Detached Leaf Assay to Screen for Host Plant Resistance to &lt;I&gt;Helicoverpa armigera&lt;/I&gt;

Sharma, H. C.; Pampapathy, G.; Dhillon, Mukesh K.; Ridsdill-Smith, T. J.

doi:10.1603/0022-0493-98.2.568

Cited by 20 publications

(2 citation statements)

References 6 publications

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“…Polyphagous insect pest, H. armigera (Hübner) (Lepidoptera: Noctuidae), is distributed worldwide and lead to average crop loss of approximately 2 billion USD annually (Jiang et al, 2015; Sharma et al, 2005; Xu et al, 2012). Due to remarkable chemosensory adaptation, these insects have developed enormous dietary flexibility (Agnihotri et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Diminution of feeding response in Helicoverpa armigera by inhibition or silencing of sugar gustatory receptor

Nagare

et al. 2019

Preprint

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Gustatory receptor (GR) is one of the essential chemosensory molecules in Lepidopteran pests. GR is involved in sensing several canonical tastes which in turn regulate the diverse behavioral and physiological responses of these insects. In this article, we have evaluated the alteration in feeding response of Helicoverpa armigera by blocking and silencing of sugar-sensing gustatory receptor 9 (HarmGr9). Sf9 cells based assay showed that glucose analogue, Miglitol, can bind to the expressed HarmGr9. This binding might lead to an inhibition of receptor activation and downstream signaling, indicated by reduced intracellular Ca2+ fluorescence. Further, the in-vivo study illustrated the feeding rate reduced on a diet containing miglitol as compared to the larvae fed on the artificial diet. Reduction in feeding rate was prolonged in insects fed on the miglitol containing diet even after switching to the control glucose diet. Competitive cell-based assay and feeding assay, using equimolar glucose and miglitol, also showed an inhibitory effect on HarmGr-9 activation and insect feeding rate. We have observed similar feeding rate reduction in HarmGr9 knockdown in H. armigera larvae. We believe this unique approach of H. armigera feeding response inhibition by blocking the sugar receptor can be further used to develop a novel strategy for agricultural pest management.

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Section: Introductionmentioning

confidence: 99%

Diminution of feeding response in Helicoverpa armigera by inhibition or silencing of sugar gustatory receptor

Nagare

et al. 2019

Preprint

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“…Varieties with moderate levels of resistance to pod borers have also been developed, and/or released for cultivation to the farmers (Sharma et al 2005, 2010). Genotypes such as ICPL 187-1, ICP 7203-1, ICPL 98008, T 21, ICP 7035 and ICPL 332 have shown moderate levels of resistance to H. armigera across planting dates (Kumari et al 2010a).…”

Section: Introductionmentioning

confidence: 99%

Pigeonpea genotypes influence parasitization preference and survival and development of the Helicoverpa armigera larval parasitoid, Campoletis chlorideae

2014

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Studies were undertaken to identify pigeonpea, Cajanus cajan (L.) Millspaugh and the wild relative of pigeonpea, Cajanus scarabaeoides (L.) (accession ICPW 125,) genotypes that are hospitable to the pod borer, Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) larval parasitoid, Campoletis chlorideae Uchida (Hymenoptera: Ichneumonidae) for the management of this pest in pigeonpea based cropping systems. Percentage parasitization of the H. armigera larvae by the C. chlorideae females was greater under no-choice conditions than under multi-choice conditions because of forced parasitization under no-choice conditions. Lowest parasitization was recorded on the wild relative, ICPW 125, which may be due to long nonglandular hairs and low survival of H. armigera larvae. Parasitization of H. armigera larvae was greater under no-choice, dual-choice and/or multi-choice conditions on ICPL 87, ICPL 87119 and ICPL 87091, which are susceptible to H. armigera, than on the pod borer-resistant genotypes ICPL 332WR, ICPL 84060 and ICPB 2042; while survival and development of the parasitoid was better on H. armigera larvae fed on ICPL 87, ICPL 87119, LRG 41, ICP 7035 and ICPL 87091 than on ICPL 332WR, ICPL 84060, ICPB 2042 and ICPW 125. The genotypes ICPL 87, ICPL 87119, LRG 42 and ICPL 87091 that are hospitable to C. chloridae, are better suited for use in integrated pest management to minimize the losses due to H. armigera in pigeonpea.

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Jasmonic Acid-Mediated-Induced Resistance in Groundnut (Arachis hypogaea L.) Against Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)

War

Paulraj

War³

et al. 2011

J Plant Growth Regul

101

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Detached Leaf Assay to Screen for Host Plant Resistance to <I>Helicoverpa armigera</I>

Cited by 20 publications

References 6 publications

Diminution of feeding response in Helicoverpa armigera by inhibition or silencing of sugar gustatory receptor

Diminution of feeding response in Helicoverpa armigera by inhibition or silencing of sugar gustatory receptor

Pigeonpea genotypes influence parasitization preference and survival and development of the Helicoverpa armigera larval parasitoid, Campoletis chlorideae

Jasmonic Acid-Mediated-Induced Resistance in Groundnut (Arachis hypogaea L.) Against Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae)

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