Detection and identification of hybrids in the coenopopulation of co-growing species of Cypripedium (Orchidaceae) in the Novosibirsk Region

Abstract

Cypripedium calceolus L. and C. macranthon Sw. (Orchidaceae) are rare species throughout almost the entire territory of Russia. Though the data on intraspecific genetic differentiation has been presented in the literature, the species of C. calceolus, C. macranthon and alleged hybrids of C. × ventricosum growing in sympatric population in the Iskitim District of the Novosibirsk Region (NR) have not been investigated yet. The purpose of this work is dentification of the rare species of Cypripedium calceolus, C. macranthon and detection of C. × ventricosum hybrids in the NR using morphometric parameters and ISSR markers. It was found the mature generative specimens of C. × ventricosum formed the largest number of shoots, compared with the parent species. According to the number of flowers on the generative shoot, the hybrid occupied an intermediate position. According to the ISSR analysis, components from both parental species were identified in each of the three hybrids studied. Therefore, the presence of C. × ventricosum hybrid plants in the local NR coenopopulation of co-growing species C. calceolus and C. macranthon was detected and confirmed based on the study of morphometric parameters and ISSR markers.