Detection and Molecular Characterization of Porcine Enteric Calicivirus in Korea, Genetically Related to Sapoviruses

Kim, H. J.; Cho, H. S.; Cho, K. O.; Park, N. Y.

doi:10.1111/j.1439-0450.2006.00939.x

Cited by 41 publications

(28 citation statements)

References 13 publications

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“…However, the relatively low prevalence of porcine SaVs probably are due to the low sensitivity of their RT-PCR assays using broadly reactive primers as we discussed in the diagnosis section. Kim et al in South Korea [55] detected 4.9% Cowden SaV-positives from nursing and weaned piglets out of 102 diarrheic pig fecal samples using 2 pairs of Cowden-specific primers targeting the RdRp and the capsid region, respectively [19]. The low prevalence in this study may be due to the relatively lower sensitivity of the RdRp primers PEC46/45 used compared to the primers PEC66/65 [68] and the high specificity of the capsid primers they used.…”

Section: Prevalence Of Porcine Savsmentioning

confidence: 53%

“…Presently, porcine SaVs have been reported from the US, Venezuela, South Korea and Japan [44,45,50,54,55]. Most strains are Cowden-like (GIII) SaVs based on sequence in the capsid or RdRp region except for QW19/02/US, JJ681/00/US, LL26/02/US and K7/JP strains.…”

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

“…Two more prevalence studies of porcine enteric caliciviruses by RT-PCR were reported recently [54,55]. Martinez et al [54] detected 36 porcine enteric calicivirus-positives out of 204 (18%) fecal samples from piglets (0-9 weeks of age with or without diarrhea) in Venezuela using NoV-and SaV-conserved primers.…”

Section: Prevalence Of Porcine Savsmentioning

confidence: 99%

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Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Wang

Costantini

Saif

2007

Vaccine

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Porcine enteric caliciviruses include sapoviruses and noroviruses. Porcine sapoviruses infect pigs of all ages and cause diarrhea in young pigs, whereas porcine noroviruses were detected exclusively from adult pigs without clinical signs. Importantly, certain porcine norovirus strains were genetically and antigenically related to human noroviruses. This raises public health concerns that pigs may be reservoirs for emergence of epidemic human norovirus strains. This article reviews the discovery of porcine noroviruses and sapoviruses, their classification, diagnosis, epidemiology and genetic and antigenic relatedness to human caliciviruses.

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Section: Prevalence Of Porcine Savsmentioning

confidence: 53%

Section: Genetic Classification Of Porcine Savsmentioning

confidence: 99%

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Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Wang

Costantini

Saif

2007

Vaccine

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“…Serogroups of rotavirus have been detected isolately and as mixed infections in pigs with and without clinical signs of enteric disorder (Alfieri et al 1999, Barry et al 2008. Similar situations have been reported on porcine enteric caliciviruses (Kim et al 2006, Martinéz et al 2006. Again, the participation of these agents in association or not with PCV2 in some cases described here can not be ruled out.…”

