Detection and characterization of type B influenza virus from influenza-like illness cases during the 2017–2018 winter influenza season in Beijing, China

Zhu, Dong; Lok, Chonghou; Chao, S; Li, Runqing; Zhao, Zhen; Dong, Jianfei; Qin, Kun; Zhao, Xiuying

doi:10.1007/s00705-019-04160-w

Cited by 15 publications

(14 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In total, influenza B virus accounted for the maximum number of laboratory-confirmed influenza cases, followed by pandemic A/H1N1 and seasonal A/H3N2. These results corresponded with the facts that a co-epidemic pattern of these three strains of influenza virus has formed in China since 2009, with seasonal A/H3N2 predominantly circulating in the south of China while influenza B virus and pandemic A/ H1N1 are more frequent in northern China [4,18,19,[33][34][35]. It is noteworthy that the number of influenza-positive cases was much lower in 2011 and 2012 than other years.…”

supporting

confidence: 82%

Epidemiological features and time-series analysis of influenza incidence in urban and rural areas of Shenyang, China, 2010–2018

et al. 2020

View full text Add to dashboard Cite

In recent years, there have been a significant influenza activity and emerging influenza strains in China, resulting in an increasing number of influenza virus infections and leading to public health concerns. The aims of this study were to identify the epidemiological and aetiological characteristics of influenza and establish seasonal autoregressive integrated moving average (SARIMA) models for forecasting the percentage of visits for influenza-like illness (ILI%) in urban and rural areas of Shenyang. Influenza surveillance data were obtained for ILI cases and influenza virus positivity from 18 sentinel hospitals. The SARIMA models were constructed to predict ILI% for January–December 2019. During 2010–2018, the influenza activity was higher in urban than in rural areas. The age distribution of ILI cases showed the highest rate in young children aged 0–4 years. Seasonal A/H3N2, influenza B virus and pandemic A/H1N1 continuously co-circulated in winter and spring seasons. In addition, the SARIMA (0, 1, 0) (0, 1, 2)12 model for the urban area and the SARIMA (1, 1, 1) (1, 1, 0)12 model for the rural area were appropriate for predicting influenza incidence. Our findings suggested that there were regional and seasonal distinctions of ILI activity in Shenyang. A co-epidemic pattern of influenza strains was evident in terms of seasonal influenza activity. Young children were more susceptible to influenza virus infection than adults. These results provide a reference for future influenza prevention and control strategies in the study area.

show abstract

supporting

confidence: 82%

Epidemiological features and time-series analysis of influenza incidence in urban and rural areas of Shenyang, China, 2010–2018

et al. 2020

View full text Add to dashboard Cite

show abstract

“…There was an epidemic of the B/Yamagata strain in winter of 2017 in Beijing [12,13], the epidemic was also confirmed by our group using RT-PCR and by data from China CDC [13,18]. By analyzing unpaired and paired data of antigen to IgM Abs, we found that antigen to Flu B was significantly increased in 2017 winter compared with that in 2016, while there was no simultaneous increase in IgM Abs to Flu B.…”

Section: Discussionmentioning

confidence: 60%

“…By analyzing unpaired and paired data of antigen to IgM Abs, we found that antigen to Flu B was significantly increased in 2017 winter compared with that in 2016, while there was no simultaneous increase in IgM Abs to Flu B. Since sensitivity of antigen detection by IRDT is lower than by RT-PCR [18,34], the actual Flu B infection should be severer than that indicated by antigen-positivity rate. Thus, some IgM Abs to Flu B in the 2017 winter flu season should have been present, but not detected by the single assay.…”

Section: Discussionmentioning

confidence: 99%

“…The reagent is coated with anti-influenza A and anti-influenza B NP in one strip, enabling differentiation between Flu A and Flu B. Undiluted swabs were used for the detection, and all specimens were tested in a single experiment. The IRDT method had been compared with RT-PCR methods, the positive consistency was 54.3% and negative consistency was 97.3% and the two methods had concordance at 86.9% for Flu B; the positive consistency was 47.83% and negative consistency was 98.65% and the two methods had concordance at 87.82% for Flu A [18].…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Unreliable usage of a single influenza virus IgM antibody assay in influenza-like illness: A retrospective study of the 2016–2018 flu epidemic

