Detection and Molecular Characterization of a Phytoplasma Associated with Big Bud Disease of Tomatoes in Jordan

Anfoka, Ghandi; Khalil, Amjad; Fattash, Isam

doi:10.1046/j.1439-0434.2003.00709.x

Cited by 33 publications

(28 citation statements)

References 12 publications

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“…Six different taxonomic phytoplasma groups infect tomato plants wordwide (Blancard 2009;Davis et al 1997;Shaw et al 1993;Hiruki and Wang 2004;Anfoka et al 2003;Choueiri et al 2007;Garnier 2000;Lee et al 1993;Arocha et al 2007; . Eggplant diseases have also been reported to be associated with phytoplasmas of different groups ; Schneider et al 1995b;Sertkaya et al 2007;Kelly et al 2009;Al-Subhi et al 2011;Amaral-Mello et al 2010).…”

Section: Sequences and Phylogenetic Analysismentioning

confidence: 99%

Occurrence and incidence of phytoplasmas of the 16SrII-D subgroup on solanaceous and cucurbit crops in Egypt

Omar

Foissac

2011

Eur J Plant Pathol

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Symptoms reminiscent of phytoplasma infection were observed in four provinces (governorates) of Egypt in fields of eggplants, tomato plants and squash. Diseased plants exhibited stunting, leaf yellows and flower development abnormalities. PCR amplification of 16SrDNA with phytoplasma-specific primer pairs confirmed the phytoplasma presence. Sequencing and phylogenetic analysis indicated that all phytoplasmas had the same partial 16SrDNA sequence, assigning them to the 16SrII-D phytoplasma subgroup. Disease incidence was about 1% among the 20 squash fields surveyed and equally varied from 4% to 15% in the 20 eggplant fields and in the 40 tomato fields inspected. The widespread distribution of this phytoplasma in annual solanaceous and cucurbit crops suggests a wider plant host range including wild plants that could act as reservoir and insist on the need for a insect vector survey. A finer genetic differentiation of Egyptian 16SrII-D phytoplasma strains from different geographical origins and different host plants should help to better trace such epidemics.

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Section: Sequences and Phylogenetic Analysismentioning

confidence: 99%

Occurrence and incidence of phytoplasmas of the 16SrII-D subgroup on solanaceous and cucurbit crops in Egypt

Omar

Foissac

2011

Eur J Plant Pathol

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“…CP is now taxonomically described as 'Candidatus Phytoplasma trifolii' (Hiruki and Wang 2004). This phytoplasma was recently associated with diseases of pepper in Spain and tomato plants in Jordan (Anfoka et al 2003;Castro and Romero 2002).…”

mentioning

confidence: 99%

“…Finally, our data are in agreement with the presence of 'Ca. P. trifolii' in the region after its detection in Jordan (Anfoka et al 2003). …”

mentioning

confidence: 99%

Occurrence and distribution of ‘Candidatus Phytoplasma trifolii’ associated with diseases of solanaceous crops in Lebanon

et al. 2007

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A survey for phytoplasma diseases in tomato and pepper fields in Lebanon was conducted during 2003 and 2004. Tomato plants with stunting, yellowing or purplish leaves, proliferation of laterals buds, hypertrophic calyxes and virescent flowers were found in 25% of the tomato fields surveyed, where they represented 2-8% of the plants. Pepper plants displaying stunting and yellowing of leaves, were found in 27% of the fields and 1-4% of the plants were affected. Phytoplasmas infecting tomato and pepper had identical 16S-rDNA RFLP profiles and sequences. A phytoplasma isolate named PTL was transmitted by dodder from a diseased tomato plant to a periwinkle (Catharanthus roseus) plant in which it induced leaf yellowing, virescence and phyllody. 16S-rDNA phylogenetic analysis classified PTL as a strain of 'Candidatus Phytoplasma trifolii'.

