Detection and Characterization of Rotavirus G and P Types from Children Participating in a Rotavirus Vaccine Trial in Belém, Brazil

Mascarenhas, Joana D’Arc Pereira; Linhares, Alexandre da Costa; Gabbay, Yvone Benchimol; Leite, José Paulo Gagliardi

doi:10.1590/s0074-02762002000100020

Cited by 36 publications

(31 citation statements)

References 35 publications

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“…Also, the VP7, NSP1, NSP4, and VP6-encoding gene segments of the neonatal G9P [11] strain 116E have been shown to be of human origin, whereas VP4 was of bovine origin (28,29,40). Other successful animal-like HRVs, with a porcine-like VP7 of G5 serotype specificity, have been detected in Brazil, Argentina, and Paraguay since the early 1980s (10,17,26,43,70) that were shown to be, by either RNA-RNA hybridization or sequence analysis, naturally occurring reassortants between members of the human Wa and porcine rotavirus genogroups (3). Finally, the nearly complete sequence analysis of the unusual G9P [19] HRV strains RMC321, with long electropherotype and SGI specificity, detected during an epidemic of infantile gastroenteritis in Manipur, India, in 1987 and 1998, revealed that at least seven genome segments were clearly of porcine origin (62,96).…”

Section: Discussionmentioning

confidence: 99%

Full Genomic Analysis of Human Rotavirus Strain B4106 and Lapine Rotavirus Strain 30/96 Provides Evidence for Interspecies Transmission

Matthijnssens

Rahman

Martella

et al. 2006

J Virol

205

167

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The Belgian rotavirus strain B4106, isolated from a child with gastroenteritis, was previously found to have VP7 (G3), VP4 (P[14]), and NSP4 (A genotype) genes closely related to those of lapine rotaviruses, suggesting a possible lapine origin or natural reassortment of strain B4106. To investigate the origin of this unusual strain, the gene sequences encoding VP1, VP2, VP3, VP6, NSP1, NSP2, NSP3, and NSP5/6 were also determined. To allow comparison to a lapine strain, the 11 double-stranded RNA segments of a European G3P[14] rabbit rotavirus strain 30/96 were also determined. The complete genome similarity between strains B4106 and 30/96 was 93.4% at the nucleotide level and 96.9% at the amino acid level. All 11 genome segments of strain B4106 were closely related to those of lapine rotaviruses and clustered with the lapine strains in phylogenetic analyses. In addition, sequence analyses of the NSP5 gene of strain B4106 revealed that the altered electrophoretic mobility of NSP5, resulting in a super-short pattern, was due to a gene rearrangement (head-to-tail partial duplication, combined with two short insertions and a deletion). Altogether, these findings confirm that a rotavirus strain with an entirely lapine genome complement was able to infect and cause severe disease in a human child.Group A rotaviruses (family Reoviridae) are important enteropathogens of humans and of a large variety of mammals and birds (11, 37). The rotavirus genome consists of 11 segments of double-stranded RNA (dsRNA) encoding six structural viral proteins (VP) and six nonstructural proteins (NSP) (37). A mature infectious rotavirus particle is composed of three concentric layers, consisting of a protein core, an inner protein capsid, and an outer protein capsid (84). The outer protein layer consists of VP4 and VP7, the two independent neutralization antigens of the virus, defining 26 P (proteasesensitive) and 15 G (glycoprotein) types, respectively (37,55,68,69,85). The G and P types are peculiarly distributed across the various animal species (88), suggesting host species barriers and restriction, although a number of unusual G and P types, regarded as animal-like strains, have been identified in humans in different parts of the world (85, 88).Although rotaviruses infect particular species preferentially for which they have been defined as the homologous strains, heterologous rotavirus infections occur in both natural and experimental circumstances. Studies in the rabbit and mouse models have demonstrated that only homologous virus strains replicate efficiently and spread horizontally (21,38,44). Based on a Jennerian approach, animal strains that are naturally attenuated in humans have been exploited for the construction of candidate rotavirus vaccines for humans. In a number of field trials with such candidate rotavirus vaccines, the rhesus rotavirus (RRV) strain MMU18006 and the bovine strains NCDV, UK, and WC3 were shown to replicate to a lower extent in humans than in their homologous hosts but to induce immune responses (24, 2...

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Section: Discussionmentioning

confidence: 99%

Full Genomic Analysis of Human Rotavirus Strain B4106 and Lapine Rotavirus Strain 30/96 Provides Evidence for Interspecies Transmission

Matthijnssens

Rahman

Martella

et al. 2006

J Virol

205

167

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“…In this study we did not test for the possible presence of serotype G5 in fecal samples, because up to now this type has been rare among humans, having been found only in Brazilian (23,31) and Argentinean (7) children. In Spain, G5 rotavirus strains have not been detected in human clinical samples.…”

Section: Discussionmentioning

confidence: 99%

Diversity of Group A Human Rotavirus Types Circulating over a 4-Year Period in Madrid, Spain

Sánchez-Fauquier

Wilhelmi²,

Colomina

et al. 2004

J Clin Microbiol

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The incidence and distribution of human rotavirus G types among children under 5 years old with acute gastroenteritis were determined over a 4-year period (1998 to 2002) by using monoclonal antibodies and reverse transcription-PCR methods. Rotavirus was detected in 1,155 (31%) of 3,760 specimens tested. Rotavirus was studied in every month of the 48-month survey period. Rotavirus activity occurred mainly (51%) in the typically cooler months in Spain (November to February). The age distribution of rotavirus-positive cases showed that 90% of patients (1,038 of 1,155) were under 2 years old. Rotavirus types were determined for 576 of 1,155 patients (50%). G1 was the main genotype detected (53%), and the second most common was G4 (24%). The G2, G9, and G3 rotavirus types were detected in 14, 6, and 2% of the cases, respectively. Dual infections were detected in only 0.6%. The seasonal distribution of genotypes showed a significant genotypic shift: whereas G4 strains predominated (57%) during the 1998 to 2000 seasons, the G1 gradually increased to account for 75% in the 2000 to 2002 seasons. In addition, the present study reports the first detection of the G9 genotype in human fecal samples in Spain. Therefore, additional types may be required for vaccine development strategies that currently target only types G1 to G4.

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“…Rotavirus strain surveillance studies have been conducted annually since 1982 in various locations in Brazil (1,2,24,28,34,36,39,44). However, a systematic rotavirus surveillance study has never been conducted in the city of Salvador, located in the state of Bahia in Northeast Brazil.…”

mentioning

confidence: 99%

Predominance of Rotavirus Genotype G9 during the 1999, 2000, and 2002 Seasons among Hospitalized Children in the City of Salvador, Bahia, Brazil: Implications for Future Vaccine Strategies

et al. 2005

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Detection and Characterization of Rotavirus G and P Types from Children Participating in a Rotavirus Vaccine Trial in Belém, Brazil

Cited by 36 publications

References 35 publications

Full Genomic Analysis of Human Rotavirus Strain B4106 and Lapine Rotavirus Strain 30/96 Provides Evidence for Interspecies Transmission

Full Genomic Analysis of Human Rotavirus Strain B4106 and Lapine Rotavirus Strain 30/96 Provides Evidence for Interspecies Transmission

Diversity of Group A Human Rotavirus Types Circulating over a 4-Year Period in Madrid, Spain

Predominance of Rotavirus Genotype G9 during the 1999, 2000, and 2002 Seasons among Hospitalized Children in the City of Salvador, Bahia, Brazil: Implications for Future Vaccine Strategies

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