Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces

Sjollema, Jelmer; Mei, Henny C. van der; Hall, Connie L.; Peterson, Brandon W.; Vries, J. de; Song, Lei; Jong, Ed de; Busscher, Henk J.; Swartjes, Jan J. T. M.

doi:10.1038/s41598-017-04703-8

Cited by 38 publications

(50 citation statements)

References 47 publications

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“…This attachment is similar to the "weak rolling mode of surface adhesion" for E. coli 13 , yet the molecular origin may be different as the latter is known to bind to mannosylated surfaces via the adhesive protein FimH. The molecular mechanism we envision is akin to what has been proposed in a recent report 14 , albeit on different species of bacteria.…”

Section: The Proposed Mechanismsupporting

confidence: 55%

“…For instance, weak and reversible adhesion has been shown to enhance surface colonization 13 . Sequences of attachment and detachment of binding tethers may serve as pre-play in the process of consolidating irreversible attachment 14 . These transient attachments are also linked to optimal surface exploration as it has been proposed to maximize bacterial surface diffusivity 15 .…”

Section: Introductionmentioning

confidence: 99%

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Assessment of a weak mode of bacterial adhesion by applying an electric field

Tang

Araujo

Zheng

et al. 2020

Preprint

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Microbial attachment to surfaces is ubiquitous in nature. Most species of bacteria attach and adhere to surfaces via special appendages such as pili and fimbriae, the roles of which have been extensively studied. Here we report an experiment on pilus-less mutants of Caulobacter crescentus weakly attached to a plastic surface and subjected to an electric field parallel to the surface. We find that some individual cells transiently but repeatedly adhere to the surface in a stick-slip fashion in the presence of an electric field. Even while transiently detached, these bacteria move significantly slower than the unattached ones in the same field of view undergoing electrophoretic motion. We refer this behavior of repeated and transient attachment as "quasi-attachment". The speed of the quasi-attached bacteria exhibits large variations, frequently dropping close to zero for short intervals of time. This study suggests applying electric field as a useful method to investigate bacteria-surface interaction, which is significant in broader contexts such as infection and environmental control. SignificanceInteraction between bacteria and surfaces occur widely in nature, including those in industrial, environmental, and medical settings. It is therefore important to understand various mechanisms and factors that affect numerous forms of bacterium-surface interaction, particularly those resulting in adhesion or attachment, be they strong or weak, permanent or transient. This work takes a unique approach to identify a transient and reversible mode of bacterial attachment to a solid surface, by applying an electric field to exert a force for detachment. The force thus exerted proves to reach the amplitude required to detach bacteria of a pilus-less strain that weakly attach to a plastic surface. The method may be applied broadly to investigate bacteria-surface interaction.

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Section: The Proposed Mechanismsupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Assessment of a weak mode of bacterial adhesion by applying an electric field

Tang

Araujo

Zheng

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…For adhesion force measurements, single bacterial contact probes 47 were prepared as described before 7,48 . First, S. mutans was attached to a tipless cantilever (NP-O10; Bruker AFM Probes, Camarillo, CA, USA) via electrostatic double-layer interaction with poly-L-lysine (PLL) (molecular weight 70,000 to 150,000; Sigma-Aldrich, St. Louis, MO, USA) adsorbed to the cantilever using a micromanipulator (Narishige Groups, Tokyo, Japan).…”

Section: Adhesion Force Measurementsmentioning

confidence: 99%

Streptococcus mutans adhesion force sensing in multi-species oral biofilms

Wang

Mei

Busscher

et al. 2020

npj Biofilms Microbiomes

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Bacteria utilize chemical and mechanical mechanisms to sense their environment, to survive hostile conditions. In mechanical sensing, intra-bilayer pressure profiles change due to deformation induced by the adhesion forces bacteria experience on a surface. Emergent properties in mono-species Streptococcus mutans biofilms, such as extracellular matrix production, depend on the adhesion forces that streptococci sense. Here we determined whether and how salivary-conditioning film (SCF) adsorption and the multi-species nature of oral biofilm influence adhesion force sensing and associated gene expression by S. mutans. Hereto, Streptococcus oralis, Actinomyces naeslundii, and S. mutans were grown together on different surfaces in the absence and presence of an adsorbed SCF. Atomic force microscopy and RT-qPCR were used to measure S. mutans adhesion forces and gene expressions. Upon SCF adsorption, stationary adhesion forces decreased on a hydrophobic and increased on a hydrophilic surface to around 8 nN. Optical coherence tomography showed that triple-species biofilms on SCF-coated surfaces with dead S. oralis adhered weakly and often detached as a contiguous sheet. Concurrently, S. mutans displayed no differential adhesion force sensing on SCF-coated surfaces in the triple-species biofilms with dead S. oralis, but once live S. oralis were present S. mutans adhesion force sensing and gene expression ranked similar as on surfaces in the absence of an adsorbed SCF. Concluding, live S. oralis may enzymatically degrade SCF components to facilitate direct contact of biofilm inhabitants with surfaces and allow S. mutans adhesion force sensing of underlying surfaces to define its appropriate adaptive response. This represents a new function of initial colonizers in multispecies oral biofilms.

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“…Indeed, this is almost always applicable to viruses since the wet and dry depositions of viruses are usually associated with either bacteria or particulate matter (PM) [367,368]. However, the earlier the first interfacial adhesion is initiated, the sooner the effect of other forces take place [369].…”

Section: Self-cleaningmentioning

confidence: 99%

Blood Pressure Sensors: Materials, Fabrication Methods, Performance Evaluations and Future Perspectives

Al-Qatatsheh

Morsi

Zavabeti

et al. 2020

Sensors

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Advancements in materials science and fabrication techniques have contributed to the significant growing attention to a wide variety of sensors for digital healthcare. While the progress in this area is tremendously impressive, few wearable sensors with the capability of real-time blood pressure monitoring are approved for clinical use. One of the key obstacles in the further development of wearable sensors for medical applications is the lack of comprehensive technical evaluation of sensor materials against the expected clinical performance. Here, we present an extensive review and critical analysis of various materials applied in the design and fabrication of wearable sensors. In our unique transdisciplinary approach, we studied the fundamentals of blood pressure and examined its measuring modalities while focusing on their clinical use and sensing principles to identify material functionalities. Then, we carefully reviewed various categories of functional materials utilized in sensor building blocks allowing for comparative analysis of the performance of a wide range of materials throughout the sensor operational-life cycle. Not only this provides essential data to enhance the materials’ properties and optimize their performance, but also, it highlights new perspectives and provides suggestions to develop the next generation pressure sensors for clinical use.

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Detachment and successive re-attachment of multiple, reversibly-binding tethers result in irreversible bacterial adhesion to surfaces

Cited by 38 publications

References 47 publications

Assessment of a weak mode of bacterial adhesion by applying an electric field

Assessment of a weak mode of bacterial adhesion by applying an electric field

Streptococcus mutans adhesion force sensing in multi-species oral biofilms

Blood Pressure Sensors: Materials, Fabrication Methods, Performance Evaluations and Future Perspectives

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