Detailed studies on seed oil of Salicornia SOS-7 cultivated at the egyptian border of Red Sea

Abstract

Eight lipid patterns of Salicornia seed oil have been elucidated using HPLC as the main tool of analysis in conjunction with capillary GLC. The whole oil is rich in linoleic (66.5%) with lower amount of linolenic acid (1.4%). Of the 22 triglycerides (TG) determined, three major TG containing 3 and 2 linoleic acyls were detected by HPLC. The total tocopherols content (720 ppm) was determined directly in the oil by HPLC and it was found that alpha-T (49.1%) and gamma-T (48.2%) are predominating.<br />The whole sterol profile, as determined by GLC, includes cholesterol (1.0%), brassicasterol (1.4%), campesterol (2.4%), 5-stigmasterol (8.7%), beta-sitosterol (23.0%), spinasterol (17.0%), isofucosterol (0.8%), 7-stigmastenol (42.0%) and 7-avenasterol (3.7%). Furthermore, sterols patterns of the free (nonacylated) sterols (FS), acylated sterols (AS), free sterylglycosides (FSG) and acylated sterylglycosides (ASG) were determined by HPLC as their anthroylnitriles. FS and AS were determined as their steryl-9-anthroylnitriles (S-9-AN) whereas FSG and ASG were analysed as their sterylglycosidyl-1-anthroylnitriles (SG-1-AN) at nanogram level. <br /> Comparing Salicornia SOS-7 oil with traditional freshwater seed oils, Salicornia exhibits mostly a unique lipids constitution.<br><br>Se han dilucidado ocho perfiles lipídicos de aceite de semilla de Salicornia usando HPLC como principal herramienta de análisis junto con la CGL capilar. El aceite completo es rico en linoleico (66.5%) con bajo contenido en ácido linolénico (1.4%). De los 22 triglicéridos (TG) determinados, los tres mayoritarios que contenían 3 y 2 cadenas de linoleico se detectaron por HPLC. El contenido en tocoferoles totales (720 ppm) se determinó directamente en el aceite por HPLC y se encontró al alfa-T (49.1%) y al gamma-T (48.2%) como predominantes. El perfil de esteroles completo, determinado por CGL, incluyó colesterol (1.0%), brasicasterol (1.4%), campesterol (2.4%), 5-estigmasterol (8.7%), beta-sitosterol (23.0%), espinasterol (17.0%), isofucosterol (0.8%), 7-estigmastenol (42.0%) y 7-avenasterol (3.7%).<br /> Además, los perfiles de esteroles de los esteroles libres (sin acilar) (FS), esteroles adiados (AS), esterilglicósidos libres (FSG) y esterilglicósidos acilados (ASG) se determinaron por HPLC como sus antroilnitrilos. FS y AS se determinaron como sus esteril-9-antroilnitrilos (S-9-AN) mientras que FSG y ASG se analizaron como sus esterilglicosidil-1-antroilnitrilos (SG-1-AN) a niveles de nanogramos. <br /> Comparando el aceite de Salicornia SOS-7 con los aceites de semillas de agua dulce tradicionales, el de Salicornia mostró principalmente una constitución lipídica característica

Investigation of Liquid Wax Components of Egyptian Jojoba Seeds

“…HPLC 21,22) . Thus an oil sample (10mL of 10 % sample in hexane) was directly analyzed by HPLC according to the following conditions: column, YMC-A-012 silica, 6 ◊ 150 mm), isocratic elution, hexane/ isopropyl alcohol (100/0.5 by volume); flow rate, 2.0 mL/min; detector, fluorescence, excitation, 295 nm and emission at 325 nm.…”

Investigation of Lipids Profiles of Nigella, Lupin and Artichoke Seed Oils to be Used as Healthy Oils

“…The conditions were optimized to elute δ-T after 10 min; the results were automatically recorded as peak areas percentages by electronic integrator. From the peak area and the corresponding weight of each individual standard T, the weight of each individual T in the oil ppm can be calculated 17,22 .…”

“…The isolation of FS and AS, their derivatization into 9-AN and their HPLC determinations were carried out according to El-Mallah, et al 17,18 and Wanaka and Murui 20 . HPLC of sterols 9-AN was conducted under the following conditions: reversed phase column, ODS-1250Y, Senshu Pack 4.6 mm 250 mm ; detector, fl uorescence; excitation and emission wave lengths set at 360 and 460 nm; isocratic elution using acetonitrile/ dichloromethane 75/25, v/v at fl ow rate of 1mL/min.…”

“…The procedure of separation of FSG and ASG from the oil, their derivatization into SG-1-AN and HPLC analysis were carried out according to El-Mallah, et al 17,18 and…”

Chromatographic Quantitation of Some Bioactive Minor Components in Oils of Wheat Germ and Grape Seeds Produced as By-Products

“…The HPLC pattern of the standard tocopherol and tocotrienol designated as a-T, b-T, d-T, g-T, a-T3, b-T3, d-T3 and g-T3. From the peak area and the corresponding weight of each individual standard T and T3, the weight of each individual T and T3 in the oil (ppm) can be calculated 25,26) .…”