Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP)

Liu, Aihong; Guan, Guiquan; Liu, Zhijie; Liu, Junlong; LeBlanc, Neil; Li, Youquan; Gao, Jinliang; Ma, Miling; Niu, Qinli; Ren, Qiaoyun; Bai, Quan; Yin, Hong; Luo, Jianxun

doi:10.1016/j.exppara.2010.05.024

Cited by 57 publications

(34 citation statements)

References 19 publications

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“…This observation is in agreement with previous studies on the analyses of the 18S rRNA gene and MSPS gene sequences (Chansiri et al, 1999;Gubbels et al, 2000;Yin et al 2004;Liu et al, 2010b After studying the morphology, vector and phylogenetic relationship of this novel genotype with other Theileria spp., this genotype was found to be a distinct species and was designated as T.…”

Section: Identification Of T Buffeli-like and T Sinensis-like 18s Rsupporting

confidence: 81%

“…Together with T. annulata, T. sinensis and T. sergenti are the causative agents of bovine theiloriosis in China (Liu et al, 2010b). Morphologically, T. sinensis and T. sergenti are indistinguishable , but they have different tick vectors as T. sergenti is transmitted by Haemaphysalis longicornis (Liu et al, 2010b).…”

Section: Identification Of T Buffeli-like and T Sinensis-like 18s Rmentioning

confidence: 99%

“…Morphologically, T. sinensis and T. sergenti are indistinguishable , but they have different tick vectors as T. sergenti is transmitted by Haemaphysalis longicornis (Liu et al, 2010b). Liu et al (2010b) developed a PCR assay, based on MPSP gene sequences, for the detection and discrimination of these two species from cattle and yak.…”

Section: Identification Of T Buffeli-like and T Sinensis-like 18s Rmentioning

confidence: 99%

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Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Chaisi

Collins

Oosthuizen

2014

Veterinary Parasitology

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Theileria buffeli/T. sergenti/T. orientalis is a group of benign and mildly pathogenic species of cattle and buffalo. In a previous study, we identified T .buffeli in blood samples originating from the African buffalo (Syncerus caffer) in the Hluhluwe-iMfolozi Game Park (HIP) and the Addo Elephant Game Park (AEGP) in South Africa. The aim of this study was to characterize the 18S rRNA gene and complete internal transcribed spacer (ITS1-5.8S-ITS2) region of T. buffeli samples, and to establish the phylogenetic position of this species based on these loci. The 18S rRNA gene and the complete ITS region were amplified from DNA extracted from blood samples originating from buffalo in these localities. The PCR products were cloned and the resulting recombinants sequenced. We identified novel T. buffeli 18S rRNA gene and ITS genotypes from buffalo in the AEGP, and novel T. sinensis 18S rRNA genotypes from buffalo in the HIP. Phylogenetic analyses indicated that the T. buffeli-like sequences were similar to T. buffeli sequences from cattle and buffalo in China and India, and the T. sinensis-like sequences were similar to T. sinensis 18S rRNA sequences of cattle and yak in China. There was extensive sequence variation between the novel T.1 buffeli genotypes of the African buffalo and previously described T. buffeli and T. sinensis genotypes. The presence of organisms with T. buffeli-like and T. sinensis-like genotypes in the African buffalo could be of significant importance, particularly to the cattle industry in South Africa as these animals might act as sources of infections to naïve cattle. This is the first report on the characterization of the full-length 18S rRNA gene and ITS region of T. buffeli and T. sinensis genotypes in South Africa. Our study provides invaluable information towards the classification of this complex group of benign and mildly pathogenic species.

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Section: Identification Of T Buffeli-like and T Sinensis-like 18s Rsupporting

confidence: 81%

Section: Identification Of T Buffeli-like and T Sinensis-like 18s Rmentioning

confidence: 99%

Section: Identification Of T Buffeli-like and T Sinensis-like 18s Rmentioning

confidence: 99%

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Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Chaisi

Collins

Oosthuizen

2014

Veterinary Parasitology

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“…Infection by T. sinensis is widespread among cattle and yaks throughout China, and Lintao, Dingxi, and Weiyuan city from Gansu Province were described as endemic regions of T. sinensis (Yin et al, 2002;Liu et al, 2010). Here, T. sinensis infections were present in Linzhou of Henan province with prevalence (2.9%) comparatively lower than previous reports .…”

Section: Discussioncontrasting

confidence: 47%

Identification of 12 Piroplasms Infecting Ten Tick Species in China Using Reverse Line Blot Hybridization

Abdallah

Niu

Yang

et al. 2017

Journal of Parasitology

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“…T. orientalis is a member of the generally benign Theileria group (T. orientalis/sergenti/buffeli), which is locally known as T. sergenti in China, Japan, Korea and Russia [8,15]. T. sergenti is an invalid name from a taxonomic viewpoint, since it has been used to previously describe a parasite of sheep [9,16]. Recently, some genotypes of T. orientalis that affected cattle have been identified in some countries, such as China [10], Thailand [13], Vietnam [7] and Japan [20].…”

mentioning

confidence: 99%

Molecular Detection of <i>Theileria</i> Species in Sheep from Northern China

Cao

Shou-fa

Jia

et al. 2013

The Journal of Veterinary Medical Science

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ABSTRACT. Ovine theileriosis is a tick-borne disease that restricts the development of small ruminant husbandry in northern China. In this study, we report on a molecular epidemiological survey of ovine Theileria spp. in 198 blood samples taken from sheep in northern China. The DNA samples were screened by a nested polymerase chain reaction (PCR) targeting the 18S rRNA gene of ovine Theileria spp. The prevalence of ovine Theileria spp. in Yanji, Nongan, Longjing, Toudao and Jinchang was 80%, 40%, 37%, 24% and 32%, respectively. The sequencing analyses approved the present of the T. orientalis and/or T. luwenshuni in these regions. Taken together, we have demonstrated a high incidence of Theileria spp. in northern China that calls for the need to design effective control programs for ovine theileriosis.

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Detecting and differentiating Theileria sergenti and Theileria sinensis in cattle and yaks by PCR based on major piroplasm surface protein (MPSP)

Cited by 57 publications

References 19 publications

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Phylogeny of Theileria buffeli genotypes identified in the South African buffalo (Syncerus caffer) population

Identification of 12 Piroplasms Infecting Ten Tick Species in China Using Reverse Line Blot Hybridization

Molecular Detection of <i>Theileria</i> Species in Sheep from Northern China

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