Desulfovibrio legallis sp. nov.: A Moderately Halophilic, Sulfate-Reducing Bacterium Isolated from a Wastewater Digestor in Tunisia

Thabet, Olfa Ben Dhia; Wafa, Terres; Khelifi, Eltaief; Cayol, Jean‐Luc; Hamdi, Moktar; Fauque, Guy; Fardeau, Marie‐Laure

doi:10.1007/s00284-010-9733-z

Cited by 33 publications

(10 citation statements)

References 25 publications

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“…65,66 The most abundant Desulfovibrio OTU was identified to resemble Desulfovibrio legallii H1 (95% coverage and 99% similarity, SI Table S20), which is also known to be able to perform direct ethanol oxidation. 66 These type of sulfate reducing bacteria are versatile in bioelectrochemical processes and can donate or take up electrons from electrodes while converting various organics or CO 2 . Possibly the Desulfovibrionales were (partly) responsible for the usage of electrons or H 2 while they were reducing CO 2 to acetate and/or forming alcohols.…”

Section: Resultsmentioning

confidence: 99%

Branched Medium Chain Fatty Acids: Iso-Caproate Formation from Iso-Butyrate Broadens the Product Spectrum for Microbial Chain Elongation

Leeuw

Buisman

Strik

2019

Environ. Sci. Technol.

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Chain elongation fermentation can be used to convert organic residues into biobased chemicals. This research aimed to develop a bioprocess for branched medium chain fatty acids (MCFAs) production. A long-term continuous reactor experiment showed that iso-caproate (4-methyl pentanoate, i-C6) can be produced via ethanol based chain elongation. The enriched microbiome formed iso-caproate from iso-butyrate at a rate of 44 ± 6 mmol C L–1 day–1 during the last phase. This amounted to 20% of all formed compounds based on carbon atoms. The main fermentation product was n-caproate (55% of all carbon), as a result of acetate and subsequent n-butyrate elongation. The microbiome preferred straight-chain elongation over branched-chain elongation. Lowering the acetate concentration in the influent led to an increase of excessive ethanol oxidation (EEO) into electron equivalents (e.g., H2) and acetate. The formed acetate in turn stimulated straight chain elongation, but the resulting lower net acetate supply rate towards straight chain elongation led to an increased selectivity towards and productivity of i-C6. The electrons produced via oxidation routes and chain elongation were apparently utilized by hydrogenotrophic methanogens, homoacetogens, and carboxylate-to-alcohol reducing bacteria. Further improvements could be achieved if the acetate-producing EEO was minimized and limitations of ethanol and CO2 were prevented.

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Section: Resultsmentioning

confidence: 99%

Branched Medium Chain Fatty Acids: Iso-Caproate Formation from Iso-Butyrate Broadens the Product Spectrum for Microbial Chain Elongation

Leeuw

Buisman

Strik

2019

Environ. Sci. Technol.

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“…Our report expands on the species which may cause human infections, including D. legallii, which is a novel species first described in a Tunisian wastewater digester in 2011 (1), and also two cases of D. intestinalis bacteremia. Interestingly, D. intestinalis, which was first described in termite hindguts (23), was recently described as part of vaginal flora in 3% of women in a Japanese study (11).…”

Section: Desulfuricans)mentioning

confidence: 99%

“…PrepMan Ultra (Life Technologies, Grand Island, NY) was used to prepare DNA for partial 16S rRNA gene PCR using primers 5=-T GGAGAGTTTGATCCTGGCTCAG-3= and 5=-TACCGCGGCT GCTGGCAC-3= with bidirectional amplification product sequencing performed using the same primers. A 496-bp sequence was generated, revealing 100% identity to Desulfovibrio legallii (strain H 1 ) (base pairs 4 to 499 of GenBank accession number FJ225426) (1,2). Per our laboratory protocol, for species identification, a Ն99% agreement with a database strain with Ͼ0.8% separation from other species is required.…”

mentioning

confidence: 99%

Desulfovibrio legallii Prosthetic Shoulder Joint Infection and Review of Antimicrobial Susceptibility and Clinical Characteristics of Desulfovibrio Infections