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“…Among solanaceous plants, this has also been shown for tomato. Molecular identification has indicated that phytoplasmas associated with tomato big bud are affiliated to distinct groups, reported in various countries: group 16SrI in the United States (Lee et al, 1998), 16SrIII in Brazil (Amaral Mello et al, 2006a), 16SrVI in Jordan (Anfoka et al, 2003) and 16SrI, 16SrIII, 16SrV and 16SrXII in Italy (Del Serrone et al, 2001). Interestingly, the nucleotide sequence of phytoplasma associated with tomato big bud in Brazil (Amaral Mello et al, 2006a) showed a similarity coefficient of 1.0 with the sequence of strain EB01-Br01 from eggplant.…”

Section: A P O Amaral Mello and Othersmentioning

confidence: 99%

Identification by computer-simulated RFLP of phytoplasmas associated with eggplant giant calyx representative of two subgroups, a lineage of 16SrIII-J and the new subgroup 16SrIII-U

Mello

Eckstein

Flôres

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Symptoms resembling giant calyx, a graft-transmissible disease, were observed on 1-5 % of eggplant (aubergine; Solanum melongena L.) plants in production fields in Sao Paulo state, Brazil. Phytoplasmas were detected in 12 of 12 samples from symptomatic plants that were analysed by a nested PCR assay employing 16S rRNA gene primers R16mF2/R16mR1 followed by R16F2n/ R16R2. RFLP analysis of the resulting rRNA gene products (1.2 kb) indicated that all plants contained similar phytoplasmas, each closely resembling strains previously classified as members of RFLP group 16SrIII (X-disease group). Virtual RFLP and phylogenetic analyses of sequences derived from PCR products identified phytoplasmas infecting eggplant crops grown in Piracicaba as a lineage of the subgroup 16SrIII-J, whereas phytoplasmas detected in plants grown in Braganç a Paulista were tentatively classified as members of a novel subgroup 16SrIII-U. These findings confirm eggplant as a new host of group 16SrIII-J phytoplasmas and extend the known diversity of strains belonging to this group in Brazil. INTRODUCTIONEggplant (aubergine; Solanum melongena L.) is among the most important edible crop species grown in Brazil. Sao Paulo state is a leader in the production of this crop, where it is currently cultivated over 1037 ha, with a total annual yield of 47 549 tonnes (figures for 2009). A disease of low incidence affecting approximately 1-5 % of plants was recently observed in fields of eggplant grown in Bragança Paulista and Piracicaba. Symptoms of affected plants included foliar chlorosis, shoot proliferation, shortened internodes, stunting, enlarged calyces, small flowers and fruit of reduced size. Collectively, these symptoms closely resembled those described previously for giant calyx, a disease of eggplant first reported to occur in Brazil during the 1990s (Boiteaux et al., 1994). The latter disease was suspected to have a phytoplasma aetiology based on consistent observation by electron microscopy of phytoplasma cells in the phloem sieve elements of diseased plants. A subsequent investigation of DNA extracts derived from diseased eggplants in Sao Paulo state and Brasília-DF by PCR-RFLP analysis concluded that phytoplasmas associated with eggplant giant calyx were most similar to strains previously classified as members of 16Sr RFLP group 16SrIII, subgroup B (16SrIII-B; Barros et al., 1998).Eggplant diseases associated with phytoplasmas have been reported in other countries. In Japan, a phytoplasma affiliated to group 16SrI was characterized molecularly from eggplant with dwarfing symptoms (Okuda et al., 1997;Lee et al., 1998). A disease called little leaf was reported in Bangladesh and molecular identification indicated that the phytoplasma belonged to group 16SrVI (Siddique et al., 2001). The study also demonstrated the phytoplasma to be indistinguishable from brinjal little leaf (BLL) phytoplasma characterized previously in India (Lee et al., 1998; Seemüller et al., 1998). Recently, the BLL strain was classified as a member of group 16SrVI, subg...

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Detection and Molecular Characterization of a Phytoplasma Associated with Big Bud Disease of Tomatoes in Jordan

Cited by 33 publications

References 12 publications

Occurrence and incidence of phytoplasmas of the 16SrII-D subgroup on solanaceous and cucurbit crops in Egypt

Occurrence and incidence of phytoplasmas of the 16SrII-D subgroup on solanaceous and cucurbit crops in Egypt

Occurrence and distribution of ‘Candidatus Phytoplasma trifolii’ associated with diseases of solanaceous crops in Lebanon

Identification by computer-simulated RFLP of phytoplasmas associated with eggplant giant calyx representative of two subgroups, a lineage of 16SrIII-J and the new subgroup 16SrIII-U

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