Ex vivo decrease in uranium diffusion through intact and excoriated pig ear skin by a calixarene nanoemulsion

Spagnul, Aurélie; Bouvier‐Capely, Céline; Phan, Guillaume; Landon, Géraldine; Tessier, Christine; Suhard, David; Rebière, François; Agarande, Michelle; Fattal, Elias

doi:10.1016/j.ejpb.2011.05.004

Cited by 25 publications

(18 citation statements)

References 31 publications

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“…Prior to this study, we designed a calixarene nanoemulsion and demonstrated its ability to remove uranium from skin after artificial contamination . In this study, we aimed to demonstrate the potentiality of this formulation for preventing the diffusion of uranium through the skin on ex vivo models after generating different types of superficial wounds.…”

Section: Discussionmentioning

confidence: 99%

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Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion

Grives

Phan

Morat

et al. 2015

Journal of Pharmaceutical Sciences

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Section: Discussionmentioning

confidence: 99%

“…As a result, an oil‐in‐water (O/W) nanoemulsion loaded with calixarene molecules was designed. This nanoemulsion loaded with a specific chelating agent was shown to be highly efficient in avoiding deep skin penetration of uranium on ex vivo models in Franz diffusion cells, on intact and excoriated pig ear skins …”

Section: Introductionmentioning

confidence: 99%

Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion

Grives

Phan

Morat

et al. 2015

Journal of Pharmaceutical Sciences

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“…Monitoring of Gulf War veterans with uranium‐containing shrapnel embedded in their skin has determined that the shrapnel dissolves and the uranium migrates to systemic organs and is excreted in urine (Leggett and Pellmar, ; McDiarmid et al ., ). Dermal exposure to uranium and other nuclides remains a major concern in the nuclear industry (Phan et al ., ; Spagnul et al ., ; Weber et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Synergistic cytotoxicity and DNA strand breaks in cells and plasmid DNA exposed to uranyl acetate and ultraviolet radiation

Wilson

Zuniga

Yazzie

et al. 2014

J of Applied Toxicology

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Depleted uranium (DU) has a chemical toxicity that is independent of its radioactivity. The purpose of this study was to explore the photoactivation of uranyl ion by ultraviolet (UV) radiation as a chemical mechanism of uranium genotoxicity. The ability of UVB (302 nm) and UVA (368 nm) radiation to photoactivate uranyl ion to produce single strand breaks was measured in pBR322 plasmid DNA, and the presence of adducts and apurinic/apyrimidinic sites that could be converted to single strand breaks by heat and piperidine was analyzed. Results showed that DNA lesions in plasmid DNA exposed to UVB- or UVA-activated DU were only slightly heat reactive, but were piperidine sensitive. The cytotoxicity of UVB-activated uranyl ion was measured in repair-proficient and repair-deficient Chinese hamster ovary cells and human keratinocyte HaCaT cells. The cytotoxicity of co-exposures of uranyl ion and UVB radiation was dependent on the order of exposure and was greater than co-exposures of arsenite and UVB radiation. Uranyl ion and UVB radiation were synergistically cytotoxic in cells, and cells exposed to photoactivated DU required different DNA repair pathways than cells exposed to non-photoactivated DU. This study contributes to our understanding of the DNA lesions formed by DU, as well as their repair. Results suggest that excitation of uranyl ion by UV radiation can provide a pathway for uranyl ion to be chemically genotoxic in populations with dermal exposures to uranium and UV radiation, which would make skin an overlooked target organ for uranium exposures.

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“…However, a closer look at these articles reveals that the systems in question are exclusively dealing with thermodynamically stable microemulsion phases. The versatile nature of nanoemulsions is highlighted by another recent study by Spagnul et al, who designed a calixarene nanoemulsion aimed at chelating uranium molecules at the skin surface (Spagnul et al 2011 ). To allow for the passage of intact nanovectors across pores of the skin, much smaller and deformable carriers are required since the skin is a strong and complex barrier (Cevc and Vierl 2010 ).…”

Section: Dermal Application: Advantages and Limitationsmentioning

confidence: 99%

Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement

Dragićević¹,

Maibach²

2015

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Ex vivo decrease in uranium diffusion through intact and excoriated pig ear skin by a calixarene nanoemulsion

Cited by 25 publications

References 31 publications

Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion

Ex Vivo Uranium Decontamination Efficiency on Wounded Skin and In Vitro Skin Toxicity of a Calixarene-Loaded Nanoemulsion

Synergistic cytotoxicity and DNA strand breaks in cells and plasmid DNA exposed to uranyl acetate and ultraviolet radiation

Percutaneous Penetration Enhancers Chemical Methods in Penetration Enhancement

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