Detection and characterization of virulence factors in lactose positive and lactose negative Salmonella serovars isolated from seafood

Kumar, Rajesh; Surendran, P.K.; Thampuran, Nirmala

doi:10.1016/j.foodcont.2008.06.005

Cited by 19 publications

(12 citation statements)

References 32 publications

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“…and serovars Enteritidis and Typhimurium. In recent years, many studies have been published using specific primers complementary to virulence genes in combination with different pre-enrichment strategies (Cardona-Castroa et al , 2009, Kumar at al., 2009, Lim et al , 2003). Soumet et al (1999) developed an m-PCR assay, but they were not able to detect Salmonella without the isolation of suspicious colonies.…”

Section: Resultsmentioning

confidence: 99%

Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay

Paião¹,

Arisitides²,

Murate³

et al. 2013

Braz. J. Microbiol.

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The presence of Salmonella in the intestinal tract, on the chickens skin and among their feathers, may cause carcasses contamination during slaughtering and processing and possibly it is responsible by the introduction of this microorganism in the slaughterhouses. A rapid method to identify and monitor Salmonella and their sorovars in farm is becoming necessary. A pre-enriched multiplex polymerase chain reaction (m-PCR) assay employing specific primers was developed and used to detect Salmonella at the genus level and to identify the Salmonella enterica serovar Enteritidis (S. Enteritidis) and Salmonella enterica serovar Typhimurium (S. Typhimurium) in broiler chicken swab samples. The method was validated by testing DNA extract from 90 fresh culture cloacal swab samples from poultry chicken cultured in phosphate buffer peptone water at 37 °C for 18 h. The final results showed the presence of Salmonella spp. in 25% of samples, S. Enteritidis was present in 12% of the Salmonella-positive samples and S. Typhimurium in 3% of the samples. The m-PCR assay developed in this study is a specific and rapid alternative method for the identification of Salmonella spp. and allowed the observation of specific serovar contamination in the field conditions within the locations where these chickens are typically raised.

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Section: Resultsmentioning

confidence: 99%

Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay

Paião¹,

Arisitides²,

Murate³

et al. 2013

Braz. J. Microbiol.

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“…More isolates from different parts of the world and from different origins need to be tested to confirm that the ST543 sequence type was a major worldwide clone based on MLST analysis. Several PCR-based procedures for the detection of known DNA sequences, including virulence genes, have been successfully applied for many Salmonella serovars [15,25,[28][29][45][46]. The main advantages of these approaches were the simplicity, rapidity, and cost effectiveness, which means that many strains could be easily virulotyped [15,32] .…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial and molecular analysis of Salmonella serovar Livingstone strains isolated from humans in Tunisia and Belgium

Guedda

Taminiau

Ferjani

et al. 2014

J Infect Dev Ctries

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Introduction: Salmonella Livingstone is one of the most common serotypes responsible for nosocomial outbreaks in Tunisia. In this study, 42 isolates of Salmonella Livingstone were analyzed. Most of these were isolated from humans (31 strains from Tunisia and 9 strains from Belgium) and 2 isolates came from food products (beef and pork). Methodology: All strains were characterized by antibiogram, multilocus sequence typing (MLST), and virulotyping. This last technique was carried out by simple PCR of five chromosomal genes (agfA, hin/H2, iroB, phoP/Q, and slyA) and two plasmid genes (spvA and spvC). Results: All Tunisian strains were resistant to amoxicillin, amoxicillin-clavulanic acid, ticarcillin, cefalotin, gentamicin, and kanamycin. They were also resistant to third-generation cephalosporin antibiotics (cefotaxim and ceftazidim). Belgian isolates were susceptible to all antibiotics tested. Further to MLST analyses, Tunisian strains belonged to the same sequence type, ST543. For Belgian isolates, eight strains had a ST543 profile, two strains had a ST638 profile, and one strain had a ST457 profile. Analyses of the virulence gene contents showed that strains isolated in different years and from different origins had the same virulence profile. These carried all five chromosomal genes and lacked plasmid-located virulence genes spvA and spvC. Conclusions: A combination of different typing methods showed that the majority of Belgian strains and all Tunisian strains were closely related; they belonged to the same sequence type (ST543) and had the same virulence profile, but different antibiotic resistance profiles depended on the country of origin.

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“…Up to 10-15% of fish samples from India and Mexico were positive of Salmonella which has also been detected in several crustacean and molluscan products from India and Malaysia (Huss & Gram 2003). Salmonella contamination in fish and fishery products has also been reported from other countries like Thailand, Hong Kong, Spain and Turkey (Herrera et al, 2006;Kumar et al, 2009;Pamuk et al, 2011). The highest Salmonella incidence in fishery products was determined in Central Pacific and African countries while it was lower in Europe and including Russia, and North America (Heinitz et al 2000).…”

Section: Occurrence In Fish and Fishery Productmentioning

confidence: 82%

Salmonella in Fish and Fishery Products

Olgunoğlu¹

2012

Salmonella - A Dangerous Foodborne Pathogen

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Detection and characterization of virulence factors in lactose positive and lactose negative Salmonella serovars isolated from seafood

Cited by 19 publications

References 32 publications

Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay

Detection of Salmonella spp, Salmonella Enteritidis and Typhimurium in naturally infected broiler chickens by a multiplex PCR-based assay

Antimicrobial and molecular analysis of Salmonella serovar Livingstone strains isolated from humans in Tunisia and Belgium

Salmonella in Fish and Fishery Products

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