Section: Discussionmentioning

confidence: 66%

Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome

et al. 2008

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RESUMO.-[Lesões entéricas em suínos afetados por síndrome multissistêmica do definhamento dos suínos.]Amostras de linfonodos mesentéricos e intestinos de 79 leitões desmamados refugos, entre 3 e 5 meses de idade e confirmados como naturalmente afetados pela síndrome multissistêmica do definhamento foram estudadas. Os suí-nos eram oriundos de 12 criações no sul do país e foram selecionados em função dos sinais clínicos e/ou lesões macroscópicas sugestivos de doença entérica. Infiltrados linfoistiocíticos de intensidades variáveis foram associados com marcação positiva anti-circovirus suíno tipo 2 (anti-PCV2) em amostras de intestinos e linfonodos mesentéri-cos de todos os 79 animais. Embora a maioria dos achados fossem semelhantes aos descritos em enterite associada com PCV2, marcação imuno-histoquímica anti-PCV2 foi associada com depleção de células caliciformes (24 suínos), atrofia e fusão de vilosidades do íleo (18 suínos) e dilatação de vasos linfáticos (11 suínos) combinada ou não com linfangite. Antígenos de PCV2 foram demonstrados por imuno-histoquímica no citoplasma e núcleo de células epiteliais intralesionais, histiócitos e células tipo endotelial Samples of mesenteric lymph nodes and intestines from 79 unthrifty 3-to 5-monthold postweaning pigs, confirmed as naturally affected with postweaning multisystemic wasting syndrome (PMWS), were studied. Pigs originated from 12 farms in southern Brazil and were selected on the basis of clinical signs and/or gross lesions suggestive of enteric disorder. Lymphohistiocytic infiltrates of varying intensity were associated with anti-porcine circovirus type 2 (anti-PCV2) immunostaining (IS) in samples of intestines and mesenteric lymph nodes from all pigs. Although most findings were similar to those described in PCV2-associated enteritis, anti-PCV2 IS in association with depletion of the goblet cell mucin stores (24 pigs), diffuse ileal villous atrophy and fusion (18 pigs), and dilatation of the lymphatic vessels (11 pigs) combined or not with lymphangitis were also observed. PCV2 antigen was immunohistochemically demonstrated in the cytoplasm and nuclei from intralesional epithelial cells, histiocytes, and endothelial-like cells in intestinal tissues. Together these findings imply an association with PCV2. The presence of co-infections by Lawsonia intracellularis, Brachyspira spp., Mycobacterium spp., Salmonella spp., rotavirus, parvovirus, coronavirus and enteric calicivirus with PCV2 in the intestinal lesions was investigated.

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“…Subsequent studies carried out on both healthy and symptomatic pigs at different ages have contributed to draw a more complete picture. Analysis of 102 faecal samples of nursing and weaning pigs with enteritis from 31 farms in Korea has revealed a low prevalence (8.8%) and all the samples were characterized as GGIII SaVs [41]. Analysis of 203 fecal samples collected from seven farms in a 10-years time span in Venezuela has revealed a high frequency of detection of GGIII SaVs (Cowden-like) in pigs between 3 and 4 weeks of age (39%), with almost equal frequencies of detection in healthy and diarrhoeic animals [42].…”

Section: Discussionmentioning

confidence: 99%

Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets

et al. 2008

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Enteric caliciviruses (noroviruses and sapoviruses) are responsible for the majority of non-bacterial gastroenteritis in humans of all age groups. Analysis of the polymerase and capsid genes has provided evidence for a huge genetic diversity, but the understanding of their ecology is limited. In this study, we investigated the presence of porcine enteric caliciviruses in the faeces of piglets with diarrhoea. A total of 209 samples from 118 herds were analyszd and calicivirus RNA was detected by RT-PCR in 68 sample (32.5%) and in 46 herds (38.9%), alone or in mixed infection with group A and C rotaviruses. Sequence and phylogenetic analysis of the calicivirus-positive samples characterized the majority as genogroup III (GGIII) sapoviruses. Unclassified caliciviruses, distantly related to the representatives of the other sapovirus genogroups, were identified in five herds, while one outbreak was associated with a porcine sapovirus related genetically to human GGII and GGIV sapovirus strains. By converse, norovirus strains were not detected. Altogether, these data suggest the epidemiological relevance of porcine enteric caliciviruses and suggest a role in the etiology of piglets diarrhoea.

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Detection and Molecular Characterization of Porcine Enteric Calicivirus in Korea, Genetically Related to Sapoviruses

Cited by 41 publications

References 13 publications

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Porcine enteric caliciviruses: Genetic and antigenic relatedness to human caliciviruses, diagnosis and epidemiology

Intestinal lesions in pigs affected with postweaning multisystemic wasting syndrome

Genetic heterogeneity of porcine enteric caliciviruses identified from diarrhoeic piglets

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