Identification of 12 Piroplasms Infecting Ten Tick Species in China Using Reverse Line Blot Hybridization

Abdallah, Mirza Omar; Niu, Qingli; Yang, Jifei; Hassan, Mohammad Farooque; Yu, Peifa; Guan, Guiquan; Chen, Ze; Liu, Guangyuan; Luo, Jianxun; Yin, Hong

doi:10.1645/16-161

Cited by 17 publications

(8 citation statements)

References 38 publications

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“…Recently, Liu et al [ 30 ] detected a new bovine Babesia , similar to B. venatorum and Qin et al [ 31 ] reported an epidemiological study of B. orientalis in China. Among different members of Theileria , T. annulata , T. orientalis and T. sinensis , have been reported to cause the disease in China [ 4 ]. Recently, T. luwenshuni was reported for the first time in yaks [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

“…In general, Theileria annulata and Theileria parva are considered to be the most pathogenic species, causing theileriosis in cattle and buffalo populations of tropical and subtropical areas, whereas Theileria mutans and Theileria orientalis are responsible for asymptomatic disease [ 3 ]. Hard ticks of the genera Hyalomma , Rhipicephalus , Haemaphysalis and Amblyomma are vectors of these Theileria spp., resulting in fatal economic losses in endemic areas of Asia and Africa [ 4 , 5 ]. Clinical manifestations of theileriosis include: increased body temperature (40–41.5 °C), followed by depression, watery secretion from the eyes, nasal discharge, inflammation of the lymph nodes and anemia [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

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Molecular survey of piroplasm species from selected areas of China and Pakistan

Hassan¹,

Liu

Rashid

et al. 2018

Parasites Vectors

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BackgroundPiroplasmosis is an important animal disease that is a major constraint to the development of the livestock industry, often resulting in significant economic losses. Therefore, there is an urgent need to further understand the etiology of this and other tick-borne infections.MethodsBlood samples were collected from tick-infested animals from the Chakwal, Jhang, and Faisalabad districts of Punjab, Pakistan and from peri-urban areas around Hohhot, Inner Mongolia, China to investigate the presence of Babesia and Theileria species. In total, 450 blood samples were collected with FTA cards from cattle of the study areas of Pakistan; the genomic (g)DNA of one hundred and twenty samples from cattle in Inner Mongolia were provided by the Lanzhou Veterinary Research Institute, China. Following the extraction of gDNA, the 18S rRNA gene (V4 hypervariable region) of piroplasms was amplified in all samples using semi-nested PCR. Positively identified samples were sequenced for the identification of Theileria and Babesia species. The partial full-length sequence of 18S rDNA was amplified for species confirmation of Theileria-positive samples, whereas the RAP-1c gene was amplified for Babesia bigemina-positive samples.ResultsSemi-nested PCR results revealed that 144 (25.26%) samples were positive for piroplasms. Theileria annulata was the most prevalent species (115/144; 20.17%), followed by Theileria orientalis (16/144; 2.80%). Among Babesia, the only species recorded was Babesia bigemina (13/144; 2.28%).ConclusionThe present study reveals new data on the prevalence of piroplasm species in bovine populations of selected areas of China and Pakistan and their phylogenetic relationships. It is also the first detailed report of T. orientalis from native animals in Pakistan.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular survey of piroplasm species from selected areas of China and Pakistan

Hassan¹,

Liu

Rashid

et al. 2018

Parasites Vectors

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“…It should be taken into account that the RLB design used in this study was based on the most prevalent vector-borne pathogens present in Argentina. However, the detection potential of the membrane can be easily expanded for other vector borne pathogens present in bovines (in blood or other tissues) or even in the vectors from this and other geographical regions (Abdallah et al, 2017).…”

Section: Tablementioning

confidence: 99%

Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay

Paoletta

Arias

Fourniere

et al. 2018

Ticks and Tick-borne Diseases

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“…Tick infestation also results in significant economic losses in the cattle industry. Piroplasmosis (babesiosis and theileriosis) and rickettsiosis (anaplasmosis) are common protozoan diseases spread by ticks among cattle, and they have become endemic in various countries, causing several health problems in domestic ruminants [5,6]. South Asia houses several tick species; in fact, 97 species have been reported in this region [7].…”

Section: Introductionmentioning

confidence: 99%

Ticks (Acari: Ixodidae) infestation on cattle in various regions in Indonesia

Sahara

Nugraheni

Patra³

et al. 2019

Vet World

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Background and Aim: Ticks (Ixodidae) not only cause blood loss in cattle but also serve as vectors for various diseases, thus causing direct and indirect losses. Moreover, tick infestation can cause significant economic losses. This study aimed to identify the diverse species of ticks infesting cattle in five different regions in Indonesia. Materials and Methods: Tick specimens were obtained from local cattle in five different areas in Indonesia. The morphology of the specimens was macroscopically and microscopically evaluated, and the resulting data were descriptively and qualitatively analyzed. Results: In total, 1575 ticks were successfully collected from 26 animals. In total, two genera and three species, namely, Rhipicephalus microplus, Haemaphysalis bispinosa, and Rhipicephalus pilans, were identified. The cattle in Yogyakarta and Riau were infested by H. bispinosa, while the cattle in Sukabumi, Bali, and Lombok were infested by R. microplus and R. pilans. The level of infestation varied among regions, with R. microplus being the most commonly found species. Conclusion: The results of this study revealed that cattle in different regions of Indonesia were infested by variable numbers of tick species. In particular, the cattle in Yogyakarta and Riau were solely infested by H. bispinosa; this is a new finding in terms of the distribution of tick species in the country. Increased tick infestation in cattle decreases productivity and causes health problems; therefore, it deserves serious attention. Our findings can help in the formulation of an effective strategy for controlling and preventing cattle tick infestation in the country.

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Identification of 12 Piroplasms Infecting Ten Tick Species in China Using Reverse Line Blot Hybridization

Cited by 17 publications

References 38 publications

Molecular survey of piroplasm species from selected areas of China and Pakistan

Molecular survey of piroplasm species from selected areas of China and Pakistan

Epidemiology of Babesia, Anaplasma and Trypanosoma species using a new expanded reverse line blot hybridization assay

Ticks (Acari: Ixodidae) infestation on cattle in various regions in Indonesia

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