Detection and analysis of the microdistribution of uranium in the gills of freshwater Corbicula fluminea by SIMS technique

Tessier, Christine; Suhard, David; Simon, Olivier; Floriani, Magali; Rebière, François; Jourdain, Jean

doi:10.1016/j.nimb.2009.03.096

Cited by 3 publications

(4 citation statements)

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“…But a few years ago, the analysis of biological samples by ion microscopy was developed in the biomedical, nuclear, and botanic fields~Clerc et al, 1997;Grignon et al, 1997;Hidié et al, 2001;Kahn et al, 2002;Guerquin-Kern et al, 2005;Tessier et al, 2009!. As part of this research program, it was interesting to visualize the uranium accumulation in the different structures of renal cortex. by secondary ion mass spectrometry~SIMS!…”

Section: Introductionmentioning

confidence: 99%

“…The SIMS technique, introduced in the early 1960s by Castaing and Slodzian (1981), is used for the chemical characterization of solid surfaces mainly in geochemistry, microelectronics, and material sciences. But a few years ago, the analysis of biological samples by ion microscopy was developed in the biomedical, nuclear, and botanic fields (Clerc et al, 1997; Grignon et al, 1997; Hidié et al, 2001; Kahn et al, 2002; Guerquin-Kern et al, 2005; Tessier et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…The SIMS technique, introduced in the early 1960s by Castaing and Slodzian~1981!, is used for the chemical characterization of solid surfaces mainly in geochemistry, microelectronics, and material sciences. But a few years ago, the analysis of biological samples by ion microscopy was developed in the biomedical, nuclear, and botanic fields~Clerc et al, 1997;Grignon et al, 1997;Hidié et al, 2001;Kahn et al, 2002;Guerquin-Kern et al, 2005;Tessier et al, 2009!. As part of this research program, it was interesting to visualize the uranium accumulation in the different structures of renal cortex. In previous studies the uranium concentrations have been measured in the whole organ, but the distribution of this element in the different segments of nephron is not known as well as the sites selectivity of uranium retention within each structure.…”

Section: Introductionmentioning

confidence: 99%

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Uranium Microdistribution in Renal Cortex of Rats after Chronic Exposure: A Study by Secondary Ion Mass Spectrometry Microscopy

et al. 2012

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For a few years, the biological effects on ecosystems and the public of the bioaccumulation of radionuclides in situations of chronic exposures have been studied. This work, in keeping with the ENVIRHOM French research program, presents the uranium microdistribution by secondary ion mass spectrometry (SIMS) technique in the renal cortex of rats following chronic exposure to this low level element in the drinking water (40 mg/L) as a function to exposure duration (6, 9, 12, and 18 months). The SIMS mass spectra and 238U+ ion images produced with a SIMS CAMECA 4F-E7 show the kinetic of uranium accumulation in the different structures of the kidney. For the rats contaminated up to 12 months, the radioelement is mainly fixed in the proximal tubules; then after 18 exposure months, uranium is detected in all the segments of the nephron. This work has also shown that ion microscopy is an analytical method to detect trace elements and give elemental cartography at the micrometer scale.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Uranium Microdistribution in Renal Cortex of Rats after Chronic Exposure: A Study by Secondary Ion Mass Spectrometry Microscopy

et al. 2012

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“…SIMS is the most sensitive surface analysis technique, able to detect the presence of elements in the range of parts per billion. Its use in the biomedical field has developed progressively, and a few studies have recently described uranium detection in biological samples with this technique , .…”

Section: Introductionmentioning

confidence: 99%

Distribution of Soluble Uranium in the Nuclear Cell Compartment at Subtoxic Concentrations

Rouas

Bensoussan

Suhard

et al. 2010

Chem. Res. Toxicol.

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Uranium is naturally found in the environment, and its extensive use results in an increased risk of human exposure. Kidney cells have mainly been used as in vitro models to study effects of uranium exposure, and very little about the effects on other cell types is known. The aim of this study was to assess the impact of depleted uranium exposure at the cellular level in human kidney (HEK-293), liver (HepG2), and neuronal (IMR-32) cell lines. Cytotoxicity studies showed that these cell lines reacted in a roughly similar manner to depleted uranium exposure, responding at a cytotoxicity threshold of 300-500 μM. Uranium was localized in cells with secondary ion mass spectrometry technology. Results showed that uranium precipitates at subtoxic concentrations (>100 μM). With this approach, we were able for the first time to observe the soluble form of uranium in the cell at low concentrations (10-100 μM). Moreover, this technique allows us to localize it mainly in the nucleus. These innovative results raise the question of how uranium penetrates into cells and open new perspectives for studying the mechanisms of uranium chemical toxicity.

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Ex vivo decrease in uranium diffusion through intact and excoriated pig ear skin by a calixarene nanoemulsion

Spagnul¹,

Bouvier‐Capely²,

Phan³

et al. 2011

European Journal of Pharmaceutics and Biopharmaceutics

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Detection and analysis of the microdistribution of uranium in the gills of freshwater Corbicula fluminea by SIMS technique

Cited by 3 publications

References 30 publications

Uranium Microdistribution in Renal Cortex of Rats after Chronic Exposure: A Study by Secondary Ion Mass Spectrometry Microscopy

Uranium Microdistribution in Renal Cortex of Rats after Chronic Exposure: A Study by Secondary Ion Mass Spectrometry Microscopy

Distribution of Soluble Uranium in the Nuclear Cell Compartment at Subtoxic Concentrations

Ex vivo decrease in uranium diffusion through intact and excoriated pig ear skin by a calixarene nanoemulsion

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