Detection and Localization of Wolbachia in Thrips palmi Karny (Thysanoptera: Thripidae)

Saurav, Gunjan Kumar; Daimei, Guisuibou; Rana, Vipin Singh; Popli, Sonam; Rajagopal, Raman

doi:10.1007/s12088-016-0567-7

Cited by 14 publications

(7 citation statements)

References 22 publications

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“…This bacterium can eliminate males, turn them into females, sterilize uninfected females or behave as a mutualistic symbiont 113 . Wolbachia has been detected both in arrhenotokous and thelytokous thrips species 108 , 109 , 111 although the frequency of this reproductive manipulators in Thysanoptera is unknown. The absence of Wolbachia , identified by conventional PCR, has been shown for three thrips: Frankliniella occidentalis 108 , Thrips tabaci 108 , 114 and Heliothrips haemorrhoidalis 115 .…”

Section: Discussionmentioning

confidence: 99%

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Kaczmarczyk

Kucharczyk

et al. 2018

Sci Rep

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Insects’ exoskeleton, gut, hemocoel, and cells are colonized by various microorganisms that often play important roles in their host life. Moreover, insects are frequently infected by vertically transmitted symbionts that can manipulate their reproduction. The aims of this study were the characterization of bacterial communities of four developmental stages of the fungivorous species Hoplothrips carpathicus (Thysanoptera: Phlaeothripidae), verification of the presence of Wolbachia, in silico prediction of metabolic potentials of the microorganisms, and sequencing its mitochondrial COI barcode. Taxonomy-based analysis indicated that the bacterial community of H. carpathicus contained 21 bacterial phyla. The most abundant phyla were Proteobacteria, Actinobacteria, Bacterioidetes and Firmicutes, and the most abundant classes were Alphaproteobacteria, Actinobacteria, Gammaproteobacteria and Betaproteobacteria, with different proportions in the total share. For pupa and imago (adult) the most abundant genus was Wolbachia, which comprised 69.95% and 56.11% of total bacterial population respectively. Moreover, similarity analysis of bacterial communities showed that changes in microbiome composition are congruent with the successive stages of H. carpathicus development. PICRUSt analysis predicted that each bacterial community should be rich in genes involved in membrane transport, amino acid metabolism, carbohydrate metabolism, replication and repair processes.

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Section: Discussionmentioning

confidence: 99%

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Kaczmarczyk

Kucharczyk

et al. 2018

Sci Rep

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“…tabaci [25,29]. Wolbachia has been reported in other thrips species such as Echinothrips americanus , Gynaikothrips ficorum , Suocerathrips linguis [29], Thrips palmi [63] and Hoplothrips carpathicus [42] but their role in T . tabaci is unclear.…”

Section: Discussionmentioning

confidence: 99%

Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)

et al. 2019

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The gut microbial community structure of adult Thrips tabaci collected from 10 different agro-climatically diverse locations of India was characterized by using the Illumina MiSeq platform to amplify the V3 region of the 16S rRNA gene of bacteria present in the sampled insects. Analyses were performed to study the bacterial communities associated with Thrips tabaci in India. The complete bacterial metagenome of T. tabaci was comprised of 1662 OTUs of which 62.25% belong to known and 37.7% of unidentified/unknown bacteria. These OTUs constituted 21 bacterial phyla of 276 identified genera. Phylum Proteobacteria was predominant, followed by Actinobacteria, Firmicutes, Bacteroidetes and Cyanobacteria. Additionally, the occurrence of the reproductive endosymbiont, Wolbachia was detected at two locations (0.56%) of the total known OTUs. There is high variation in diversity and species richness among the different locations. Alpha-diversity metrics indicated the higher gut bacterial diversity at Bangalore and lowest at Rahuri whereas higher bacterial species richness at T. tabaci samples from Imphal and lowest at Jhalawar. Beta diversity analyses comparing bacterial communities between the samples showed distinct differences in bacterial community composition of T. tabaci samples from different locations. This paper also constitutes the first record of detailed bacterial communities associated with T. tabaci. The location-wise variation in microbial metagenome profile of T. tabaci suggests that bacterial diversity might be governed by its population genetic structure, environment and habitat.

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“…Moreover, Pantoea ananatis and Pantoea agglomerans were detected in the Thrips tabaci gut by Dutta et al [20]. Other studies have demonstrated the presence of Wolbachia in Franklinothrips vespiformis [21], Scirtothrips perseae [22], Echinothrips americanus, Suocerathrips linguis, Gynaikothrips ficoru, Hercinothrips femoralis and Parthenothrips dracaenae [23], Thrips palmi [24], and Hoplothrips carpathicus [25]. Finding the genus Wolbachia in a pest species is important because it increases the potential for the pest's biological control.…”

Section: Introductionmentioning

confidence: 95%

Thrips Microbiome Study in Commercial Avocado (Persea americana Mill.) from Northwest Colombian Andes (Antioquia, Colombia) Shows the Presence of Wolbachia, Ehrlichia, Enterobacter

et al. 2022

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Microbiota associated with insects play several important roles in their host, including protection against pathogens, provision of nutrition, and survival in hostile environments. The aim of this work was to identify the bacterial community found in avocado thrips from Northwestern Colombia (Antioquia department) in order to find isolates for potential biocontrol purposes. Culture-dependent methods based on 16S rRNA and gyrase B gene sequencing in 42 bacterial isolates allowed the identification of the genera Bacillus, Serratia, Moraxella, Pantoea, and Sphingomonas. Microbial diversity detected with the temperature gradient gel electrophoresis (TGGE) technique on three morphotypes of thrips, named brown (Scirtothrips hansoni), black (Frankliniella panamensis), and pale (Frankliniella sp.), showed a low bacterial community density (Shannon–Wiener index = 1480, p > 0.05) with significant differences among morphotypes (R = 0.7877, p = 0.0004). Results obtained with Illumina sequencing on the V1–V2 hypervariable region of the subunit 16S rRNA showed a predominant sequence in the brown morphotype (Scirtothrips hansoni) that belongs to the genus Wolbachia. The 16S amplicon analyses were extended to more samples and higher resolution using the V4–V5 hypervariable region. The results showed six additional bacteria phyla, confirming the previous observation for the dominant bacterial groups made in S. hansoni and the detection of the alternation of highly predominant genera among these thrips. Our results demonstrate that endosymbiont such as Wolbachia sp. are part of the microbiota of these pests, thereby indicating the possibility of employing this type of bacterium to improve the management of avocado thrips globally.

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Detection and Localization of Wolbachia in Thrips palmi Karny (Thysanoptera: Thripidae)

Cited by 14 publications

References 22 publications

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

First insight into microbiome profile of fungivorous thrips Hoplothrips carpathicus (Insecta: Thysanoptera) at different developmental stages: molecular evidence of Wolbachia endosymbiosis

Microbiome profiling of the onion thrips, Thrips tabaci Lindeman (Thysanoptera: Thripidae)

Thrips Microbiome Study in Commercial Avocado (Persea americana Mill.) from Northwest Colombian Andes (Antioquia, Colombia) Shows the Presence of Wolbachia, Ehrlichia, Enterobacter

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