Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal- and sulfate-containing wastewater

Abstract

Desulfotomaculum alcoholivorax sp. nov., a moderately thermophilic, spore-forming, sulfate-reducer isolated from a fluidized-bed reactor treating acidic metal-and sulfate-containing wastewater A moderately thermophilic, Gram-positive, endospore-forming, sulfate-reducing bacterium was isolated from a fluidized-bed reactor treating acidic water containing metal and sulfate. The strain, designated RE35E1 T , was rod-shaped and motile. The temperature range for growth was 33-51 6C (optimum 44-46 6C) and the pH range was 6.0-7.5 (optimum pH 6.4-7.3). The strain grew optimally without additional NaCl. The electron acceptors were 10 mM sulfate, thiosulfate and elemental sulfur and 1 mM (but not 10 mM) sulfite. Various alcohols and carboxylic acids were utilized as electron donors. Fermentative growth occurred on pyruvate. The cell wall contained meso-diaminopimelic acid, and the major respiratory isoprenoid quinone was menaquinone MK-7. The major whole-cell fatty acids were iso-C 15 : 0 , iso-C 17 : 1 v10c and iso-C 17 : 0 . Strain RE35E1 T was related to representatives of the genera Desulfotomaculum and Sporotomaculum, the closest relatives being Desulfotomaculum arcticum DSM 17038 T (96.3 % 16S rRNA gene sequence similarity) and Sporotomaculum hydroxybenzoicum DSM 5475 T (92.0 % similarity). Strain RE35E1 T represents a novel species, for which the name Desulfotomaculum alcoholivorax sp. nov. is proposed. The type strain is RE35E1 T (5DSM 16058 T 5JCM 14019 T ).The genus Desulfotomaculum constitutes a group of rodshaped, endospore-forming, sulfate-reducing bacteria. The genus currently includes mesophilic, moderately thermophilic and thermophilic species (Werkman & Weaver, 1927;Campbell & Postgate, 1965;Vandieken et al., 2006). et al., 1996), D. thermosapovorans (Fardeau et al., 1995) and D. thermosubterraneum (Kaksonen et al., 2006b). Additionally, the reclassification of 'Desulfotomaculum nigrificans subsp. salinus' as a novel species ('Desulfotomaculum salinum') has been proposed (Nazina et al., 2005). Currently, Desulfotomaculum arcticum is the only sulfate-reducing member of the genus that has an optimal temperature above 40 uC and below 50 uC (Vandieken et al., 2006). Recently, we reported the isolation of sulfate-reducers from fluidized-bed reactors (FBRs) treating acidic metal-and sulfate-containing wastewater (Kaksonen et al., 2004c). This paper describes one of those isolates, strain RE35E1 T , a novel, moderately thermophilic, spore-forming, sulfatereducing bacterium.Abbreviation: FBR, fluidized-bed reactor.The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain RE35E1 T is AY548778.

Probing the diversity of chloromethane-degrading bacteria by comparative genomics and isotopic fractionation

“…Strikingly and in contrast to all strains growing with CH 3 Cl using the cmu pathway described so far, all these strains are anaerobes. Three of them are Gram-negative bacteria from the class Deltaproteobacteria, i.e., Desulfotomaculum alcoholivorax ( Kaksonen et al, 2008 ), Desulfurispora thermophila ( Kaksonen et al, 2007 ) and Desulfomonile tiedjei ( DeWeerd et al, 1990 ), and two belong to the class Clostridia, i.e., the Gram-positive Thermincola potens ( Byrne-Bailey et al, 2010 ) and the Gram-negative Thermosediminibacter oceani ( Pitluck et al, 2010 ). Notably, Desulfomonile tiedjei has a second cmu cluster containing only cmuB and cmuA ( Figure 1 ) 6 kb away from a cmuBCA gene cluster.…”

Quorum Sensing and the Use of Quorum Quenchers as Natural Biocides to Inhibit Sulfate-Reducing Bacteria

“…is relatively better studied than other types of SRB, many SRBs that are present in seawater cooling tower systems or in the oil field injection systems are not Desulfovibrio spp., but instead belong to the thermophilic gram-positive Desulfotomaculum spp. [77] or other gram-negative bacteria like Desulfonauticus spp. [78].…”

Role of Several Types of Inhibitors Used In Metal Corrosion for Mitigation and Their Impacts : A Brief Overview

“…[15] orientis Curved rod aeronauticum Rod [16] Desulfotomaculum alcoholivorax Rod Gram positive bacteria [17] alkaliphilum Curved rod [18] aquiferis Rod [19] arcticum Rod Gram positive bacteria [20] auripigmentum Sausage-shaped Gram positive bacteria [21] carboxydivorans Spore-forming rods Gram positive bacteria [22] copahuensis Curved rod Gram positive bacteria [23] Desulfomicrobium salsuginis Rod Gram negative bacteria [24] aestuarii baculatum [25] Desulfobulbus propionicus Lemon shaped/Ovoid/Ellip soidal Gram negative bacteria [26] aggregans Ovoid [27] alkaliphilus Lemon-shaped oval rods [28] elongatus Motile rods/slightly curved [29] japonicus Non-spore-forming rods [30] mediterraneus Ovoid [31] oligotrophicus Oval rods [32] Desulfobacter hydrogenophilus Ovoid Gram negative bacteria [33] vibrioformis vibrio-shaped [34] psychrotolerans Rod Gram positive bacteria [35] Desulfococcus biacutus Sphere Gram negative bacteria [36] multivorans [37] oleovorans [38] Desulfomonile limimaris Rod Gram negative bacteria [39] tiedjei Large rod [40]…”