Detección Molecular de Estreptococos Cariogénicos en Saliva

Salazar, Luis A.; Vásquez, Claudio C.; Almuna, Alejandro; Oporto, Gonzalo H.; Santana, Roberto; Herrera, Christian L; Sanhueza, Antonio

doi:10.4067/s0717-95022008000400027

Cited by 12 publications

(13 citation statements)

References 17 publications

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“…S. mutans and S. sobrinus were clinical samples obtained from children of La Araucanía Region isolated in a previous study and confirmed by PCR-RFLP ( Salazar et al , 2008 ).…”

Section: Methodsmentioning

confidence: 71%

Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus

Barrientos¹,

Herrera²,

Montenegro³

et al. 2013

Braz. J. Microbiol.

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Propolis is a non-toxic natural substance with multiple pharmacological properties including anti-cancer, antioxidant, fungicidal, antibacterial, antiviral, and anti-inflammatory among others. The aim of this study was to determine the chemical and botanical characterization of Chilean propolis samples and to evaluate their biological activity against the cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus. Twenty propolis samples were obtained from beekeeping producers from the central and southern regions of Chile. The botanical profile was determined by palynological analysis. Total phenolic contents were determined using colorimetric assays. Reverse phase HPLC and HPLC-MS were used to determine the chemical composition. The minimum inhibitory concentration (MIC) was determined on S. mutans and S. sobrinus. All propolis samples were dominated by structures from native plant species. The characterization by HPLC/MS, evidenced the presence of quercetin, myricetin, kaempferol, rutine, pinocembrin, coumaric acid, caffeic acid and caffeic acid phenethyl ester, that have already been described in these propolis with conventional HPLC. Although all propolis samples inhibited the mutans streptococci growth, it was observed a wide spectrum of action (MIC 0.90 to 8.22 μg mL−1). Given that results it becomes increasingly evident the need of standardization procedures, where we combine both the determination of botanical and the chemical characterization of the extracts. Research conducted to date, describes a promising effectiveness of propolis in the prevention of caries and other diseases of the oral cavity, making it necessary to develop studies to identify and understand the therapeutic targets or mechanisms of molecular action of the various compounds present on them.

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“…S. mutans and S. sobrinus were clinical samples obtained from children of La Araucanía Region isolated in a previous study and confirmed by PCR-RFLP ( Salazar et al , 2008 ).…”

Section: Methodsmentioning

confidence: 71%

Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus

Barrientos¹,

Herrera²,

Montenegro³

et al. 2013

Braz. J. Microbiol.

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“…Clinical isolates of Streptococcus mutans were obtained from the bacterial strain collection of our research center and confirmed by PCR as previously described [ 18 ]. Bacteria were grown on Columbia agar plates supplied with sucrose (1%) and incubated in anaerobic atmosphere (Anaerobic Generator GasPak EZ, Becton, Dickinson and Co., NY, USA) at 37°C and for 24 hours.…”

Section: Methodsmentioning

confidence: 99%

Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

Veloz

Saavedra

Lillo

et al. 2015

BioMed Research International

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The chemical composition of propolis varies according to factors that could have an influence on its biological properties. Polyphenols from propolis have demonstrated an inhibitory effect on Streptococcus mutans growth. However, it is not known if different years of propolis collection may affect its activity. We aimed to elucidate if the year of collection of propolis influences its activity on Streptococcus mutans. Polyphenol-rich extracts were prepared from propolis collected in three different years, characterized by LC-MS and quantified the content of total polyphenols and flavonoids groups. Finally, was evaluated the antibacterial effect on Streptococcus mutans and the biofilm formation. Qualitative differences were observed in total polyphenols, flavones, and flavonols and the chemical composition between the extracts, affecting the strength of inhibition of biofilm formation but not the antimicrobial assays. In conclusion, chemical composition of propolis depends on the year of collection and influences the strength of the inhibition of biofilm formation.

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“…Studies using saliva as a source of cariogenic bacteria show that the presence of the microorganisms studied from the mutans group was at 100% 13 . Given that the intention of the studies is to relate the presence of cariogenic bacteria and dental caries to confirm risk in subsequent analytical studies, using saliva as a source of the bacteria studied does not permit establishing an effective association.…”

Section: Discussionmentioning

confidence: 99%

“…Sample size was calculated based on the historical tendency 12,13 from the studies with most similarity with the procedures used in this study. The participants were selected according to the following criteria: children aged between three and five years, residing in the geographical area of the target population of study, officially registered in foster homes of the region, and that parents accepted to participate in the study by signing an informed consent.…”

Section: Methodsmentioning

confidence: 99%

Polymerase Chain Reaction for detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children from Cartagena, Colombia

2024

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Objectives: To detect the presence of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children from Cartagena and correlate it to dental caries precavity stages, applying a standardized PCR-based technique for epidemiological purposes. Methods: Descriptive study using a non-probabilistic sample of 50 children between 3 and 5 years of age, preschoolers from a Caribbean population in Colombia. Criteria for selection were that children should exhibit plaque accumulations on the surface of the cervical margins of the rearmost molars, and placed in one of two study groups: carious lesions and sound surfaces. Dental plaque samples from both groups were subjected to molecular analysis and statistical analysis was applied to determine the difference between the two groups using the frequencies of presence of S. mutans, S. sobrinus or both in the two groups applying Fisher’s exact test for association between the presence of microorganisms and the state of the tooth surface from where the dental plaque was taken. Results: The frequency of S. mutans in carious lesions was 76% and 24% in healthy surfaces. The frequency of S. sobrinus in carious lesions was 81.9% and 18.1% in caries-free surfaces. There was statistical significance between the presence of S. mutans and the presence of caries (p=0.001) and between the presence of S. sobrinus (p=0.02) and the presence of caries. There was no statistical significance between the presence of caries and the simultaneous presence of both microorganisms (p=0.08). Conclusions: The presence of S. mutans and S. sobrinus in dental plaque samples is highly prevalent and associated to non cavitated carious lesions, being the molecular identification of these microorganisms by PCR a sensitive, fast, and easy to use detection method for the mutans group of oral bacteria.

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Detección Molecular de Estreptococos Cariogénicos en Saliva

Cited by 12 publications

References 17 publications

Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus

Chemical and botanical characterization of Chilean propolis and biological activity on cariogenic bacteria Streptococcus mutans and Streptococcus sobrinus

Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

Polymerase Chain Reaction for detection of Streptococcus mutans and Streptococcus sobrinus in dental plaque of children from Cartagena, Colombia

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