Yao¹,

Zhao²,

Song³

et al. 2019

PLoS ONE

View full text Add to dashboard Cite

We retrospectively analyzed serum IgM antibodies (Abs) to influenza viruses from two tertiary hospitals in Beijing from December 2016 to February 2018. Samples from 36,792 patients, aged 0–98 years, were collected and tested. Among the patients, 923 children from two winter flu seasons were assayed with both antigens and IgM Abs to Flu A and Flu B and assigned as paired groups. Another 2,340 adults and 1,978 children with only antigen tested in the 2016 and 2017 winter flu seasons were named as unpaired groups. IgM Abs-positivity rates in children were 0.80% and 36.57% for Flu A and Flu B, respectively, peaking at 4–5 years of age. For adults, the Flu A and Flu B IgM Abs-positivity rates were 10.34% and 21.49%, respectively, peaking at 18–35 years of age. The trend of temporal distribution between the children and the adults was significantly correlated for IgM Abs to Flu B, but not for Flu A. Compared with unpaired groups, the detection rate of Flu A antigen was significantly higher than IgM Abs in children, whereas frequencies of IgM Abs were higher than antigen in adults. Incidence of Flu B antigen was sharply increased in 2017 winter than in the 2016 winter in both children and adults, but no concomitant increase was observed in IgM Abs to Flu B. For paired children groups, incidence of Flu B antigen in the 2017 flu season was significantly higher than that in the 2016 flu season; in contrast, positive rates of IgM Abs in the 2017 flu season were even lower than those in 2016. Considering antigen detection may reflect the Flu A/Flu B epidemic, our results indicate single-assayed IgM Abs were less effective in the diagnosis of acute influenza virus infection, and the use of this assay for epidemiology evaluations was not supported by these findings.

show abstract

“…Pathogen diagnosis of influenza-like illness (ILI) has received increasing attention due to outbreaks of influenza and increased threat to human health from avian influenza (Bedford et al 2015; Li et al 2014; Zhu et al 2019). Prompt and accurate detection of influenza virus is essential for patients with ILI, which helps to avoid unnecessary use of antibiotic therapy and to prevent nosocomial transmission of influenza virus (Neuzil et al 2000; Townsend and Eiland 2006).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of a novel micro/nanofluidic chip platform for the detection of influenza A and B virus in patients with influenza-like illness

Gai²,

Zhu

et al. 2019

AMB Expr

Self Cite

View full text Add to dashboard Cite

We introduced a novel micro/nanofluidic chip platform (MNCP), which is based on an isothermal nucleic acid amplification method. This study aimed to evaluate the MNCP method for influenza A and B viruses detecting and subtyping using throat swab samples from patients with influenza-like illness (ILI). A total of 266 throat swab samples from 266 non-repeated patients with ILI were tested for influenza A and B viruses using three methods, MNCP, a rapid influenza diagnostic test (RIDT), and real-time reverse transcription polymerase chain reaction (rRT-PCR). The results of MNCP were compared to those obtained by rRT-PCR and RIDT and the performance of MNCP was further evaluated. Compared with rRT-PCR results, the rates of sensitivity, specificity, overall concordance, and the kappa value of MNCP were 98.89%, 96.97%, 97.65%, and 0.95 for influenza A virus; 94.95%, 99.38%, 97.68%, and 0.95 for influenza B virus, respectively. Subtypes of influenza A viruses, e.g., A(H1N1)pdm09, A(H3N2), and A(not subtyped), and influenza B viruses could be distinguished in one MNCP assay within 1 h. Compared with rRT-PCR and MNCP, RIDT showed poor clinical sensitivity for influenza virus detection. This study showed MNCP is rapid, sensitive and versatile detecting system with potential for clinical application in pathogen diagnosis for patients with ILI.

show abstract

Detection and characterization of type B influenza virus from influenza-like illness cases during the 2017–2018 winter influenza season in Beijing, China

Cited by 15 publications

References 38 publications

Epidemiological features and time-series analysis of influenza incidence in urban and rural areas of Shenyang, China, 2010–2018

Epidemiological features and time-series analysis of influenza incidence in urban and rural areas of Shenyang, China, 2010–2018

Unreliable usage of a single influenza virus IgM antibody assay in influenza-like illness: A retrospective study of the 2016–2018 flu epidemic

Evaluation of a novel micro/nanofluidic chip platform for the detection of influenza A and B virus in patients with influenza-like illness

Contact Info

Product

Resources

About