Detection and possible elimination of the first recorded population of the zebra mussel (Dreissena polymorpha) in Portugal from a reservoir

Catita, David; Gama, Mafalda; Azedo, R.; Banha, Filipe; Pinto, João; Ilhéu, Ana; Anastácio, Pedro M.

doi:10.3391/mbi.2020.11.3.04

Cited by 5 publications

(5 citation statements)

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“…Moreover, D . polymorpha is considered largely absent from Portuguese waters, with a single detection from a small reservoir in southern Portugal in 2019 (Catita et al, 2020). Apart from C .…”

Section: Methodsmentioning

confidence: 99%

“… c A population was discovered in 2019 in southern Portugal and is believed to have been eradicated (Catita et al, 2020). …”

Section: Methodsmentioning

confidence: 99%

“…Annual monitoring of these reservoirs using conventional morphological methods (detection of larvae), have so far not detected D. polymorpha. Moreover, D. polymorpha is considered largely absent from Portuguese waters, with a single detection from a small reservoir in southern Portugal in 2019 (Catita et al, 2020). Apart from C. fluminea, which is also invasive in mainland Portugal (Rosa et al, 2011), none of the species known to be present at the Italian lakes are known to be present at the Portuguese study sites, and many of the species are altogether absent from Portugal (Table 1).…”

Section: Study Area and Target Speciesmentioning

confidence: 99%

“…of morphological taxonomic identification or intensive survey methodologies. For instance, the detection of D. polymorpha in southern Portugal(Catita et al, 2020) required the deployment of monitoring ropes in several water bodies. This approach is logistically challenging and has a high turnaround time between sample collection (i.e., rope deployment and consequently colonization) and species detection.…”

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confidence: 99%

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Speeding up the detection of invasive bivalve species using environmental DNA: A Nanopore and Illumina sequencing comparison

Egeter

Veríssimo

Lopes‐Lima

et al. 2022

Molecular Ecology Resources

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Traditional detection of aquatic invasive species via morphological identification is often time-consuming and can require a high level of taxonomic expertise, leading to delayed mitigation responses. Environmental DNA (eDNA) detection approaches of multiple species using Illumina-based sequencing technology have been used to overcome these hindrances, but sample processing is often lengthy. More recently, portable nanopore sequencing technology has become available, which has the potential to make molecular detection of invasive species more widely accessible and substantially decrease sample turnaround times. However, nanopore-sequenced reads have a much higher error rate than those produced by Illumina platforms, which has so far hindered the adoption of this technology. We provide a detailed laboratory protocol and bioinformatic tools (msi package) to increase the reliability of nanopore sequencing to detect invasive species, and we test its application using invasive bivalves while comparing it with Illumina-based sequencing. We sampled water from sites with preexisting bivalve occurrence and abundance data, and contrasting bivalve communities, in Italy and Portugal. Samples were extracted, amplified, and sequenced by the two platforms. The mean agreement between sequencing methods was 69% and the difference between methods was nonsignificant. The lack of detections of some species at some sites could be explained by their known low abundances. This is the first reported use of MinION to detect aquatic invasive species from eDNA samples.

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Section: Methodsmentioning

confidence: 99%

“… c A population was discovered in 2019 in southern Portugal and is believed to have been eradicated (Catita et al, 2020). …”

Section: Methodsmentioning

confidence: 99%

Section: Study Area and Target Speciesmentioning

confidence: 99%

mentioning

confidence: 99%

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Speeding up the detection of invasive bivalve species using environmental DNA: A Nanopore and Illumina sequencing comparison

Egeter

Veríssimo

Lopes‐Lima

et al. 2022

Molecular Ecology Resources

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“…Zebra mussels (Dreissena polymorpha) are causing extensive damage to hydraulic infrastructure, as they reproduce inside water conduits and attach to many different types of surfaces. Severe problems have been reported in fluvial systems and lakes (Aldridge et al, 2004;Wimbush et al, 2009;Nakano and Strayer, 2014;Olson et al, 2018;Morales et al, 2019;Catita et al, 2020), but also in pressurized irrigation networks (Araujo et al, 2006;Morales-Hernández et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Normalized pressure: a key variable to assess zebra mussel infestation in pressurized irrigation networks

Morales-Hernändez

Playán

Latorre

et al. 2022

Agricultural Water Management

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What we know and don’t know about the invasive zebra (Dreissena polymorpha) and quagga (Dreissena rostriformis bugensis) mussels

Karatayev

Burlakova

2022

Hydrobiologia

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Detection and possible elimination of the first recorded population of the zebra mussel (Dreissena polymorpha) in Portugal from a reservoir

Cited by 5 publications

References 0 publications

Speeding up the detection of invasive bivalve species using environmental DNA: A Nanopore and Illumina sequencing comparison

Speeding up the detection of invasive bivalve species using environmental DNA: A Nanopore and Illumina sequencing comparison

Normalized pressure: a key variable to assess zebra mussel infestation in pressurized irrigation networks

What we know and don’t know about the invasive zebra (Dreissena polymorpha) and quagga (Dreissena rostriformis bugensis) mussels

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