Detecting Tomato Leaf Curl New Delhi Virus Causing Ridge Gourd Yellow Mosaic Disease, and Other Begomoviruses by Antibody-Based Methods

Naganur, Priya; Shankarappa, K. S.; Mesta, R. K.; Rao, C. Durga; Venkataravanappa, Venkataravanappa; Maruthi, M. N.; Reddy, Lakshminarayana Reddy C. Narasimha

doi:10.3390/plants12030490

Cited by 2 publications

(2 citation statements)

References 39 publications

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“…Given Begomoviral CP vital importance, both in terms of their infectivity and antibody-based resistance [ 42 , 43 , 44 , 45 , 46 ], we felt a serious need for the analysis to evaluate its evolution. Thus, our primary objective was to identify and describe the patterns of mutations that indicate positive selection in the coat protein (CP) of both OW and NW viruses, as well as to assess how these patterns change over different geographical regions.…”

Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis of Old World and New World TYLCV Reveals a Biasness toward Highly Variable Amino Acids in Coat Protein

Nigam

Muthukrishnan

Flores-López

et al. 2023

Plants

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Begomoviruses, belonging to the family Geminiviridae and the genus Begomovirus, are DNA viruses that are transmitted by whitefly Bemisia tabaci (Gennadius) in a circulative persistent manner. They can easily adapt to new hosts and environments due to their wide host range and global distribution. However, the factors responsible for their adaptability and coevolutionary forces are yet to be explored. Among BGVs, TYLCV exhibits the broadest range of hosts. In this study, we have identified variable and coevolving amino acid sites in the proteins of Tomato yellow leaf curl virus (TYLCV) isolates from Old World (African, Indian, Japanese, and Oceania) and New World (Central and Southern America). We focused on mutations in the coat protein (CP), as it is highly variable and interacts with both vectors and host plants. Our observations indicate that some mutations were accumulating in Old World TYLCV isolates due to positive selection, with the S149N mutation being of particular interest. This mutation is associated with TYLCV isolates that have spread in Europe and Asia and is dominant in 78% of TYLCV isolates. On the other hand, the S149T mutation is restricted to isolates from Saudi Arabia. We further explored the implications of these amino acid changes through structural modeling. The results presented in this study suggest that certain hypervariable regions in the genome of TYLCV are conserved and may be important for adapting to different host environments. These regions could contribute to the mutational robustness of the virus, allowing it to persist in different host populations.

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Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis of Old World and New World TYLCV Reveals a Biasness toward Highly Variable Amino Acids in Coat Protein

Nigam

Muthukrishnan

Flores-López

et al. 2023

Plants

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“…Due to its ease of use, sensitivity, accuracy, and affordability, serological techniques are widely used such as the different ELISA immnoassays and the Lateral Flow Assay (LFA) has been frequently used for standard virus detection. Naganur et al, 2023). Nucleic acid hybridization are also commonly used for the detection of ToLCNDV, the technique is economical, and allows the processing of a large number of samples, carrying out field surveys to determine the presence of ToLCNDV Hameed et al, 2017;.…”

Section: Diagnostic Methodsmentioning

confidence: 99%

First report of Cucurbit Chlorotic Yellows Virus (CCYV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Algeria and lack of evidence for seed transmission of ToLCNDV in melon and pumpkin.

Kheireddine

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Emerging plant viruses in the Mediterranean Basin have arisen in the last decades becoming an important threat to crop production in this region. The spread of new viral diseases has become one of the main factors limiting agronomic and economic yields of cultivated species of Cucurbitaceae family, the second in importance after Solanaceae. A wide cluster of elements modulates and favors the emergence of these pathogens, including biological, ecological, social, and cultural factors. Global warming favors vector movement to new geographies, while global commercial networks lead to germplasm trade among countries, allowing the long-distance dissemination of viruses. Moreover, the intensive agricultural model, in which prevail genetically homogeneous cultivars, prompts pathogens outbreaks within new geographic areas at a fast rate.One of the key components to develop control strategies, and secure horticultural production, is to identify the type and distribution of viruses present in crops. However, such information in Algeria is scarce and very limited. The objective of this Doctoral Thesis was to identify and determine the prevalence and distribution of the most common viruses on cultivated cucurbit plants (zucchini, cucumber, and melon), and get information about their genetic variability and mode of dispersion.In this work, cucurbit chlorotic yellows virus (CCYV) was first detected infecting cucumber and zucchini in Algeria. CCYV (genus Crinivirus, family Closteroviridae) is part of a complex of whitefly-transmitted viruses that cause yellowing disease in cucurbits. Determination of the complete CP, and partial RdRp and Hsp70 sequences of an Algerian CCYV isolate was conducted to unveil the evolutionary relationships with the published isolates in databases. The phylogenetic analysis showed that the Algerian isolate clustered into group I together with the majority of the reported CCYV isolates. The genetic variation within this group was very low (nucleotide identity higher than 99.5%) despite their extensive and discontinuous geographical distribution, plant species (cultivated or weeds) and year of collection. This low genetic divergence could be due to the recentness of this virus that did not face major changes for the present time. In our case, the presence of CCYV was only detected in mixed infection with tomato leaf curl New Delhi virus (ToLCNDV). In future works, it will be interesting to check the likely synergistic or antagonistic interaction between these two viruses that may affect the symptoms development and spread between crops. SUMMARY viiiToLCNDV (genus Begomovirus, family Geminiviridae) was also first detected infecting cucurbit plants in Algeria. ToLCNDV is a bipartite begomovirus that causes destructive epidemics on economically valuable cucurbit and solanaceous crops. The complete DNA-A and DNA-B genomic sequences of a ToLCNDV isolate from cucumber (ToLCNDV-Biskra) were obtained.Alignments of the nucleotide sequences of DNA-A and DNA-B revealed identities of 98.7 and 97.6%, respectively, ...

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Detecting Tomato Leaf Curl New Delhi Virus Causing Ridge Gourd Yellow Mosaic Disease, and Other Begomoviruses by Antibody-Based Methods

Cited by 2 publications

References 39 publications

Comparative Genome Analysis of Old World and New World TYLCV Reveals a Biasness toward Highly Variable Amino Acids in Coat Protein

Comparative Genome Analysis of Old World and New World TYLCV Reveals a Biasness toward Highly Variable Amino Acids in Coat Protein

First report of Cucurbit Chlorotic Yellows Virus (CCYV) and Tomato Leaf Curl New Delhi Virus (ToLCNDV) in Algeria and lack of evidence for seed transmission of ToLCNDV in melon and pumpkin.

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