Detecção de herpesvírus bovino 5 (BoHV-5) em bovinos do Sudeste Brasileiro

Gomes, Luciana I.; Rocha, Maurilio Andrade; Costa, Érica Azevedo; Lobato, Zélia Inês Portela; Mendes, L. C. N.; Borges, Alexandre S.; Leite, Rômulo Cerqueira; Barbosa-Stancioli, Edel Figueiredo

doi:10.1590/s0102-09352002000200014

Cited by 23 publications

(31 citation statements)

References 6 publications

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“…Similar studies with experimentally infected calves using DNA extracted from formalin-fixed, paraffin-embedded brain tissue detected 32.8% (Arruda et al 2010), 33.3% (Pedraza et al 2010) 66.6% (Gomes et al 2002), 75% (Ferrari et al 2007) and 87.5% (Ely et al 1996) of the positive animals using PCR, multiplex PCR, or nested PCR. Similar to our results, but using DNA extracted from frozen tissue, 100% of the BHV-5-positive animals using semi-nested PCR (Claus et al 2005) and PCR (Isernhagen et al 2011) were reported.…”

Section: Fig2 Adjusted Geometric Means* Of Lesions Distributed By Amentioning

confidence: 70%

Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves

et al. 2015

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RESUMO.-[Estudo histopatológico, imuno-histoquími-co e molecular da infecção por BHV-5 no sistema nervoso central de bovinos experimentalmente infectados.]A meningoencefalite bovina causada pelo BHV-5, um vírus DNA fita dupla envelopado que pertence à família Herpesviridae e subfamília Alphaherpesvirinae, é um importante diagnóstico diferencial das doenças do sistema nervoso central. O objetivo deste estudo foi descrever as alterações histológicas no sistema nervoso central de bovinos experimentalmente infectados com BHV-5 e comparar estas alterações com os resultados de imunoistoquímica (IHQ) e PCR. Amostras do sistema nervoso central de bezerros previamente inoculados com BHV-5 foram microscopicamente avaliadas e submetidas à IHQ e PCR. Todos os animais apresentaram meningoencefalite não-supurativa. Das 18 áreas avaliadas de cada bezerro, 32,41% e 35,13% foram positivas na IHQ e PCR, respectivamente. O telencéfalo apresentou lesões mais acentuadas e foi mais positivo na PCR do que as demais áreas encefálicas e se apresentou como a melhor área para coleta de material para o diagnóstico. As áreas positivas na IHQ e na PCR apresentaram lesões mais acentuadas do que as áreas negativas para as mesmas téc- Bovine meningoencephalitis caused by BHV-5, a double-stranded DNA enveloped virus that belongs to the family Herpesviridae and subfamily Alphaherpesvirinae, is an important differential diagnosis of central nervous diseases. The aim of this study was to describe the histological changes in the central nervous system of calves experimentally infected with BHV-5 and compare these changes with the PCR and IHC results. Formalin-fixed paraffin--embedded central nervous system samples from calves previously inoculated with BHV-5 were microscopically evaluated and tested using IHC and PCR. All the animals presented with nonsuppurative meningoencephalitis. From 18 evaluated areas of each calf, 32.41% and 35.19% were positive by IHC and PCR, respectively. The telencephalon presented more accentuated lesions and positive areas in the PCR than other encephalic areas and was the best sampling area for diagnostic purposes. Positive areas in the IHC and PCR were more injured than IHC and PCR negative areas. The animal with neurological signs showed more PCR-and IHC-positive areas than the other animals.INDEX TERMS: Bovine herpesvirus 5, diseases of cattle, meningoencephalitis, neuropathology.

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Section: Fig2 Adjusted Geometric Means* Of Lesions Distributed By Amentioning

confidence: 70%

Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves

et al. 2015

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“…As a consequence, the real seroprevalence of BoHV-1 and BoHV-5 infections is rather difficult to predict in regions where the two viruses co-circulate (d'Offay et al 1994, Vogel et al 2002. Nevertheless, in addition to the vast serological data, many clinic-pathological and virological reports have demonstrated the occurrence of both BoHV-1 and BoHV-5 infection and associated clinical diseases in our country (Riet-Correa et al 1989, Weiblen et al 1989, Flores et al 1998, Salvador et al 1998, Gomes et al 2002. In addition to the published reports, outbreaks of respiratory and/or genital disease and neurological disease with laboratory confirmation of the etiological agent (BoHV-1 and BoHV-5) have been frequently registered by regional diagnostic services in several states (Flores 2005, Silva et al 2010a, Silva et al 2010b, Oliveira et al 2011.…”

