Detecting the Adsorption of Dye Molecules in Homogeneous Poly(propylene imine) Dendrimer Monolayers by Surface Plasmon Resonance Sensor

Chen, Shengfu; Yu, Qiuming; Li, Lingyan; Boozer, Christina; Homola, Jiřı́; Yee, Sinclair S.; Jiang, Shaoyi

doi:10.1021/ja0170201

Cited by 38 publications

(19 citation statements)

References 34 publications

(61 reference statements)

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“…SPR was performed with an instrument developed at the University of Washington. 29 Generic SPR chips were prepared by coating a glass substrate with an adhesion-promoting chromium layer ͑2 nm͒ and a surfaceplasmon-active gold layer ͑50 nm͒ using electron beam evaporation under vacuum. To perform SPR experiments, a plasma-deposited or bare gold chip was attached to the base of the prism, and optical contact was established using a refractive index matching fluid ͑Cargille Laboratories, Cedar Grove, NJ͒.…”

Section: E Surface Plasmon Resonance "Spr…mentioning

confidence: 99%

Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide

et al. 2006

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Thorough studies of protein interactions with stimulus responsive polymers are necessary to provide a better understanding of their applications in biosensors and biomaterials. In this study, protein behavior on a thermoresponsive polymer surface, plasma polymerized N-isopropyl acrylamide (ppNIPAM), is investigated using multiple characterization techniques above and below its lower critical solution temperature (LCST). Protein adsorption and binding affinity are probed using radiolabeled proteins. Protein activity is estimated by measuring the immunological activity of an antibody adsorbed onto ppNIPAM using surface plasmon resonance. Conformation/orientation of the proteins is probed by time-of-flight secondary ion mass spectrometry (TOF-SIMS) and principal component analysis (PCA) of the TOF-SIMS data. In this work, we find that at low protein solution concentrations, ppNIPAM-treated surfaces are low fouling below the LCST, but protein retentive above it. The protein adsorption isotherms demonstrate that apparent affinity between soluble protein molecules and the ppNIPAM surface are an order of magnitude lower at room temperature than at 37 °C. Although direct protein desorption is not observed in our study when the surface temperature drops below the LCST, the binding affinity of surface adsorbed protein with ppNIPAM is reduced, as judged by a detergent elution test. Furthermore, we demonstrated that proteins adsorbed onto ppNIPAM are functionally active, but the activity is better preserved at room temperature than 37 °C. The temperature dependent difference in protein activity as well as TOF-SIMS and PCA study suggest that proteins take different conformations/orientations after adsorption on ppNIPAM above and below the LCST.

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Section: E Surface Plasmon Resonance "Spr…mentioning

confidence: 99%

Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide

et al. 2006

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“…It shows the limit of detection of 110 ppt for TNT. The interaction of dye molecules with the fifth-generation PPI dendrimer that is immobilized on a gold-coated glass substrate was studied by Jiang [109] by using an SPR sensor. This dendrimer is shown to have high affinity to Rose Bengal and erythrosine B, probably due to their good match with the charge and shape of the cavities of the dendrimer.…”

Section: Other Sensors Using Dendrimersmentioning

confidence: 99%

Dendrimer‐Based Sensors

2011

Chemosensors

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“…[11] Despite, the several high tech. applications of dendrimers like chemical sensors, [12,13] catalysts, [14] molecular devices [15,16] and chemodelivery in biology, [17] these new classes of materials have failed to make any commercial impact. Moreover, as compared with organic hyperbranched polymers and dendrimers, inorganic hyperbranched polymers and dendrimers have been less studied.…”

Section: Introductionmentioning

confidence: 99%

Studies on the synthesis and thermal properties of alkoxysilane‐terminated organosilicone dendrimers

Saxena

Bisaria

Saxena

2010

Applied Organom Chemis

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Silicone core dendrimers bearing terminal dialkoxy and trialkoxy silane groups were prepared in a three-step synthesis. Initially, the Si-H terminated multifunctional silicone dendrimer, i.e. tetrakis(dimethylsiloxy)silane, was prepared by the reaction of tetraethoxysilane and dimethylethoxysilane. Tetrakis(dimethylsiloxy)silane on reaction with allylglycidylether in the presence of Speier's catalyst under pressure (100 psi) yielded epoxy-terminated dendrimer in very high yield (95%). The epoxy-terminated dendrimer was reacted with aminopropylalkoxysilanes to yield the next-generation dendrimer bearing dialkoxy and trialkoxy silane groups. The dendrimers were characterized by the usual physico-chemical techniques, i.e. elemental analysis, FT-IR, 1 H, 13 C and 29 Si NMR. Thermal studies (Thermogravimetric analysis and Thermomechanical analysis) of the alkoxy terminated dendrimers and its cured products were also carried out.

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Detecting the Adsorption of Dye Molecules in Homogeneous Poly(propylene imine) Dendrimer Monolayers by Surface Plasmon Resonance Sensor

Cited by 38 publications

References 34 publications

Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide

Temperature dependent activity and structure of adsorbed proteins on plasma polymerized N-isopropyl acrylamide

Dendrimer‐Based Sensors

Studies on the synthesis and thermal properties of alkoxysilane‐terminated organosilicone dendrimers

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