Detección de genes de virulencia en cepas de Enterococcus faecalis susceptibles y resistentes a aminoglucósidos

Silva, Juan; Rodríguez, Y; Araya, Jorge E.; Gahona, Joselyne; Valenzuela, Nicomedes; Guerrero, Katheryne; Báez, John; Baquero, Fernando; Campo, Rosa del

doi:10.4067/s0716-10182013000100003

Cited by 2 publications

(4 citation statements)

References 18 publications

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“…The results obtained in our study show the presence of this gene in 61.2% of the samples from Uganda and 74.1% of those from Spain. These results are close to those obtained in similar studies [ 40 ]. We obtained a statistically significant relationship between increased biofilm development and the presence of this gene only in the Ugandan strains.…”

Section: Discussionsupporting

confidence: 93%

“…Ciprofloxacin resistance values are slightly higher in samples found in Uganda than in those from Spain, although they coincide with those obtained in other similar studies carried out between Chile and Spain [ 38 ]. Sensitivity to ampicillin in E. faecalis is 100% in both countries, in agreement with other recent studies [ 40 , 41 ].…”

Section: Discussionsupporting

confidence: 92%

“…The efaA gene, or E. faecalis antigen A, is usually characteristic of strains causing UTIs [ 40 ]. It is of great importance in the adhesion phases of the biofilm to collagen and cardiac or renal tissue [ 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

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Differences in Virulence Factors and Antimicrobial Susceptibility of Uropathogenic Enterococcus spp. Strains in a Rural Area of Uganda and a Spanish Secondary Hospital

et al. 2023

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Enterococcus faecalis and Enterococcus faecium have become two of the most important agents of nosocomial diseases due to their constantly growing resistance. Enterococcal infections are associated with biofilms, which are intrinsically sensitive to antimicrobials. The main goal of this study was to compare and relate their capacity to form biofilm and their antimicrobial sensitivity, as well as their virulence factors and their implicated genes, of strains isolated from patients with urinary tract infection (UTI) in a rural hospital in Uganda and a secondary hospital in Spain. A prospective study was conducted with 104 strains of E. faecalis and E. faecium isolated from patients with suspected UTI and who presented leukocyturia at the Saint Joseph Kitgum hospital (Uganda) and at the Hospital Universitario Principe de Asturias (Spain). All microorganisms were identified in Spain by MALDI-TOF mass spectrometry. Antimicrobial susceptibility studies were carried out using the Vitek® 2 system (Biomériux, France). The biofilm formation capacity was studied by photospectrometry. Phenotypic and genotypic virulence factors were studied in all cases by PCR or expression techniques. In Uganda, we found a higher incidence of E. faecium (65.3%, n = 32), contrary to the situation found in Spain where most of the bacteria found belonged to E. faecalis (92.7%, n = 51). All E. faecalis strains were found to have very low levels of resistance to ampicillin, imipenem, and nitrofurantoin. However, E. faecium exhibited more than 25% resistance to these antibiotics. Although the esp gene has been shown in the results obtained to be an important initial agent in biofilm formation, we have also demonstrated in this study the intervention of other genes when esp is not present, such as the ace1 gene. No statistically significant relationships were found between the presence of agg and gelE genes and increased biofilm formation. The significant difference between the incidence of E. faecalis and E. faecium and biofilm formation, between samples from Spain and Uganda, shows us very different profiles between countries.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 92%

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Differences in Virulence Factors and Antimicrobial Susceptibility of Uropathogenic Enterococcus spp. Strains in a Rural Area of Uganda and a Spanish Secondary Hospital

et al. 2023

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“…Like previous reports, our findings found that all HLAR E. faecalis isolates harbored efaA . 31 However, agg was not detected in any of the enterococci isolates studied herein. Similar to previous studies, 32 E. faecium and E. faecalis strains showed significantly different patterns with regard to the incidence of virulence determinants.…”

Section: Resultsmentioning

confidence: 63%

The prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in Inner Mongolia, China

Niu

et al. 2016

Brazilian Journal of Microbiology

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This study highlights the prevalence of aminoglycoside-modifying enzyme genes and virulence determinants among clinical enterococci with high-level aminoglycoside resistance in Inner Mongolia, China. Screening for high-level aminoglycoside resistance against 117 enterococcal clinical isolates was performed using the agar-screening method. Out of the 117 enterococcal isolates, 46 were selected for further detection and determination of the distribution of aminoglycoside-modifying enzyme-encoding genes and virulence determinants using polymerase chain reaction -based methods. Enterococcus faecium and Enterococcus faecalis were identified as the species of greatest clinical importance. The aac(6′)-Ie-aph(2″)-Ia and ant(6′)-Ia genes were found to be the most common aminoglycoside-modifying enzyme genes among high-level gentamicin resistance and high-level streptomycin resistance isolates, respectively. Moreover, gelE was the most common virulence gene among high-level aminoglycoside resistance isolates. Compared to Enterococcus faecium, Enterococcus faecalis harbored multiple virulence determinants. The results further indicated no correlation between aminoglycoside-modifying enzyme gene profiles and the distribution of virulence genes among the enterococcal isolates with high-level gentamicin resistance or high-level streptomycin resistance evaluated in our study.

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Detección de genes de virulencia en cepas de Enterococcus faecalis susceptibles y resistentes a aminoglucósidos

Cited by 2 publications

References 18 publications

Differences in Virulence Factors and Antimicrobial Susceptibility of Uropathogenic Enterococcus spp. Strains in a Rural Area of Uganda and a Spanish Secondary Hospital

Differences in Virulence Factors and Antimicrobial Susceptibility of Uropathogenic Enterococcus spp. Strains in a Rural Area of Uganda and a Spanish Secondary Hospital

The prevalence of aminoglycoside-modifying enzyme and virulence genes among enterococci with high-level aminoglycoside resistance in Inner Mongolia, China

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