Detection and determination of organophosphorus insecticides in tissues by thin-layer chromatography

Biochemical and Toxicological Investigations Related to OP Compounds

“…Thin layer chromatography (TLC) OP compounds are quite amenable to detection and identification by this relatively simple and inexpensive technique and a considerable number of methods have been published (Antoine and Mees, 1971;Tewari and Harpalani, 1977;Bakry and AbouDonia, 1980;Kurhekar, Pundik, Meghal, 1980;Ziminski and Manning, 1981;Erdman, Brose, Schultz, 1990;Sevalkar, Patil, Katkar, 1991;Futagami et al, 1997 The method proposed by Antoine and Mees (1971) was designed to detect and identify upwards of 25 compounds and two types of adsorbent for the chromatography (silica gel and polyamide), seven developing solvent systems and seven visualisation techniques were examined. As a result of this thorough investigation, they were able to recommend a procedure that used a maximum of four consecutive chromatograms with the OP compounds detected by UV absorption and spraying the plates with either nitropyridine or palladium chloride.…”

“…As a result of this thorough investigation, they were able to recommend a procedure that used a maximum of four consecutive chromatograms with the OP compounds detected by UV absorption and spraying the plates with either nitropyridine or palladium chloride. Tewari and Harpalani (1977) were concerned with tissue analysis and developed a complicated technique of extracting macerated samples of liver, kidney, intestine, spleen, heart, lung and brain by refluxing with hexane and then re-extracting the compounds into acetonitrile. This was then diluted 10 times with water, saturated with sodium sulphate and re-extracted with hexane.…”

Cypermethrin insecticide in viscera of suspected snake bite case: A Forensic case study

“…Chromatographic techniques are discussed in literature analyzed for poisons in food 2 , food e of 11 pesticide residue samples from 70 postmortem cases is discussed 3 by Shailesh Kumar Rai etal. Thin layer chromatography is commonly used in organophosphorus pesticide analysis and tremendous work is done by using this technique [4][5][6] . The review of Mass spectrometry in application of toxicology revealed that the technique is really useful in forensic toxicology 7 It is possible to analyse inorganic poisons as well by using techniques like ICP-MS, whereas LC-MS and GC-MS are used for analysis of organic poisons [7][8] .…”