Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test

Linn, Alexander I.; Košnarová, Pavlína; Soukup, Josef; Gerhards, Roland

doi:10.17221/110/2018-pse

Cited by 4 publications

(2 citation statements)

References 21 publications

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“…Apera spica‐venti resistance was confirmed against acetolactate‐synthase (ALS), ACCase, and photosystem II inhibitors mainly in Germany, Denmark, the Czech Republic, and Poland 1 . Also, its resistance against pinoxaden was reported before 15,21 . TSR mutations in the ACCase sequence were present in low frequencies in the populations resistant to pinoxaden, therefore, metabolic detoxification was indicated to be the significant cause of resistance 22 .…”

Section: Introductionmentioning

confidence: 99%

“…1 Also, its resistance against pinoxaden was reported before. 15,21 TSR mutations in the ACCase sequence were present in low frequencies in the populations resistant to pinoxaden, therefore, metabolic detoxification was indicated to be the significant cause of resistance. 22 Still, the knowledge concerning the NTSR mechanism to this herbicide at the molecular level is deficient.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis indicates the involvement of herbicide‐responsive and plant–pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica‐venti

Wrzesińska‐Krupa

Szmatoła

Praczyk

et al. 2023

Pest Management Science

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Background: The continuous use of the herbicides contributes to the emergence of the resistant populations of numerous weed species that are tolerant to multiple herbicides with different modes of action (multiple resistance) which is provided by nontarget-site resistance mechanisms. In this study, we addressed the question of rapid acquisition of herbicide resistance to pinoxaden (acetyl CoA carboxylase inhibitor) in Apera spica-venti, which endangers winter cereal crops and has high adaptation capabilities to inhabit many rural locations. To this end, de novo transcriptome of Apera spica-venti was assembled and RNAsequencing analysis of plants resistant and susceptible to pinoxaden treated with this herbicide was performed.Results: The obtained data showed that the prime candidate genes responsible for herbicide resistance were those encoding 3-ketoacyl-CoA synthase 12-like, UDP-glycosyltransferases (UGT) including UGT75K6, UGT75E2, UGT83A1-like, and glutathione S-transferases (GSTs) such as GSTU1 and GSTU6. Also, such highly accelerated herbicide resistance emergence may result from the enhanced constitutive expression of a wide range of genes involved in detoxification already before herbicide treatment and may also influence response to biotic stresses, which was assumed by the detection of expression changes in genes encoding defence-related proteins, including receptor kinase-like Xa21. Moreover, alterations in the expression of genes associated with methylation in non-treated herbicide-resistant populations were identified. Conclusion:The obtained results indicated genes that may be involved in herbicide resistance. Moreover, they provide valuable insight into the possible effect of resistance on the weed interaction with the other stresses by indicating pathways associated with both abiotic and biotic stresses.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis indicates the involvement of herbicide‐responsive and plant–pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica‐venti

Wrzesińska‐Krupa

Szmatoła

Praczyk

et al. 2023

Pest Management Science

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Detection of Resistance in Echinochloa spp. to Three Post-Emergence Herbicides (Penoxsulam, Metamifop, and Quinclorac) Used in China

Zhang

Bao

et al. 2023

Agronomy

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In this study, rapid resistance in-season quick (RISQ) tests were developed for detecting the resistance in Echinochloa spp. to penoxsulam, metamifop, and quinclorac, which are widely used in rice fields to control E. spp. biotypes. Seedlings in 1–2 leaf stages from nine biotypes of E. crusgalli, E. crusgalli var. zelayensis, and E. glabrescens, with different susceptibility to the three herbicides tested, were transplanted to plates containing nutrient agar and different rates of herbicides. The survival rates were recorded at 8 days after treatment when no more new roots emerged for all the treatments. By comparing the results from RISQ tests and whole-plant pot bioassays statistically, discrimination rates could be determined to distinguish resistant plants from susceptible plants. For penoxsulam, metamifop, and quinclorac, the discrimination rates were 0.3, 0.6, and 2.4 µmol/L, respectively. Two additional biotypes of E. crusgalli collected in rice fields were used to confirm the validation of the RISQ test and the obtained results by the RISQ test were consistent with that of the whole-plant pot bioassay. Therefore, the developed RISQ test would be a possible alternative method to determine the susceptibility of E. spp. to certain herbicides.

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Molecular Mechanisms of Resistance against PSII-Inhibiting Herbicides in Amaranthus retroflexus from the Czech Republic

Mikulka,

Sen,

Košnarová

et al. 2024

Genes

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Amaranthus retroflexus L. (redroot pigweed) is one of the most problematic weeds in maize, sugar beet, vegetables, and soybean crop fields in Europe. Two pigweed amaranth biotypes (R1 and R2) from the Czech Republic resistant to photosystem II (PSII)-inhibiting herbicides were analyzed in this study. This study aimed to identify the genetic mechanisms that underlie the resistance observed in the biotypes. Additionally, we also intended to establish the use of chlorophyll fluorescence measurement as a rapid and reliable method for confirming herbicide resistance in this weed species. Both biotypes analyzed showed high resistance factors in a dose–response study and were thus confirmed to be resistant to PSII-inhibiting herbicides. A sequence analysis of the D1 protein revealed a well-known Ser-Gly substitution at amino acid position 264 in both biotypes. Molecular docking studies, along with the wild-type and mutant D1 protein’s secondary structure analyses, revealed that the S264G mutation did not reduce herbicide affinity but instead indirectly affected the interaction between the target protein and the herbicides. The current study identified the S264G mutation as being responsible for conferring herbicide resistance in the pigweed amaranth biotypes. These findings can provide a strong basis for future studies that might use protein structure and mutation-based approaches to gain further insights into the detailed mechanisms of resistance in this weed species. In many individuals from both biotypes, resistance at a very early stage (BBCH10) of plants was demonstrated several hours after the application of the active ingredients by the chlorophyll fluorescence method. The effective PS II quantum yield parameter can be used as a rapid diagnostic tool for distinguishing between sensitive and resistant plants on an individual level. This method can be useful for identifying herbicide-resistant weed biotypes in the field, which can help farmers and weed management practitioners develop more effective weed control tactics.

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Detecting herbicide-resistant Apera spica-venti with a chlorophyll fluorescence agar test

Cited by 4 publications

References 21 publications

Transcriptome analysis indicates the involvement of herbicide‐responsive and plant–pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica‐venti

Transcriptome analysis indicates the involvement of herbicide‐responsive and plant–pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica‐venti

Detection of Resistance in Echinochloa spp. to Three Post-Emergence Herbicides (Penoxsulam, Metamifop, and Quinclorac) Used in China

Molecular Mechanisms of Resistance against PSII-Inhibiting Herbicides in Amaranthus retroflexus from the Czech Republic

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