Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231

Lauková, Andrea; Mareková, Mária; Javorský, P.

doi:10.1111/j.1472-765x.1993.tb01413.x

Cited by 107 publications

(70 citation statements)

References 15 publications

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“…The identified enterococci represent an obligatory species also detected in other poultry (Lauková and Mich-lovičová, 2009), but they have been detected in other animals as well, such as rabbits or dogs Lauková 2004, Marciň áková et al 2006). In general, the most detected species in animal faeces is E. faecium (Lauková et al 1993, Lauková et al 1996, Devriese et al 1995, Strompfová and Lauková 2007, Lauková et al 2008. The phenotypic identification was in accordance with those claimed for type strains for the species reported in de Vos et al (2009).…”

Section: Discussionsupporting

confidence: 75%

“…Semi-purified substances of Ents represent small, thermo-stable peptides. They were prepared as previously described by Lauková et al (2012b) for EntEM41 (produced by E. faecium EM41 from ostrich-Emu); Strompfová and Lauková (2007) for Ent55 (produced by chicken isolate E. faecium EF55); Lauková et al (1993) for Ent4231 (produced by ruminal E. faecium CCM4231); Mareková et al (2003Mareková et al ( , 2007 for Ents EK13=(A, P) and EntM produced by environmental isolates E. faecium EK13=CCM7419 and AL41; Simonová and Lauková (2007) for Ent2019 produced by rabbit isolate E.faecium 2019-CCM7420. Inhibitory activity was defined as the reciprocal of the highest dilution producing an inhibitory zone against the indicator strain and expressed in Arbitrary Units per ml (AU/mL) against the principal indicator strain E. avium EA5 (from our laboratory).…”

Section: Susceptibility Of Enterococcus Faecalis Strains To Enterocinsmentioning

confidence: 99%

“…E. faecium CCM 4231 (Lauková et al 1993) was the control strain. The strains were cultivated in Brian Heart Infusion (Oxoid) overnight at 37 o C. 100 μL was plated onto MRS agar enriched with defibrinated sheep blood (Becton and Dickinson) and disks were applied.…”

Section: Antibiotic Susceptibility Testingmentioning

confidence: 99%

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Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Kandričáková¹,

Lauková²,

Strompfová³

2015

Polish Journal of Veterinary Sciences

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With an increasing number of pheasants as gamebirds being reared each year, these species are becoming a more prominent part of the workload of many veterinary practices. Only limited information can be found concerning the microflora of common pheasants. A significant part of the obligate microflora consists of lactic acid bacteria, including enterococci. In this study, faeces were sampled from 60 pheasants aged 16-17 weeks. Enterococcal counts reached 5.48±1.9 (log10) CFU/g. Strains (17) were taxonomically classified to the genus Enterococcus using the Maldi-Tof identification system; they were allotted to the species E. hirae (58.8%), E. faecium (23.5%) and E. faecalis (17.7%) by highly probable species identification or by secure genus identification/probable species identication. Species allocation was also confirmed using conventional biochemical tests. Most strains formed β-hemolysis. Gelatinase active phenotype was found in three E. faecalis strains. Enterococci were β-glucuronidase negative, mostly trypsin negative with slight or moderate production of α-chymotrypsin. EH52b and EF42 strains possessed the highest potential for pathogenicity. Average value of lactic acid was 1.78±0.33 mmo/L. Most strains were tetracycline resistant (82.4%). Polyresistant E. faecalis strains with positive gelatinase phenotype and possessing virulence factor genes confirmed using PCR (gelE, efaAfs, ccf cob, cpd) were sensitive to enterocins (activity 1600-25 600 AU/mL).

