Detection and Genomic Characterization of Senecavirus A, Ohio, USA, 2015

Wang, Leyi; Prarat, Melanie; Hayes, Jeff; Zhang, Yan

doi:10.3201/eid2207.151897

Cited by 28 publications

(21 citation statements)

References 5 publications

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“…Foreign animal disease investigations indicated that other vesicular viral pathogens, such as foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), vesicular stomatitis virus (VSV), and vesicular exanthema of swine virus (VESV), were negative in these cases [10]. Subsequently, SVV detection was reported by other laboratories in the U.S. [11][12][13][14][15][16], China [17][18][19][20][21], Canada [22], Thailand [23], and Colombia [24]. Vesicular lesions were induced in pigs following experimental inoculation with the contemporary U.S. isolates of SVV [25,26], confirming that SVV is a vesicular viral pathogen.…”

Section: Introductionmentioning

confidence: 99%

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

et al. 2019

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Background Seneca Valley virus (SVV) has emerged in multiple countries in recent years. SVV infection can cause vesicular lesions clinically indistinguishable from those caused by other vesicular disease viruses, such as foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV), vesicular stomatitis virus (VSV), and vesicular exanthema of swine virus (VESV). Sensitive and specific RT-PCR assays for the SVV detection is necessary for differential diagnosis. Real-time RT-PCR (rRT-PCR) has been used for the detection of many RNA viruses. The insulated isothermal PCR (iiPCR) on a portable POCKIT™ device is user friendly for on-site pathogen detection. In the present study, SVV rRT-PCR and RT-iiPCR were developed and validated. Results Neither the SVV rRT-PCR nor the RT-iiPCR cross-reacted with any of the vesicular disease viruses (20 FMDV, two SVDV, six VSV, and two VESV strains), classical swine fever virus (four strains), and 15 other common swine viruses. Analytical sensitivities of the SVV rRT-PCR and RT-iiPCR were determined using serial dilutions of in vitro transcribed RNA as well as viral RNA extracted from a historical SVV isolate and a contemporary SVV isolate. Diagnostic performances were further evaluated using 125 swine samples by two approaches. First, nucleic acids were extracted from the 125 samples using the MagMAX™ kit and then tested by both RT-PCR methods. One sample was negative by the rRT-PCR but positive by the RT-iiPCR, resulting in a 99.20% agreement (124/125; 95% CI: 96.59–100%, κ = 0.98). Second, the 125 samples were tested by the taco™ mini extraction/RT-iiPCR and by the MagMAX™ extraction/rRT-PCR system in parallel. Two samples were positive by the MagMAX™/rRT-PCR system but negative by the taco™ mini/RT-iiPCR system, resulting in a 98.40% agreement (123/125; 95% CI: 95.39–100%, κ = 0.97). The two samples with discrepant results had relatively high C T values. Conclusions The SVV rRT-PCR and RT-iiPCR developed in this study are very sensitive and specific and have comparable diagnostic performances for SVV RNA detection. The SVV rRT-PCR can be adopted for SVV detection in laboratories. The SVV RT-iiPCR in a simple field-deployable system could serve as a tool to help diagnose vesicular diseases in swine at points of need.

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Section: Introductionmentioning

confidence: 99%

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

et al. 2019

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“…Furthermore, SVA was recently isolated from neonatal piglets and it is the first report linking SVA with severe neonatal death in America (Canning et al., ). Three reports described detection and genomic characterization of SVA in the United State in 2015 (Guo et al., ; Hause et al., ; Wang et al., ). The Brazilian study evaluated the SVA infection in neonatal piglets and suggested that urine might be a possible route for the dissemination of this virus (Leme et al., ).…”

Section: Discussionmentioning

confidence: 99%

The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China

Zhao

Bai

et al. 2016

Transbound Emerg Dis

138

113

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Senecavirus A (SVA) infection was recently confirmed in pigs in Brazil. In March, 2015, an outbreak of vesicular disease occurred in Guangdong, China, characterized by vesicular lesions in sows and acute death of neonatal piglets. Cumulative incidence of porcine idiopathic vesicular disease in farm A was 258, which had a total number of 5500 sows. Sows in farm B displayed typical vesicular symptoms by May, 2015, which also had 5500 sows. A total of 278 and 142 of 5500 sows in farm B demonstrated lame and presented vesicles, respectively, associated with a total of 186 mortality in piglets. Routine differential diagnoses for swine vesicular disease were carried out to exclude infection with foot-and-mouth disease virus, swine vesicular disease virus, vesicular exanthema of swine virus and vesicular stomatitis virus. In this study, seven pairs of primer were designed to amplify the complete genome of SVA in RT-PCR assays. Sequence alignment showed that this Chinese strain shares 94.4-97.1% sequence identity to other eight strains of SVA. This is the first report of SVA in China and provides information about the association between SVA infection and vesicular disease.

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“…Senecavirus A was first isolated in 2002 and the virus has also been discovered in North American and Brazil. Until recently, multiple strains were generated from Brazil ( n = 3), Canada ( n = 1), China ( n = 1) and the USA ( n = 20), bringing the total to 25 (Guo et al., ; Hales et al., ; Hause et al., ; Leme et al., , ; Vannucci et al., ; Wang et al., ; Wu et al., ). Research on the molecular epidemiology of SVA is limited, and cases are probably underreported.…”

Section: Discussionmentioning

confidence: 99%

“…Even though SVA pathogenesis in swine has not been described, SVA causes vesicular lesions in pigs that are clinically indistinguishable from other vesicular diseases. This includes clinical signs of acute lameness, sudden neonatal death and vesicular lesions previously reported in Brazil, United States and China (Singh, Corner, Clark, Scherba, & Fredrickson, ; Leme et al., ; Vannucci et al., ; Canning et al., ; Guo et al., ; Hause, Myers, Duff, & Hesse, ; Leme et al., ; Segales et al., ; Wang, Prarat, Hayes, & Zhang, ; Wu et al., ).…”

Section: Introductionmentioning

confidence: 94%

Phylogenetic and genome analysis of seven senecavirus A isolates in China

Zhao

Bai

et al. 2017

Transbound Emerg Dis

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Senecavirus A (SVA) is the only member of genus Senecavirus that causes vesicular lesions in pigs. We have characterized seven SVA isolates from different swine farms in Guangdong, China. The most variable isolate, CH-DL-01-2016, contained a single amino acid insertion at position 219-220 and a 16 amino acid insertion at position 250-251. The VP1 protein also had four nucleotide changes when compared to 31 other SVA VP1 sequences obtained from GenBank. These mutations have not been identified before. Phylogenetic trees demonstrated that the new SVA isolates were clustered into two different clades and shared 96.3%-97.1% similarity with US strains and 97.9%-98.3% similarity with Brazilian stains on nucleotide level, respectively. Prediction of antigenic epitope indicated that SVA VP1 protein contained both potential B-cell and potential T-cell epitopes. This report provides information about variation analysis in SVA in southern China.

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Detection and Genomic Characterization of Senecavirus A, Ohio, USA, 2015

Cited by 28 publications

References 5 publications

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

Development and evaluation of a real-time RT-PCR and a field-deployable RT-insulated isothermal PCR for the detection of Seneca Valley virus

The First Identification and Complete Genome of Senecavirus A Affecting Pig with Idiopathic Vesicular Disease in China

Phylogenetic and genome analysis of seven senecavirus A isolates in China

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