DETECTION AND QUANTIFICATION OF PHYTOCHEMICAL MARKERS OF<i>Ilex paraguariensis</i>BY LIQUID CHROMATOGRAPHY

Pinto, Rodrigo Moreira Caetano; Lemes, Bruna Mikulis; Zielinski, Acácio Antônio Ferreira; Klein, Traudi; Paula, Fernado de; Kist, Airton; Marques, Anna S. F.; Nogueira, Alessandro; Demiate, Ivo Mottin; Beltrame, Flávio Luís

doi:10.5935/0100-4042.20150117

Cited by 5 publications

(4 citation statements)

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“…All samples were run in duplicate and the peak compared with caffeine ( y = 07 x + 5E ‐ 13,109; r 2 = 1; range from 0.025 to 0.4 mg/ml), theobromine ( y = 07 x + 8E ‐ 875 707; r 2 = 0.9934; range from 0.05 to 0.4 mg/ml) and chlorogenic acid ( y = 07 x + 6E ‐ 35,898; r 2 = 0.9999; range from 0.025 to 0.4 mg/ml) standards ( Sigma Chemical Co ., USA). Calibration curve was obtained with the mentioned standards after dilution in the mobile phase and submitted to chromatographic separation . Contents of the caffeoyl derivatives were expressed in (5‐ O ‐caffeoylquinic) chlorogenic acid absorbance.…”

Section: Methodsmentioning

confidence: 99%

Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.‐Hil.) Elite Genetic Resources in a Germplasm Collection

Friedrich

Gonela

Gonçalves‐Vidigal

et al. 2017

Chemistry & Biodiversity

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The aim of this study was to evaluate the phytochemical and genetic diversity, relationships and identification of mate (Ilex paraguariensis A.St.-Hil.) elite genetic resources belonging to the Brazilian germplasm collection and mate breeding program. Mate has been studied due to the presence of phytochemical compounds, especially methylxanthines and phenolic compounds. The samples were collected from the leaves of 76 mate elite genetic resources (16 progenies × 5 localities). Total DNA was extracted from mate leaves and 20 random primers were used for DNA amplification. Methylxanthines (caffeine and theobromine) and phenolic compounds (chlorogenic, neochlorogenic, and criptochlorogenic acids) were quantified by HPLC. The genetic divergence estimated was higher within (92%) than among (8%) the different populations. Analysis of genetic distance between origins provided the formation of two groups by UPGMA cluster analysis, with higher polymorphism (94.9%). The average content of caffeine ranged from 0.01 to 1.38% and theobromine of 0.10 - 0.85% (w/w). The caffeoylquinic acids concentrations (1.43 - 5.38%) showed a gradient 3-CQA > 5-CQA > 4-CQA. The coefficient of genetic variation (CVg) was of low magnitude for all mono-caffeoylquinics acids. Significant correlations (positive and negative) were observed between the phytochemical compounds. Genetic diversity analysis performed by RAPD markers showed a greater intra-populational diversity; genetic resources with low caffeine and higher theobromine content were identified and can be used in breeding programs; the correlation between methylxanthines and phenolic compounds can be used as a good predictor in future studies.

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Section: Methodsmentioning

confidence: 99%

Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.‐Hil.) Elite Genetic Resources in a Germplasm Collection

Friedrich

Gonela

Gonçalves‐Vidigal

et al. 2017

Chemistry & Biodiversity

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“…Currently, NPs have multiple applications in human health, industry and environmental protection and they are anticipated to be involved in many more areas in the future. The large chemical structural diversity and consequently divergent biological activities of NPs has made them a prominent source for various pharmaceutical products, including antivirals [ 6 ], anti-Alzheimer compounds [ 7 ], immunosuppressives [ 8 ], antioxidants [ 9 ], antimicrobials [ 10 ], anti-inflammatory compounds [ 11 ], anti-adipogenic agents, digestive agents, and stimulants [ 12 ]. In the last decade, a decrease in NP discovery has caused a dramatic decline in discovery of new drugs [ 2 ].…”

Section: Natural Productsmentioning

confidence: 99%

Emerging biosensors in detection of natural products

Piroozmand

Mohammadipanah

Faridbod

2020

Synthetic and Systems Biotechnology

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Natural products (NPs) are a valuable source in the food, pharmaceutical, agricultural, environmental, and many other industrial sectors. Their beneficial properties along with their potential toxicities make the detection, determination or quantification of NPs essential for their application. The advanced instrumental methods require time-consuming sample preparation and analysis. In contrast, biosensors allow rapid detection of NPs, especially in complex media, and are the preferred choice of detection when speed and high throughput are intended. Here, we review diverse biosensors reported for the detection of NPs. The emerging approaches for improving the efficiency of biosensors, such as microfluidics, nanotechnology, and magnetic beads, are also discussed. The simultaneous use of two detection techniques is suggested as a robust strategy for precise detection of a specific NP with structural complexity in complicated matrices. The parallel detection of a variety of NPs structures or biological activities in a mixture of extract in a single detection phase is among the anticipated future advancements in this field which can be achieved using multisystem biosensors applying multiple flow cells, sensing elements, and detection mechanisms on miniaturized folded chips.

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“…The total amount of methylxanthine in 25 samples of Yerba Mate ranged from 3.69 to 12.7 mg/g, with concentrations of caffeine and theobromine as 0.001 to 10.1 and 0.02 to 5.03 mg/g, respectively [ 31 ]. To quantify theobromine and caffeine in I. paraguariensis extracts, quality by design (QbD) models and UHPLC were optimized and applied, and indicated good future potential for application of this methodology [ 32 ]. In another study of samples of Yerba Mate methylxanthines were quantified by HPLC–DAD, with caffeine consistently higher in content than theobromine.…”

Section: Bioactive Compoundsmentioning

confidence: 99%

Health Benefits of Bioactive Compounds from the Genus Ilex, a Source of Traditional Caffeinated Beverages

et al. 2018

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Tea and coffee are caffeinated beverages commonly consumed around the world in daily life. Tea from Camellia sinensis is widely available and is a good source of caffeine and other bioactive compounds (e.g., polyphenols and carotenoids). Other tea-like beverages, such as those from the genus Ilex, the large-leaved Kudingcha (Ilex latifolia Thunb and Ilex kudingcha C.J. Tseng), Yerba Mate (Ilex paraguariensis A. St.-Hil), Yaupon Holly (Ilex vomitoria), and Guayusa (Ilex guayusa Loes) are also traditional drinks, with lesser overall usage, but have attracted much recent attention and have been subjected to further study. This review summarizes the distribution, composition, and health benefits of caffeinated beverages from the genus Ilex. Plants of this genus mainly contain polyphenols and alkaloids, and show diverse health benefits, which, as well as supporting their further popularization as beverages, may also lead to potential applications in the pharmaceutical or nutraceutical industries.

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DETECTION AND QUANTIFICATION OF PHYTOCHEMICAL MARKERS OFIlex paraguariensisBY LIQUID CHROMATOGRAPHY

Cited by 5 publications

References 0 publications

Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.‐Hil.) Elite Genetic Resources in a Germplasm Collection

Genetic and Phytochemical Analysis to Evaluate the Diversity and Relationships of Mate (Ilex paraguariensis A.St.‐Hil.) Elite Genetic Resources in a Germplasm Collection

Emerging biosensors in detection of natural products

Health Benefits of Bioactive Compounds from the Genus Ilex, a Source of Traditional Caffeinated Beverages

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