et al. 2014

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We describe a case of shoulder hemiarthroplasty infection with Desulfovibrio legallii. Antimicrobial susceptibilities of 36 Desulfovibrio isolates are presented. Metronidazole and carbapenems exhibited reliable activity, although piperacillin-tazobactam did not. Eleven previous cases of Desulfovibrio infection are reviewed; most arose from a gastrointestinal tract-related source. CASE REPORT In July 2013, a 70-year-old woman with a 10-year history of palmoplantar psoriasis presented to our institution with a 6-month history of intermittent fever, chills, and left-shoulder pain. This had worsened over the preceding 2 weeks, and she had developed a diminished range of movement of her left shoulder. She had sustained a closed proximal humerus fracture which had been treated with a humeral endoprosthesis implanted 18 years prior to presentation. For about 8 years through August 2012, she had received treatment for psoriasis with twice-yearly intramuscular injections of 80 mg triamcinolone. In August 2012, she was placed on topical treatment and phototherapy. In attempt to improve her psoriasis, the patient reported soaking her hands and feet, which had many fissures and cracks, in Lake Superior and in the Pacific Ocean. Physical examination revealed a well-healed surgical scar over her left shoulder and a scant amount of swelling in the lower axilla. She had an elevated white cell count of 16 ϫ 10 9 /liter, an erythrocyte sedimentation rate of 35 mm/h (normal, 0 to 29 mm/h), and a C-reactive protein level of 204.3 mg/liter (normal, Յ8 mg/liter). An indium 111 bone scan showed asymmetric uptake around her left shoulder suggestive of periprosthetic infection. The cell count in the aspirated shoulder joint fluid was 25,075 white blood cells/l, with 99% neutrophils. Cultures of the aspirated joint fluid yielded moderate growth of small, pinpoint, clear colonies on CDC anaerobic sheep blood agar after 6 days of anaerobic incubation at 35°C (Fig. 1A); broth cultures were negative. The colonies measured about 1 mm in diameter and were graygreen and convex after 10 days of incubation (Fig. 1B). Gram stain showed spiral-shaped Gram-negative bacilli (Fig. 1C) which were motile, indole negative, and catalase positive with 15% H 2 O 2 . PrepMan Ultra (Life Technologies, Grand Island, NY) was used to prepare DNA for partial 16S rRNA gene PCR using primers 5=-T GGAGAGTTTGATCCTGGCTCAG-3= and 5=-TACCGCGGCT GCTGGCAC-3= with bidirectional amplification product sequencing performed using the same primers. A 496-bp sequence was generated, revealing 100% identity to Desulfovibrio legallii (strain H 1 ) (base pairs 4 to 499 of GenBank accession number FJ225426) (1, 2). Per our laboratory protocol, for species identification, a Ն99% agreement with a database strain with Ͼ0.8% separation from other species is required. The next-best matches were Desulfovibrio sp. canine oral taxon 070 clone 1P043 (GenBank accession number JQ295257) (96% match) and Desulfovibrio sp. canine oral taxon 070 clone 1J008 (GenBank accession number JQ294906) (95% ...

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“…The observed Desufovibrio most likely work in conjunction with the Anaerolineaceaea ICG-001 and Methanoculleus palmolei. Desulfovibrio are known to be able to perform βoxidation in the absence of an extracellular electron acceptor 222,223 . The most abundant Desulfovibrio OTU was identified to resemble Desulfovibrio legallii H1 (95% coverage and 99% similarity, Table S20), which is also known to be able to perform direct ethanol oxidation 223 .…”

Section: Microbial Community Analysismentioning

confidence: 99%

“…Desulfovibrio are known to be able to perform βoxidation in the absence of an extracellular electron acceptor 222,223 . The most abundant Desulfovibrio OTU was identified to resemble Desulfovibrio legallii H1 (95% coverage and 99% similarity, Table S20), which is also known to be able to perform direct ethanol oxidation 223 . These type of sulfate reducing bacteria are versatile in bioelectrochemical processes and can donate or take up electrons from electrodes while converting various organics or CO2.…”

Section: Microbial Community Analysismentioning

confidence: 99%

Open culture chain elongation for branched carboxylate formation

Leeuw¹,

Daniël²

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How to create an environment that selects for decision making to favor the development of a circular economy, however, is a very complex endeavor and fortunately does not fall any further into the scope of this thesis. Inside the scope of this thesis is a much simpler research on how to use mixed culture biotechnology principles to enrich microbiomes that can be used to recycle organic residue streams via production of useful biochemicals. elongated with an acetyl-CoA unit in a Claisen condensation reaction that is catalyzed by a thiolase 34 . The acetyl-CoA elongation unit is usually derived from oxidation of the electron donor, whereas electrons from this oxidation are used to reduce the elongation product after condensation. It results in an elongated carboxylate as metabolic end-product that has been extended by two hydrated carbon atoms. However, limiting the bicarbonate availability too much will also affect the growth of ethanol based chain elongator: Clostridium kluyveri, because it requires bicarbonate for biomass formation 53 . For methanol based chain elongation bicarbonate can be a direct substrate for the formation of acetate and butyrate 43 . Though, recent research has also shown that methanol based chain elongation of acetate, without the utilization of bicarbonate, is possible 45 . Bicarbonate dosing in this case should be dependent on the desired methanol based chain elongation conversions. Another important selection pressure tool is the possibility to enforce a certain minimum growth rate by setting the hydraulic retention time of a reactor during continuous operation 15 Acetoclastic methanogenesis 69 CH3COO -+ H2O  CH4 + HCO3 --31.05 -31.05 -31.05 ΔG 0' biological standard conditions (pH 7) ΔG 1 hydrogen partial pressure = 1 kPa ΔG 2 hydrogen partial pressure = 1 Pa *

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Desulfovibrio legallis sp. nov.: A Moderately Halophilic, Sulfate-Reducing Bacterium Isolated from a Wastewater Digestor in Tunisia

Cited by 33 publications

References 25 publications

Branched Medium Chain Fatty Acids: Iso-Caproate Formation from Iso-Butyrate Broadens the Product Spectrum for Microbial Chain Elongation

Branched Medium Chain Fatty Acids: Iso-Caproate Formation from Iso-Butyrate Broadens the Product Spectrum for Microbial Chain Elongation

Desulfovibrio legallii Prosthetic Shoulder Joint Infection and Review of Antimicrobial Susceptibility and Clinical Characteristics of Desulfovibrio Infections

Open culture chain elongation for branched carboxylate formation

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