Detecting early bone changes using in vivo micro-CT in ovariectomized, zoledronic acid-treated, and sham-operated rats

Perilli, Egon; Le, V.; Ma, Bingkui; Salmon, Phil; Reynolds, Karen; Fazzalari, Nicola L.

doi:10.1007/s00198-009-1082-z

Cited by 63 publications

(54 citation statements)

References 41 publications

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“…This is likely according to the effects of ZOL in the first 2 weeks of the injection mainly preserved and restored the cancellous bone microarchitecture to achieve the microarchitectural structures and patterns similar to the control group. 34 The results of present study are also in agreement with our previous study 21 in which ovariectomized rats showed an increase in orthodontic tooth movement and OIRR via the bone metabolism pathways resulting from hormonal imbalance. Moreover, the present study might have the important implications for the future osteoporosis studies especially in the experimental orthodontic tooth movement and OIRR because the results of the first 2 weeks post-OVX in the rats could be a reference time point to achieve the effective recovery of the bone volume by injection of ZOL.…”

Section: Discussionsupporting

confidence: 93%

“…28,42,43 Recently, a study has elucidated that after 4 weeks of OVX (only 2 weeks after the injection of ZOL was started), the OVX group has shown the loss of the bone volume fraction in 64 %, whereas the OVX+ZOL group has shown the bone volume fraction similarly to the baseline. 34 In our study, we started to apply orthodontic force 4 weeks after OVX and we found almost the same results of both orthodontic tooth movement and OIRR in OVX+ZOL and control groups.…”

Section: Discussionsupporting

confidence: 61%

“…33 Sham operations were also performed in the control group, in which all of the other procedures were exactly the same, except for the removal of the ovaries. Two weeks after OVX, ZOL (Zometa, Novartis, Basel, Switzerland) was injected into the peritoneal cavity (1.6 μg/kg) of the OVX+ZOL group 34 , and continued once a week totally 6 times. The last dose was injected on the same day coinciding with day 21 of the experimental orthodontic tooth movement (Fig 1 A).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid

Sirisoontorn

Hotokezaka

Hashimoto

et al. 2012

American Journal of Orthodontics and Dentofacial Orthopedics

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 61%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid

Sirisoontorn

Hotokezaka

Hashimoto

et al. 2012

American Journal of Orthodontics and Dentofacial Orthopedics

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“…Although it has been used in spine studies previously, it has never been used to study in Modic changes before [30][31][32]. It is typically used for ex vivo scanning of excised human and animal bones [14,29] or in vivo scanning of small animals, at micrometer resolution [33]. For clinical CT scans on human patients in vivo, the improvement in spatial resolution (decrease of pixel size) is currently limited by the corresponding increase in radiation dose [28].…”

Section: Discussionmentioning

confidence: 99%

Modic (endplate) changes in the lumbar spine: bone micro-architecture and remodelling

et al. 2014

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Purpose In the literature, inter-vertebral MRI signal intensity changes (Modic changes) were associated with corresponding histological observations on endplate biopsies. However, tissue-level studies were limited. No quantitative histomorphometric study on bone biopsies has yet been conducted for Modic changes. The aim of this study was to characterise the bone micro-architectural parameters and bone remodelling indices associated with Modic changes. Methods Forty patients suffering from disabling low back pain, undergoing elective spinal surgery, and exhibiting Modic changes on MRI (Modic 1, n = 9; Modic 2, n = 25; Modic 3, n = 6), had a transpedicular vertebral body biopsy taken of subchondral bone. Biopsies were first examined by micro-CT, for 3D morphometric analysis of bone volume fraction (BV/TV), trabecular thickness (Tb.Th), trabecular separation, trabecular number, and structure model index. Then, samples underwent histological analysis, for determination of bone remodelling indices: osteoid surface to bone surface ratio (OS/BS), eroded surface to bone surface (ES/BS) and osteoid surface to eroded surface ratio (OS/ES). Results Micro-CT analysis revealed significantly higher BV/TV (up to 70 % increase, p \ 0.01) and Tb.Th (up to ?57 %, p \ 0.01) in Modic 3 biopsies, compared to Modic 1 and 2. Histological analysis showed significantly lower OS/BS in Modic 2 biopsies (more than 28 % decrease, p \ 0.05) compared to 1 and 3. ES/BS progressively decreased from Modic 1 to 2 to 3, whereas OS/ES progressively increased with significantly higher values in Modic 3 (up to 159 % increase, p \ 0.05) than in Modic 1 and 2. Conclusions Significant differences were found in bone micro-architectural parameters and remodelling indices among Modic types. Modic 1 biopsies had evidence of highest bone turnover, possibly due to an inflammatory process; Modic 2 biopsies were consistent with a reduced bone formation/remodelling stage; Modic 3 biopsies suggested a more stable sclerotic phase, with significantly increased BV/TV and Tb.Th compared to Modic 1 and 2, linked to increased bone formation and reduced resorption.

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“…Perilli et al 11 reported that treatment with ZOL as late as 2 weeks after ovariectomy (OVX) can still facilitate complete reversal of cancellous bone loss in rat tibia. Sirisoontorn et al 12 recently reported that OVX accelerated and ZOL inhibited OTM in rats.…”

mentioning

confidence: 99%

The effect of bone morphometric changes on orthodontic tooth movement in an osteoporotic animal model

Hashimoto

Hotokezaka

Sirisoontorn

et al. 2013

The Angle Orthodontist

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Objective: To elucidate the effect of bone morphometric changes on orthodontic tooth movement (OTM) in zoledronic acid-treated ovariectomized rats. Materials and Methods: Twenty-one 10-week-old female Wistar rats were divided into ovariectomy (OVX), OVX with zoledronic acid administration (OVX + ZOL), and sham operation (control) groups. Two weeks after OVX, ZOL administration was initiated. Twelve weeks after OVX, a nickel-titanium closed-coil spring of 25-g force was applied mesially to the maxillary left first molar. In vivo micro-computed tomography (CT) of the left proximal tibia was performed for bone morphometric analysis every 2 weeks after OVX. In addition, OTM was investigated using micro-CT at 0, 12, and 14 weeks after OVX. Results: There were significant differences in the bone mineral content (BMC), bone volume (BV), BMC to tissue volume ratio (BMC/TV), and BV to TV ratio of trabecular bone between the control and OVX groups and also between the OVX + ZOL and OVX groups. In the OVX + ZOL group, increased BMC and BV in the cortical bone and increased bone mineral density (BMD) in the trabecular bone were observed. Interestingly, OTM in the OVX group was almost two times more than that in the control and OVX + ZOL groups. Moreover, OTM was correlated with BMD, BMC, BV, and BMC/TV in the trabecular bone. Conclusions: OVX accelerated OTM, while ZOL suppressed it. OTM demonstrated a significant negative relationship with trabecular bone mass. (Angle Orthod. 2013;83:766-773.)

show abstract

Detecting early bone changes using in vivo micro-CT in ovariectomized, zoledronic acid-treated, and sham-operated rats

Abstract: Treatment with zoledronic acid as late as 2 weeks after ovariectomy still facilitates the full reversal of cancellous bone loss in the rat tibia.

Cited by 63 publications

References 41 publications

Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid

Orthodontic tooth movement and root resorption in ovariectomized rats treated by systemic administration of zoledronic acid

Modic (endplate) changes in the lumbar spine: bone micro-architecture and remodelling

The effect of bone morphometric changes on orthodontic tooth movement in an osteoporotic animal model

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