 2011 ), three L. johnsonii strains (including L. johnsonii CRL1647) ( Audisio et al 2011 ) and a set of LAB isolated from the gut (from esophagus to rectum) of worker honey bees ( Kačániová et al 2018 , 2020 , Al-Ghamdi et al 2020 , Iorizzo et al 2020 , Bielik et al 2021 , Zeid et al 2022 , Iorizzo et al 2022 ), Enterococcus faecium EFD ( Dimov et al 2020 ) and Enterococcus durans EDD2 (Lactobacillales, Enterococcaceae) ( Gyurova et al 2021 ) isolated from freshly collected pollen granules. In addition, metabolites and peptides, produced by LAB in honey, endowed polyfloral honeys with anti- P. larvae activities ( Erler et al 2014 ).…”

Section: Beneficial Bacteria Exhibiting Probiotic Potential In Afb Co...mentioning

confidence: 99%

Beneficial bacteria as biocontrol agents for American foulbrood disease in honey bees (Apis mellifera)

Yang

2023

Journal of Insect Science

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American foulbrood (AFB) is a cosmopolitan bacterial disease that affects honey bee (Apis mellifera) larvae and causes great economic losses in apiculture. Currently, no satisfactory methods are available for AFB treatment mainly due to the difficulties to eradicate the tenacious spores produced by the etiological agent of AFB, Paenibacillus larvae (Bacillales, Paenibacillaceae). This present review focused on the beneficial bacteria that displayed antagonistic activities against P. larvae and demonstrated potential in AFB control. Emphases were placed on commensal bacteria (genus Bacillus and lactic acid bacteria in particular) in the alimentary tract of honey bees. The probiotic roles lactic acid bacteria play in combating the pathogenic P. larvae and the limitations referring to the application of these beneficial bacteria were addressed.

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Detection Bioactive Metabolites of Fructobacillus fructosus Strain HI-1 Isolated from Honey Bee’s Digestive Tract Against Paenibacillus larvae

Cited by 8 publications

References 71 publications

Influence of Probiotic Feed Supplement on Nosema spp. Infection Level and the Gut Microbiota of Adult Honeybees (Apis mellifera L.)

Influence of Probiotic Feed Supplement on Nosema spp. Infection Level and the Gut Microbiota of Adult Honeybees (Apis mellifera L.)

Antimicrobial Properties, Functional Characterisation and Application of Fructobacillus fructosus and Lactiplantibacillus plantarum Isolated from Artisanal Honey

Beneficial bacteria as biocontrol agents for American foulbrood disease in honey bees (Apis mellifera)

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