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Section: Discussionsupporting

confidence: 75%

Section: Susceptibility Of Enterococcus Faecalis Strains To Enterocinsmentioning

confidence: 99%

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Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Kandričáková¹,

Lauková²,

Strompfová³

2015

Polish Journal of Veterinary Sciences

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“…Numbers of viable cells were estimated at 0 h and after 24 h of incubation using M-Enterococcus agar. Enterococcus faecium CCM 4231 was used as a positive control (Lauková et al, 1993).…”

Section: Methodsmentioning

confidence: 99%

Enterococci from rabbits - potential feed additive

Simonová¹,

Lauková²,

Štyriak³

2005

CZECH J ANIM SCI

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Enterococci (58) from faeces of rabbits of various age (from 2 months to 3 years) and 5 different rabbit farms were isolated and tested for survival in the presence of oxgall, lactic acid production, urease activity, resistance to low pH as well as their binding ability was tested. Fifty percent of enterococcal isolates were identified as Enterococcus faecium, 19% as E. faecalis. All strains showed good survival in the presence of 5% oxgall. The urease activity of isolates was in the range from 0.013 to 17.13 nkat/ml, only E. faecalis EE229 strain did not produce any urease. The survival of strains was tested at pH 3.0 and the percentage of their survival ranged between 62.0% and 90.0%. E. faecium EF1819 strain was found to show the best survival ability at low pH. Particle agglutination assay values of selected enterococci expressed only negative (0) or weakly positive (1) binding of heparin, bovine fibrinogen, porcine fibronectin and lactoferrin. Based on the results, most of the selected enterococci could be promising probiotic feed additives.

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“…The most frequent bacteriocinproducing strains were detected among lactic acid bacteria, including enterococci . Enterocins, bacteriocins produced by enterococci have been studied in more detail during the last decade (Laukova et al, 1993;Aymerich et al, 1996;Casaus et al, 1997;Floriano et al, 1998;Marekova et al, 2000). Enterococcus faecium EK13 is a strain which produces enterocin A (Ent A) (Marekova et al, 2003).…”

Section: Inhibition Of Salmonella Enterica Serovar Dusseldorf By Entementioning

confidence: 99%

Inhibition of Salmonella enterica serovar Dusseldorf by enterocin A in gnotobiotic Japanese quails

Lauková¹,

Guba²,

Nemcová³

et al. 2004

Vet. Med. - Czech

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ABSTRACT:The protective (ENT1) and therapeutic (ENT2) effects of enterocin A (Ent), produced by Enterococcus faecium EK13, against Salmonella enterica serovar Dusseldorf SA31 was determined in a model of gnotobiotic Japanese quails. Twenty-one 3 days old birds were divided into 3 groups of equal size; (ENT1, ENT2 and control group -CG). They were experimentally infected with SA31 (10 7 cfu/ml) per os. For the group ENT1, Ent A (200 µl of 25 600 AU/ml) was administered 8 h before infection with SA31 strain and for the group ENT2, treatment with Ent A was administered 8 h a�er infection; CG was infected with SA31 and not treated with Ent A. Sampling of the feces was performed 8, 24, 48 and 168 h a�er infection. At the end of the experiment also the content of the caecum and ileum was analyzed. A log 1.37 reduction of SA31 colonization in feces of the group ENT1 was found a�er 8 h in comparison with CG. A�er 24 h, a significant difference in SA31 colonization was observed when comparing CG and ENT2. A�er 48 h, a lower colonization of SA31 was found in both groups which continued until the end of the experiment (168 h). At the same time, reduction of Salmonella enterica serovar Dusseldorf was detected in the content of the caecum (2.44 log) and ileum (3.16 log) in ENT2 but not in ENT 1 when compared with control group. These observations indicate stronger therapeutic effect of Ent A than prophylactic one in the digestive tract of gnotobiotic Japanese quails.

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Detection and antimicrobial spectrum of a bacteriocin-like substance produced by Enterococcus faecium CCM4231

Cited by 107 publications

References 15 publications

Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Characteristic and susceptibility to enterocins of enterococci in pheasants possessing virulence factor genes

Enterococci from rabbits - potential feed additive

Inhibition of Salmonella enterica serovar Dusseldorf by enterocin A in gnotobiotic Japanese quails

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