Section: Discussionmentioning

confidence: 98%

“…BoHV-5 has been identified in outbreaks of neurological disease in cattle in several Brazilian states, including Rio Grande do Sul (Riet-Correa et al 1989, Weiblen et al 1989, Roehe et al1998, Mato Grosso do Sul (Salvador et al 1998) and Minas Gerais (Gomes et al 2002). The seroprevalence of BoHV-5 infection among Brazilian cattle is difficult to ascertain with the routine serological tests due to the high percentage of crossreactivity with BoHV-1 what prevents the distinction between the antibody response to BoHV-1 and BoHV-5 (Bratanich et al 1991, Vogel et al 2002.…”

Section: Introductionmentioning

confidence: 99%

A Retrospective Search for Bovine Herpesvirus 5 (Bohv-5) in the Brain of Cattle Affected by Neurological Disease in Rio De Janeiro State

Pinto

Leite²,

Romijn³

et al. 2014

VR&R

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Bovine herpesvirus 5 (BoHV-5) is an important agent of meningoencephalitis in cattle and has been identified in outbreaks of neurological disease in several Brazilian states. However, no report of BoHV-5-associated neurological disease in Rio Janeiro state (RJ) has been published to date. This article reports a retrospective investigation for BoHV-5 performed in 146 brain specimens of cattle affected by neurological disease, submitted to the virology laboratory of the Empresa de Pesquisa Agropecuária do Estado do Rio de Janeiro (PESAGRO-RJ) between 1998 and 2001. Most specimens came from herds in the RJ state (130 or 89%) and a few from Minas Gerais counties (16 or 11%), close to the RJ border. All brain samples examined in this study were previously diagnosed negative for rabies virus. The specimens were submitted to PCR/nested-PCR for BoHV-5 sequences and virus isolation. From 146 analysed brains samples 48 (33%) were positive in at least one of the tests (PCR or virus isolation), 20 samples (14%) were positive in both assays, 19 samples (13%) were positive in Nested-PCR (n-PCR) and nine samples (6%) were characterized as BoHV-5 after virus isolation. The northeast region of the RJ contributed with most clinical specimens (92/146 or 63%) and presented the highest positivity (28/92 or 30%). These results demonstrated BoHV-5 association with neurological disease in bovine herds of RJ and MG and should therefore be included in differential diagnosis of neurological disease in cattle. The limitations of each of the diagnostic assays used here in led us to recommend the concomitant use of PCR and virus isolation to improve the sensitivity of the diagnosis of BoHV-5 infection.

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“…While BoHV-1 is the etiological agent of infectious bovine rhinotracheitis (IBR), and rarely causes neurological signs, BoHV-5 has been classically considered the causal agent of meningoencephalitis in young cattle of specific geographical areas, mainly Brazil, Argentina, the U.S. and Australia (Johnston et al, 1962;Carrillo et al, 1983;D'Offay et al, 1993;Salvador et al, 1998;Colodel et al, 2002). In Brazil, the occurrence of the disease has been reported in several geographic regions, with cases and/or outbreaks reported in the northern (RietCorrea et al, 2006), central western (Salvador et al, 1998;Colodel et al, 2002), southeastern (Salvador et al, 1998;Gomes et al, 2002), and southern regions (Claus et al, 2007;Rissi et al, 2008). The neurological signs observed in BoHV-5 infections are often multifocal deficits and comprise mainly cerebral and brainstem dysfunctions.…”

Section: Introductionmentioning

confidence: 99%

Neurological and epidemiological aspects of a BoHV-5 meningoencephalitis outbreak

Lunardi

Claus

Lisbôa

et al. 2009

Braz. arch. biol. technol.

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Detecção de herpesvírus bovino 5 (BoHV-5) em bovinos do Sudeste Brasileiro

Cited by 23 publications

References 6 publications

Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves

Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves

A Retrospective Search for Bovine Herpesvirus 5 (Bohv-5) in the Brain of Cattle Affected by Neurological Disease in Rio De Janeiro State

Neurological and epidemiological aspects of a BoHV-5 meningoencephalitis outbreak

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