Desulfotomaculum alkaliphilum sp. nov., a new alkaliphilic, moderately thermophilic, sulfate-reducing bacterium.

Pikuta, Elena V.; Lysenko, A; Сузина, Н. Е.; Осипов, Г. А.; Кузнецов, Б. Б.; Tourova, T. P.; Akimenko, V. K.; Laurinavichius, Kestutis S.

doi:10.1099/00207713-50-1-25

Cited by 59 publications

(30 citation statements)

References 28 publications

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“…Isolated from mud sediments of alkaline, hypersaline, soda Mono Lake in California, North America. Zhilina et al (1997)]; 4, Desulfotomaculum alkaliphilum S1 T [data from Pikuta et al (2000)]. +, Positive; 2, negative; (+), slow growth; ND, not determined.…”

Section: Discussionmentioning

confidence: 99%

“…All experiments were performed at 35 u C. Obligate dependence on the CO 3 22 ion was detected by using a medium that was described previously (Pikuta et al, 2000). Dependence on Na + ions was detected by substitution of Na-containing salts by K-containing salts.…”

Section: Growth Characteristics and Metabolic Propertiesmentioning

confidence: 99%

“…Both genera include Gram-negative, non-spore-forming, mesophilic, obligately carbonate-dependent alkaliphiles that are incapable of growth below pH 8?0. The moderately thermophilic and alkaliphilic, spore-forming sulfate-reducer Desulfotomaculum alkaliphilum was isolated from manure with neutral pH (Pikuta et al, 2000), indicating that the adaptation of microorganisms to alkalic environments is not connected with deep genetic changes and that local micro-ecosystems with high pH and temperature could be created in composite substrates, such as soil or manure, by these micro-organisms. All previously described alkaliphilic sulfate-reducers had a lithoheterotrophic metabolism.…”

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Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth

Pikuta

Hoover

Bej

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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A novel alkaliphilic, sulfate-reducing bacterium, strain MLF1 T , was isolated from sediments of soda Mono Lake, California. Gram-negative vibrio-shaped cells were observed, which were 0?6-0?761?2-2?7 mm in size, motile by a single polar flagellum and occurred singly, in pairs or as short spirilla. Growth was observed at 15-48 6C (optimum, 37 6C), >1-7 % NaCl, w/v (optimum, 3 %) and pH 8?0-10?0 (optimum, 9?5). The novel isolate is strictly alkaliphilic, requires a high concentration of carbonate in the growth medium and is obligately anaerobic and catalasenegative. As electron donors, strain MLF1 T uses hydrogen, formate and ethanol. Sulfate, sulfite and thiosulfate (but not sulfur or nitrate) can be used as electron acceptors. The novel isolate is a lithoheterotroph and a facultative lithoautotroph that is able to grow on hydrogen without an organic source of carbon. Strain MLF1 T is resistant to kanamycin and gentamicin, but sensitive to chloramphenicol and tetracycline. The DNA G+C content is 63?0 mol% (HPLC). DNA-DNA hybridization with the most closely related species, Desulfonatronum lacustre Z-7951 T , exhibited 51 % homology. Also, the genome size (1?6610 9 Da) and T m value of the genomic DNA (71±2 6C) for strain MLF1 T were significantly different from the genome size (2?1610 9 Da) and T m value (63±2 6C) for Desulfonatronum lacustre Z-7951 T . On the basis of physiological and molecular properties, the isolate was considered to be a novel species of the genus Desulfonatronum, for which the name Desulfonatronum thiodismutans sp. nov. is proposed (the type strain is MLF1 T

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Section: Discussionmentioning

confidence: 99%

Section: Growth Characteristics and Metabolic Propertiesmentioning

confidence: 99%

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confidence: 99%

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Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth

Pikuta

Hoover

Bej

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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“…The previously described mesophilic (optimal temperature 25-40 u C) Desulfotomaculum species are D. ruminis (Campbell & Postgate, 1965), D. acetoxidans (Widdel & Pfennig, 1977), D. sapomandens (Cord-Ruwisch & Garcia, 1985), D. aeronauticum (Hagenauer et al, 1997), D. halophilum (Tardy-Jacquenod et al, 1998) and D. gibsoniae (Kuever et al, 1999). The thermophilic (optimal temperature 50-70 u C) species are D. alkaliphilum (Pikuta et al, 2000), D. australicum (Love et al, 1993), D. carboxydivorans (Parshina et al, 2005), D. geothermicum (Daumas et al, 1988), D. kuznetsovii (Nazina et al, 1989), D. luciae (Liu et al, 1997), D. nigrificans (Werkman & Weaver, 1927;Campbell & Postgate, 1965), D. putei (Liu et al, 1997), D. solfataricum (Goorissen et al, 2003), D. thermoacetoxidans (Min & Zinder, 1990), D. thermobenzoicum with two subspecies, namely subsp. thermobenzoicum and subsp.…”

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confidence: 99%

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Kaksonen

Spring

Schümann

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal-and sulfate-containing wastewater A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1 T , was rod-shaped and motile. The temperature range for growth was 33-51 6C (optimum 44-46 6C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C 15 : 0 , iso-C 17 : 1 v10c and iso-C 17 : 0 . Strain RE35E1 T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038 T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475 T (92.0 % similarity). Strain RE35E1 T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1 T (5DSM 16058 T 5JCM 14019 T ).The genus Desulfotomaculum constitutes a group of rodshaped, endospore-forming, sulfate-reducing bacteria. The genus currently includes mesophilic, moderately thermophilic and thermophilic species (Werkman & Weaver, 1927;Campbell & Postgate, 1965;Vandieken et al., 2006). et al., 1996), D. thermosapovorans (Fardeau et al., 1995) and D. thermosubterraneum (Kaksonen et al., 2006b). Additionally, the reclassification of 'Desulfotomaculum nigrificans subsp. salinus' as a novel species ('Desulfotomaculum salinum') has been proposed (Nazina et al., 2005). Currently, Desulfotomaculum arcticum is the only sulfate-reducing member of the genus that has an optimal temperature above 40 uC and below 50 uC (Vandieken et al., 2006). Recently, we reported the isolation of sulfate-reducers from fluidized-bed reactors (FBRs) treating acidic metal-and sulfate-containing wastewater (Kaksonen et al., 2004c). This paper describes one of those isolates, strain RE35E1 T , a novel, moderately thermophilic, spore-forming, sulfatereducing bacterium.Abbreviation: FBR, fluidized-bed reactor.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain RE35E1 T is AY548778.

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“…The genus Desulfotomaculum was first described by Campbell & Postgate (1965). At the time of writing, the genus comprises 15 thermophilic species: D. alkaliphilum (Pikuta et al, 2000), D. arcticum (Vandieken et al, 2006), D. australicum (Love et al, 1993), D. carboxydivorans (Parshina et al, 2005), D. geothermicum (Daumas et al, 1988), D. kuznetsovii (Nazina et al, 1989), D. luciae (Liu et al, 1997), D. nigrificans (Werkman & Weaver, 1927;Campbell & Postgate, 1965), D. putei (Liu et al, 1997), 'D. salinum' (Nazina et al, 2005), D. solfataricum (Goorissen et al, 2003), D. thermoacetoxidans (Min & Zinder, 1990), D. thermobenzoicum with two subspecies, namely subsp.…”

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Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area

Kaksonen

Spring

Schümann

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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A thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from an underground mine in a geothermally active area in Japan. Cells of this strain, designated RL50JIIIT, were rod-shaped and motile. The temperature range for growth was 50–72 °C (optimum growth at 61–66 °C) and the pH range was 6.4–7.8 (optimum at pH 7.2–7.4). Strain RL50JIIIT tolerated up to 1.5 % NaCl, but optimum growth occurred in the presence of 0–1 % NaCl. Electron acceptors utilized were sulfate, sulfite, thiosulfate and elemental sulfur. Electron donors utilized were H2 in the presence of CO2, alanine, various carboxylic acids and alcohols. Fermentative growth occurred on lactate and pyruvate. The cell wall contained mesodiaminopimelic acid and the major respiratory isoprenoid quinone was menaquinone 7 (MK-7). Major whole-cell fatty acids were iso-C15 : 0, iso-C17 : 0 DMA (dimethyl acetal), iso-C15 : 0 DMA and iso-C17 : 0. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed 98.7 % similarity with Desulfotomaculum solfataricum DSM 14956T. However, DNA–DNA hybridization experiments with Desulfotomaculum kuznetsovii, Desulfotomaculum luciae and D. solfataricum and the G+C content of the DNA (54.4 mol%) allowed the differentiation of strain RL50JIIIT from the recognized species of the genus Desulfotomaculum. Strain RL50JIIIT therefore represents a novel species, for which the name Desulfotomaculum thermosubterraneum sp. nov. is proposed. The type strain is RL50JIIIT (=DSM 16057T=JCM 13837T).

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Desulfotomaculum alkaliphilum sp. nov., a new alkaliphilic, moderately thermophilic, sulfate-reducing bacterium.

Cited by 59 publications

References 28 publications

Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth

Desulfonatronum thiodismutans sp. nov., a novel alkaliphilic, sulfate-reducing bacterium capable of lithoautotrophic growth

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Desulfotomaculum thermosubterraneum sp. nov., a thermophilic sulfate-reducer isolated from an underground mine located in a geothermally active area

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